##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_1_AGTTCC_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	30072319	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9008196208613	34.0	33.0	34.0	31.0	34.0
2	33.11308356365866	34.0	33.0	34.0	31.0	34.0
3	33.166648870677385	34.0	33.0	34.0	31.0	34.0
4	36.49497113940564	37.0	37.0	37.0	35.0	37.0
5	36.44107612718527	37.0	37.0	37.0	35.0	37.0
6	36.41104498791729	37.0	37.0	37.0	35.0	37.0
7	36.40684351612524	37.0	37.0	37.0	35.0	37.0
8	36.40055683766855	37.0	37.0	37.0	35.0	37.0
9	38.21948935830323	39.0	39.0	39.0	37.0	39.0
10	38.206592481278214	39.0	39.0	39.0	37.0	39.0
11	38.19409085145712	39.0	39.0	39.0	37.0	39.0
12	38.15602395013168	39.0	39.0	39.0	37.0	39.0
13	38.15437336242675	39.0	39.0	39.0	37.0	39.0
14	39.71486000131882	41.0	40.0	41.0	38.0	41.0
15	39.67043595806496	41.0	40.0	41.0	37.0	41.0
16	39.65715982861182	41.0	40.0	41.0	37.0	41.0
17	39.64961059371577	41.0	40.0	41.0	37.0	41.0
18	39.625634790585984	41.0	40.0	41.0	37.0	41.0
19	39.61163311017019	41.0	40.0	41.0	37.0	41.0
20	39.5553891936302	41.0	40.0	41.0	37.0	41.0
21	39.54646148173674	41.0	40.0	41.0	37.0	41.0
22	39.51362277714598	41.0	39.0	41.0	37.0	41.0
23	39.46822707620254	41.0	39.0	41.0	37.0	41.0
24	39.46271712534042	41.0	39.0	41.0	37.0	41.0
25	39.41929945608784	41.0	39.0	41.0	37.0	41.0
26	39.3539590012995	41.0	39.0	41.0	36.0	41.0
27	39.278124344185095	41.0	39.0	41.0	36.0	41.0
28	39.231657392301535	40.0	39.0	41.0	36.0	41.0
29	39.17198301201846	40.0	39.0	41.0	36.0	41.0
30	39.12187024884911	40.0	39.0	41.0	36.0	41.0
31	39.06415009098567	40.0	39.0	41.0	36.0	41.0
32	39.01043265070446	40.0	39.0	41.0	36.0	41.0
33	38.93401446692555	40.0	38.0	41.0	35.0	41.0
34	35.72689947855368	40.0	38.0	41.0	24.0	41.0
35	38.79713410196267	40.0	38.0	41.0	35.0	41.0
36	38.74293725069889	40.0	38.0	41.0	35.0	41.0
37	38.89271532401608	40.0	38.0	41.0	35.0	41.0
38	38.92042708778129	40.0	38.0	41.0	35.0	41.0
39	38.90019662268148	40.0	39.0	41.0	35.0	41.0
40	38.86132858593313	40.0	38.0	41.0	35.0	41.0
41	38.821507446765246	40.0	38.0	41.0	35.0	41.0
42	38.767721837481176	40.0	38.0	41.0	35.0	41.0
43	38.72538044039769	40.0	38.0	41.0	35.0	41.0
44	38.67324950895872	40.0	38.0	41.0	35.0	41.0
45	38.61683267592366	40.0	38.0	41.0	35.0	41.0
46	38.54381762178035	40.0	38.0	41.0	35.0	41.0
47	38.488641364837875	40.0	38.0	41.0	35.0	41.0
48	38.428644661557364	40.0	38.0	41.0	34.0	41.0
49	38.38457616121989	40.0	38.0	41.0	34.0	41.0
50	38.501083105695976	40.0	38.0	41.0	35.0	41.0
51	38.21697089605893	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	5.0
10	10.0
11	24.0
12	32.0
13	89.0
14	177.0
15	349.0
16	544.0
17	925.0
18	1515.0
19	2543.0
20	4341.0
21	7430.0
22	12297.0
23	19622.0
24	29267.0
25	41416.0
26	58146.0
27	78450.0
28	104993.0
29	138967.0
30	182520.0
31	239245.0
32	316056.0
33	428015.0
34	615126.0
35	926533.0
36	1445583.0
37	2528243.0
38	6441580.0
39	1.6437583E7
40	10689.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.825817975843382	28.936065623131952	28.075805879962108	23.162310521062558
2	22.38701526715958	28.406226478713535	30.835122252242336	18.371636001884546
3	20.423007979892247	27.246133758700573	32.006747632582304	20.324110628824872
4	21.05926278828388	27.29495248968395	31.393716299355912	20.25206842267626
5	23.726645000887693	27.689340382875155	28.961896474261422	19.62211814197573
6	21.966170949436922	27.396294246546134	30.268081420657982	20.369453383358962
7	23.701544267337677	28.233033840855438	28.049642596568624	20.015779295238257
8	21.14868157182054	27.566153684388556	30.665535738673565	20.61962900511734
9	20.899168928385755	27.456215625710577	31.09546014137016	20.5491553045335
