FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_1_AGTTCC_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_1_AGTTCC_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 30072319
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9436750 3.3640876 4.0013566 16
TGTGT 4319730 2.685706 22.09137 4
TGTTT 4504180 2.120504 16.93808 6
GAGGG 1773460 1.9717983 7.393465 11
GTTTG 3138975 1.9515951 21.413141 7
CTTTG 2916105 1.8749189 22.110203 1
TTTGA 3644265 1.759201 16.88462 8
TTTGT 3641200 1.7142252 16.593676 2
TGAGG 2006960 1.6896677 8.751864 10
GTGTT 2656840 1.6518372 21.319187 5
TTGTG 2502720 1.5560162 21.302536 3
TTGAG 2331510 1.4863508 13.379177 9
Produced by FastQC (version 0.10.1)