##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_15_TGACCA_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28082252	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29709835949054	34.0	31.0	34.0	31.0	34.0
2	32.47720286108108	34.0	31.0	34.0	31.0	34.0
3	32.55156249577135	34.0	31.0	34.0	31.0	34.0
4	35.916336303797856	37.0	37.0	37.0	35.0	37.0
5	35.930288176318626	37.0	37.0	37.0	35.0	37.0
6	35.91644206454667	37.0	37.0	37.0	35.0	37.0
7	35.942592816274136	37.0	37.0	37.0	35.0	37.0
8	35.94853984644821	37.0	37.0	37.0	35.0	37.0
9	37.72217979526713	39.0	39.0	39.0	35.0	39.0
10	37.70793503313053	39.0	39.0	39.0	35.0	39.0
11	37.70931010091356	39.0	39.0	39.0	35.0	39.0
12	37.66979428857771	39.0	38.0	39.0	35.0	39.0
13	37.66782019476216	39.0	38.0	39.0	35.0	39.0
14	39.175518473375995	41.0	40.0	41.0	36.0	41.0
15	39.14009360787732	41.0	40.0	41.0	36.0	41.0
16	39.11834834328814	41.0	40.0	41.0	36.0	41.0
17	39.08594720252493	41.0	40.0	41.0	36.0	41.0
18	39.06085523340507	41.0	39.0	41.0	36.0	41.0
19	39.072313502492605	41.0	40.0	41.0	36.0	41.0
20	39.04205228982348	41.0	40.0	41.0	36.0	41.0
21	39.011272137291556	41.0	39.0	41.0	36.0	41.0
22	38.9765055167228	41.0	39.0	41.0	36.0	41.0
23	38.94618074077535	41.0	39.0	41.0	36.0	41.0
24	38.919690913677435	41.0	39.0	41.0	36.0	41.0
25	38.8711517153254	41.0	39.0	41.0	36.0	41.0
26	38.846873142510084	41.0	39.0	41.0	36.0	41.0
27	38.80063464995614	41.0	39.0	41.0	36.0	41.0
28	38.76599024180824	41.0	39.0	41.0	35.0	41.0
29	38.71718521719697	41.0	39.0	41.0	35.0	41.0
30	38.67888056128832	41.0	39.0	41.0	35.0	41.0
31	38.63285394632881	41.0	39.0	41.0	35.0	41.0
32	38.586445702431554	41.0	39.0	41.0	35.0	41.0
33	38.54013567003102	40.0	39.0	41.0	35.0	41.0
34	38.49767422498737	40.0	39.0	41.0	35.0	41.0
35	38.45045472136636	40.0	38.0	41.0	35.0	41.0
36	38.41072820655551	40.0	38.0	41.0	34.0	41.0
37	38.36348844102674	40.0	38.0	41.0	34.0	41.0
38	38.32553521704741	40.0	38.0	41.0	34.0	41.0
39	38.282967192232306	40.0	38.0	41.0	34.0	41.0
40	38.22178773269323	40.0	38.0	41.0	34.0	41.0
41	38.15405484574385	40.0	38.0	41.0	34.0	41.0
42	38.078633152355444	40.0	38.0	41.0	33.0	41.0
43	38.044555935186395	40.0	38.0	41.0	33.0	41.0
44	37.987020734661876	40.0	38.0	41.0	33.0	41.0
45	37.933909716357505	40.0	38.0	41.0	33.0	41.0
46	37.87945147703966	40.0	38.0	41.0	33.0	41.0
47	37.82215493258874	40.0	38.0	41.0	33.0	41.0
48	38.02758051597856	40.0	38.0	41.0	33.0	41.0
49	38.074994590889645	40.0	38.0	41.0	33.0	41.0
50	38.0550189137253	40.0	38.0	41.0	33.0	41.0
51	36.548941445294346	39.0	36.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	126299.0
3	5507.0
4	2758.0
5	1377.0
6	2315.0
7	3982.0
8	8352.0
9	12160.0
10	13604.0
11	15786.0
12	20718.0
13	26289.0
14	31259.0
15	34597.0
16	36112.0
17	37026.0
18	38174.0
19	39745.0
20	41770.0
21	44764.0
22	48838.0
23	54434.0
24	62107.0
25	71776.0
26	82839.0
27	97354.0
28	116457.0
29	140280.0
30	170408.0
31	215096.0
32	277743.0
33	370161.0
34	528546.0
35	787044.0
36	1194563.0
37	2001477.0
38	4676286.0
39	1.6632461E7
40	11788.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.932684320036255	27.606057340869484	30.54340324329638	22.917855095797883
2	21.281228690543664	29.13508344500288	31.86814248986382	17.715545374589635
3	21.873140718412625	28.050574821589503	31.2118913270024	18.86439313299547
4	21.1081540153587	26.15979112077158	32.36130124875693	20.37075361511279
5	25.802813826474736	26.2578684781286	29.086798422367448	18.852519273029213
6	20.915364181409377	25.92738292395526	33.764952298127085	19.392300596508274
7	24.56693693893574	26.32345142148804	30.17420859656192	18.9354030430143
8	21.831328791891934	26.455676235408575	32.471983217293484	19.241011755406
9	21.847931735520575	26.61864666592496	32.28547061687516	19.247950981679303
10	21.77517966488694	27.85056529275492	31.23680891749732	19.13744612486082
11	25.218471641695345	26.42346844430501	28.974399838098126	19.383660075901524
12	20.974776990286202	30.321102932800276	29.1132612569602	19.590858819953322