10	21.29679789576587	27.74234005698064	30.557307535877097	20.40355451137639
11	23.335589782750045	27.648333339374325	28.516813086479964	20.49926379139567
12	21.215823096316583	29.82392212585933	28.35143508553497	20.608819692289114
13	22.485505690465708	27.919340041584423	28.849916762322188	20.745237505627685
14	22.084587224252626	28.027424962077337	29.00829310507198	20.879694708598056
15	22.13042831848119	27.994412402980963	28.84120443122461	21.03395484731324
16	22.079334819506272	27.93071262645225	28.87832162195406	21.111630932087415
17	22.100390728097825	28.022910371494795	28.8132584653681	21.06344043503928
18	21.967012254691763	27.950255515711973	28.887459593654885	21.19527263594138
19	21.787866590652065	28.162208922918698	28.988358911998024	21.061565574431214
20	21.69739886039384	28.155703589071397	29.007207591805606	21.139689958729154
21	21.768312265900633	28.106582013414	28.9158419782599	21.20926374242547
22	21.753483660505196	28.153322661947023	28.921544095086247	21.171649582461534
23	21.79784671744138	28.210012669791112	28.799388567273443	21.192752045494064
24	21.712884862653926	28.201702702076286	28.790819224816016	21.294593210453776
25	21.771979890198565	28.280916312401438	28.716183524517174	21.23092027288282
26	21.783770649679525	28.291173687004317	28.694943678936102	21.230111984380056
27	21.807586571557717	28.216094674973352	28.600448139699502	21.375870613769425
28	21.794301843421486	28.205177743880938	28.668946927781047	21.33157348491653
29	21.787282184656263	28.304554763468694	28.62453341227193	21.28362963960312
30	21.794185543190068	28.238653626945098	28.560238403962128	21.406922425902707
31	21.893163610029543	28.20968346338704	28.515705755848092	21.38144717073532
32	21.8753258855841	28.29308380340666	28.44563816112209	21.38595214988715
33	21.875459518876795	28.23050208408333	28.41121707899525	21.482821318044625
34	21.901882440606784	28.280269830937588	28.37549855083808	21.44234917761755
35	21.85382642415376	28.285789793615017	28.407782512960228	21.452601269270993
36	21.86720129813099	28.276886014041292	28.35534546718492	21.500567220642793
37	21.91292458904157	28.266039557506144	28.358633421775924	21.46240243167636
38	21.856046953151896	28.309225569018093	28.384817866358524	21.449909611471487
39	21.842435272208863	28.184777982440195	28.414784603606115	21.558002141744826
40	21.898815478359545	28.228313471811184	28.373276855521002	21.499594194308266
41	21.87909403108495	28.222048291397893	28.358803498844985	21.540054178672165
42	21.857033582221696	28.17693294725426	28.318392261357488	21.64764120916655
43	21.890654917837104	28.276123033893207	28.269611868358336	21.56361017991135
44	21.911484357167673	28.261145860545987	28.23649485205271	21.59087493023362
45	21.9245790657303	28.196437500024942	28.28364178724096	21.595341647003803
46	21.872279474583866	28.27533137121863	28.268132058734686	21.584257095462817
47	21.904741540791758	28.290315048131827	28.235439690878355	21.569503720198064
48	21.892940813776285	28.238427505374624	28.2381348774599	21.63049680338919
49	21.890425813685873	28.327978676477798	28.164061831031606	21.61753367880472
50	21.86662482898415	28.266546562899812	28.22361593965158	21.643212668464454
51	21.92385961322105	28.22229639157526	28.202690321288493	21.651153673915204
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.98812849690594
2	40.75865126904413
3	40.74711860871712
4	41.31133121096014
5	43.34876314286342
6	42.33562433279589
7	43.71732356257594
8	41.768310576937886
9	41.44832423291926
10	41.70035240714226
11	43.83485357414571
12	41.82464278860569
13	43.23074319609339
14	42.96428193285068
15	43.164383165794426
16	43.19096575159369
17	43.163831163137104
