FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_15_TGACCA_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_15_TGACCA_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 28082252
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 118065 0.42042568380911904 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6169850 3.805641 34.282314 4
TTTTT 9397180 3.318474 4.1365075 17
GTTTG 4597140 2.8355737 33.77171 7
TGTTT 5827800 2.719892 26.143421 6
GTGTT 4179170 2.5777645 33.651077 5
CTTTG 3696285 2.4499464 36.277718 1
GGGGG 1714445 2.4411542 5.4707627 13
TTTGA 4981405 2.440078 27.02467 8
TTGTG 3827690 2.3609674 33.629784 3
GAGGG 2028545 2.2937968 11.041254 11
TTTGT 4434715 2.0697255 25.761124 2
TTGAG 3192635 2.0668423 21.909908 9
TGAGG 2302285 1.969805 13.296688 10
AGGGG 1500845 1.6970949 6.014607 12
AACTT 2838680 1.5682344 11.498097 2
TGAGC 1629540 1.4981908 5.8539505 10
ACTTT 2747660 1.4462814 10.49847 3
TGAGT 1936870 1.253887 6.6325235 10
GAGTG 1437935 1.2302784 5.427689 11
TAACT 1980405 1.0940787 11.189317 1
TTGAT 2214415 1.0847031 5.959948 9
TTGAC 1425540 0.9916887 5.4171352 9
Produced by FastQC (version 0.10.1)