13	23.445224348257355	26.77823011984288	29.7365849065628	20.03996062533696
14	23.677755225380576	27.13043964106682	28.86569565767311	20.32610947587949
15	22.988505171257593	28.25884383657002	28.307505823388336	20.445145168784055
16	23.172166149346086	27.454700462082478	28.678689540393222	20.694443848178214
17	23.050164048244106	27.51876686362523	28.792212225291607	20.63885686283905
18	22.771284358557942	27.46148742324504	28.932087026291548	20.83514119190547
19	22.41821835758475	27.799688260000465	29.196963487765725	20.58512989464906
20	22.16720127159601	27.81318779668784	29.238621148875538	20.780989782840614
21	22.221681724538396	27.924651759311015	29.138992558850262	20.714673957300324
22	21.983481709775273	28.047134571383342	29.318355650222962	20.651028068618423
23	21.99401302398345	28.15225004507575	29.169918557953988	20.68381837298681
24	21.887811383564888	28.202834478361023	29.131012442649705	20.77834169542439
25	21.923927844659026	28.33302739374811	29.009538763046695	20.73350599854617
26	21.877932541215326	28.324531160336775	29.046573059229143	20.750963239218756
27	21.905576616364158	28.282098385876846	28.90999074673637	20.902334251022626
28	21.864966225072138	28.29452534634662	28.957405757933707	20.88310267064753
29	21.90927259930647	28.308040576774097	28.917392493939815	20.865294329979623
30	21.911444049511662	28.310872997945545	28.777050399811273	21.00063255273152
31	22.000749365786994	28.241469788081293	28.76074841341576	20.997032432715955
32	21.976213436746697	28.315696679346953	28.666187932930292	21.04190195097606
33	21.982976740056817	28.27830742531158	28.628606264891864	21.110109569739734
34	22.017963639232992	28.284794526501837	28.59313061717269	21.10411121709248
35	21.97053746751135	28.286759990332122	28.60628249379495	21.136420048361575
36	21.949218955181294	28.35094245164142	28.586380363472024	21.113458229705266
37	22.056881624361903	28.22987017813477	28.59595123969824	21.117296957805085
38	21.936971941258108	28.314945070826305	28.607815203136	21.14026778477959
39	21.93504189249086	28.202474423755174	28.63092031235115	21.231563371402814
40	21.949791040060745	28.255330764504155	28.581214178155474	21.213664017279626
41	21.991097274124353	28.22971618241689	28.507086173738337	21.272100369720416
42	21.91733754494982	28.175651949247086	28.558665347658618	21.348345158144475
43	21.96100245207282	28.276409988408506	28.501184225273825	21.26140333424485
44	21.976690383041724	28.275935690154895	28.419148537455985	21.328225389347395
45	21.984394843395133	28.23974028316246	28.485441602353074	21.290423271089335
46	21.88769662947096	28.356314256546106	28.402399512586925	21.353589601396013
47	21.9953932420469	28.313793265417942	28.32614468676939	21.36466880576577
48	21.927762564287363	28.322480406999507	28.37160992622516	21.378147102487965
49	21.91557977077506	28.39082494342679	28.30310169039776	21.39049359540039
50	21.920469637248043	28.33918309340277	28.36363232776653	21.376714941582655
51	21.96849718715298	28.288935359549587	28.36287560925631	21.379691844041123
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.850539415834135
2	38.9967740651333
3	40.737533851408095
4	41.47890763047149
5	44.65533309950395
6	40.30766477791765
7	43.50233998195004
8	41.072340547297934
9	41.09588271719988
10	40.91262578974776
11	44.60213171759687
12	40.56563581023953
13	43.485184973594315
14	44.00386470126006
15	43.433650340041645
16	43.8666099975243
17	43.68902091108316
18	43.60642555046341
19	43.003348252233806
20	42.94819105443663
21	42.93635568183872
22	42.6345097783937
23	42.677831396970255
24	42.666153078989275
25	42.6574338432052
26	42.628895780434085
27	42.80791086738678
28	42.74806889571967
29	42.77456692928609
30	42.91207660224318
31	42.99778179850295
32	43.01811538772276
33	43.093086309796554
34	43.12207485632547
35	43.10695751587293
36	43.06267718488656
37	43.17417858216699
38	43.077239726037696
39	43.16660526389368