18	43.162284890633146
19	42.84943216508328
20	42.83708881912299
21	42.97757600832611
22	42.92513324296673
23	42.99059876293544
24	43.0074780731077
25	43.00290016308138
26	43.01388263405958
27	43.18345718532714
28	43.12587532833801
29	43.070911824259376
30	43.201107969092774
31	43.27461078076486
32	43.26127803547125
33	43.35828083692142
34	43.344231618224335
35	43.30642769342475
36	43.36776851877379
37	43.37532702071793
38	43.30595656462338
39	43.40043741395369
40	43.39840967266781
41	43.41914820975711
42	43.50467479138825
43	43.454265097748454
44	43.5023592874013
45	43.5199207127341
46	43.45653657004668
47	43.47424526098982
48	43.52343761716547
49	43.50795949249059
50	43.50983749744861
51	43.57501328713626
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21177.0
1	13083.0
2	4989.0
3	7447.0
4	9905.0
5	12394.0
6	14883.0
7	22239.0
8	29595.0
9	24730.5
10	19866.0
11	22350.5
12	24835.0
13	32935.0
14	41035.0
15	61968.0
16	82901.0
17	138915.5
18	194930.0
19	239830.0
20	284730.0
21	325579.0
22	366428.0
23	427212.5
24	487997.0
25	585848.0
26	809718.0
27	935737.0
28	1002781.5
29	1069826.0
30	1118078.5
31	1166331.0
32	1247288.0
33	1328245.0
34	1416624.5
35	1505004.0
36	1669969.0
37	1834934.0
38	1894694.0
39	1954454.0
40	2015626.0
41	2076798.0
42	2069665.0
43	2062532.0
44	2058612.0
45	2054692.0
46	1995818.0
47	1936944.0
48	1881444.5
49	1825945.0
50	1744012.5
51	1662080.0
52	1556807.0
53	1451534.0
54	1340302.5
55	1229071.0
56	1114402.0
57	999733.0
58	891754.0
59	783775.0
60	683794.0
61	583813.0
62	497465.5
63	411118.0
64	346568.0
65	282018.0
66	234821.0
67	187624.0
68	160234.0
69	132844.0
70	112917.0
71	92990.0
72	77964.0
73	62938.0
74	53291.5
75	36362.5
76	29080.0
77	26917.0
78	24754.0
79	23535.5
80	22317.0
81	19116.5
82	15916.0
83	14409.5
84	12903.0
85	11546.5
86	10190.0
87	11050.5
88	11911.0
89	7427.0
90	2943.0
91	1737.5
92	532.0
93	340.0
94	148.0
95	84.0
96	20.0
97	13.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.2752431563392234
2	0.008253437322209838
3	0.002444108151419915
4	2.660253770252969E-5
5	3.3253172128162115E-5
6	0.0
7	0.0
8	8.64582475332215E-4
9	2.028443499817889E-4
10	0.0
11	0.0
12	0.0
13	0.0
14	0.001965262472774381
15	0.0
16	0.0
17	0.0
18	0.0
19	0.003002761443173039
20	0.0
21	5.087735335608804E-4
22	0.0
23	0.0
24	0.0
25	7.215938351811178E-4
26	0.0
27	0.0
28	1.0641015081011877E-4
29	0.0
30	0.0
31	0.0
32	0.0013800066433187277
33	3.4915830734570224E-4
34	8.49056569265576
35	0.005559930379828706
36	0.006683887597760585
37	0.004030284461933248
38	0.0015695497244492516
39	0.0019153827145821378
40	0.0042464300807663015
41	0.0011239572179318794
42	2.261215704715024E-4
43	0.0029861348571089577
44	0.0017524421711541433
45	0.0013401028367649333
46	9.975951638448634E-6
47	0.0017723940744310406
48	0.0
49	0.001210415465465101
50	0.0018122978809848353
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.0072319E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.701528247415396
#Duplication Level	Relative count
1	100.0
2	4.355982587203153
3	1.3671940235362228
4	0.7776751021498792
5	0.55166501519723
6	0.43698994644785627
7	0.3429118560660885
8	0.2738841447800577
9	0.23770026386399315
10++	2.9910152640309957
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	9436750	3.3640876	4.0013566	16
TGTGT	4319730	2.685706	22.09137	4
TGTTT	4504180	2.120504	16.93808	6
GAGGG	1773460	1.9717983	7.393465	11
GTTTG	3138975	1.9515951	21.413141	7
CTTTG	2916105	1.8749189	22.110203	1
TTTGA	3644265	1.759201	16.88462	8
TTTGT	3641200	1.7142252	16.593676	2
TGAGG	2006960	1.6896677	8.751864	10
GTGTT	2656840	1.6518372	21.319187	5
TTGTG	2502720	1.5560162	21.302536	3
TTGAG	2331510	1.4863508	13.379177	9
>>END_MODULE