40	43.16345505734037
41	43.26319764384477
42	43.26568270309429
43	43.22240578631767
44	43.304915772389116
45	43.27481811448447
46	43.241286230866976
47	43.360062047812676
48	43.305909666775335
49	43.306073366175454
50	43.2971845788307
51	43.3481890311941
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132981.0
1	71057.0
2	9133.0
3	9541.5
4	9950.0
5	17925.5
6	25901.0
7	30942.0
8	35983.0
9	29020.0
10	22057.0
11	23643.5
12	25230.0
13	31985.0
14	38740.0
15	55995.0
16	73250.0
17	117835.5
18	162421.0
19	201171.0
20	239921.0
21	280191.5
22	320462.0
23	381478.5
24	442495.0
25	525323.0
26	723283.5
27	838416.0
28	910997.0
29	983578.0
30	1041611.5
31	1099645.0
32	1186834.5
33	1274024.0
34	1352681.0
35	1431338.0
36	1589588.5
37	1747839.0
38	1812825.0
39	1877811.0
40	1947620.5
41	2017430.0
42	2007611.5
43	1997793.0
44	1985869.5
45	1973946.0
46	1909007.0
47	1844068.0
48	1774962.5
49	1705857.0
50	1618266.5
51	1530676.0
52	1419596.5
53	1308517.0
54	1203437.5
55	1098358.0
56	988864.0
57	879370.0
58	778897.0
59	678424.0
60	589958.5
61	501493.0
62	425922.5
63	350352.0
64	294337.5
65	238323.0
66	199004.0
67	159685.0
68	135868.0
69	112051.0
70	96588.5
71	81126.0
72	68065.5
73	55005.0
74	46426.0
75	31769.0
76	25691.0
77	23469.5
78	21248.0
79	19949.0
80	18650.0
81	16306.5
82	13963.0
83	12409.5
84	10856.0
85	9323.5
86	7791.0
87	8164.5
88	8538.0
89	5859.0
90	3180.0
91	2231.0
92	1282.0
93	1020.5
94	759.0
95	569.0
96	379.0
97	294.0
98	209.0
99	134.0
100	59.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8889707278461856
2	0.5293165234753965
3	0.5153219193389476
4	0.5107425145248322
5	0.5044182354036278
6	0.5002340980345878
7	0.4899607054306043
8	0.4447720218449717
9	0.4580188227069538
10	0.46170086359170914
11	0.4788077537371291
12	0.4696916757245822
13	0.4566407281011509
14	0.4473572845938424
15	0.49308367434349637
16	0.46482027153662747
17	0.48827636757906734
18	0.4965128865021224
19	0.5067328645865011
20	0.5300572048139159
21	0.5164721119944369
22	0.5494609193023409
23	0.5197054709145121
24	0.5456578055064815
25	0.5409145961655781
26	0.5265318465200013
27	0.5281770137238282
28	0.5209446877693427
29	0.5398213789976672
30	0.5263894078010553
31	0.5411496200518392
32	0.5647267890053832
33	0.5253567270886964
34	0.5367019710527489
35	0.5361856306965695
36	0.5464127307168956
37	0.5406475235675543
38	0.5526551075747059
39	0.5313071045726675
40	0.5413312294184953
41	0.5594173857566694
42	0.5994747144922707
43	0.5238575595717893
44	0.5604358225971335
45	0.565901908436688
46	0.5468934613933384
47	0.5350140722332383
48	0.5412564490910486
49	0.5588262650730433
50	0.537374993999769
51	0.5109170019555411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8082252E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.200351659392755
#Duplication Level	Relative count
1	100.0
2	12.344181922495176
3	3.7018202680853283
4	1.8111406665623533
5	1.0933343764669066
6	0.7790903875241225
7	0.5919783028216763
8	0.48114536066343294
9	0.387263339070568
10++	9.201742033067335
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	118065	0.42042568380911904	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6169850	3.805641	34.282314	4
TTTTT	9397180	3.318474	4.1365075	17
GTTTG	4597140	2.8355737	33.77171	7
TGTTT	5827800	2.719892	26.143421	6
GTGTT	4179170	2.5777645	33.651077	5
CTTTG	3696285	2.4499464	36.277718	1
GGGGG	1714445	2.4411542	5.4707627	13
TTTGA	4981405	2.440078	27.02467	8
TTGTG	3827690	2.3609674	33.629784	3
GAGGG	2028545	2.2937968	11.041254	11
TTTGT	4434715	2.0697255	25.761124	2
TTGAG	3192635	2.0668423	21.909908	9
TGAGG	2302285	1.969805	13.296688	10
AGGGG	1500845	1.6970949	6.014607	12
AACTT	2838680	1.5682344	11.498097	2
TGAGC	1629540	1.4981908	5.8539505	10
ACTTT	2747660	1.4462814	10.49847	3
TGAGT	1936870	1.253887	6.6325235	10
GAGTG	1437935	1.2302784	5.427689	11
TAACT	1980405	1.0940787	11.189317	1
TTGAT	2214415	1.0847031	5.959948	9
TTGAC	1425540	0.9916887	5.4171352	9
>>END_MODULE