##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_15_TGACCA_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	28082252	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94653719366951	34.0	33.0	34.0	31.0	34.0
2	33.16238377178583	34.0	34.0	34.0	31.0	34.0
3	33.20242176446533	34.0	34.0	34.0	31.0	34.0
4	36.52651970361921	37.0	37.0	37.0	35.0	37.0
5	36.45759706166015	37.0	37.0	37.0	35.0	37.0
6	36.41281507622679	37.0	37.0	37.0	35.0	37.0
7	36.43074362412245	37.0	37.0	37.0	35.0	37.0
8	36.41201414331016	37.0	37.0	37.0	35.0	37.0
9	38.23922796505067	39.0	39.0	39.0	37.0	39.0
10	38.217449547849654	39.0	39.0	39.0	37.0	39.0
11	38.190051816357176	39.0	39.0	39.0	37.0	39.0
12	38.15382608916123	39.0	39.0	39.0	37.0	39.0
13	38.15334396970727	39.0	39.0	39.0	37.0	39.0
14	39.71080827135943	41.0	40.0	41.0	37.0	41.0
15	39.69085684438698	41.0	40.0	41.0	37.0	41.0
16	39.67262191080687	41.0	40.0	41.0	37.0	41.0
17	39.65738538347993	41.0	40.0	41.0	37.0	41.0
18	39.62217072192073	41.0	40.0	41.0	37.0	41.0
19	39.6269999286382	41.0	40.0	41.0	37.0	41.0
20	39.60619397618111	41.0	40.0	41.0	37.0	41.0
21	39.54762691396687	41.0	40.0	41.0	37.0	41.0
22	39.515079168152184	41.0	39.0	41.0	37.0	41.0
23	39.47959917886927	41.0	39.0	41.0	37.0	41.0
24	39.470509914945566	41.0	39.0	41.0	37.0	41.0
25	39.40912762267072	41.0	39.0	41.0	37.0	41.0
26	39.33424929738541	41.0	39.0	41.0	36.0	41.0
27	39.28616565366624	41.0	39.0	41.0	36.0	41.0
28	39.23610560150233	41.0	39.0	41.0	36.0	41.0
29	39.18513807226002	40.0	39.0	41.0	36.0	41.0
30	39.126703513664076	40.0	39.0	41.0	36.0	41.0
31	39.057032569895036	40.0	39.0	41.0	36.0	41.0
32	38.99567420732497	40.0	39.0	41.0	36.0	41.0
33	38.931477539621824	40.0	39.0	41.0	35.0	41.0
34	38.87135757488395	40.0	38.0	41.0	35.0	41.0
35	38.80919087258386	40.0	38.0	41.0	35.0	41.0
36	38.739017654282144	40.0	38.0	41.0	35.0	41.0
37	38.92461929335297	40.0	39.0	41.0	35.0	41.0
38	38.95656106924758	40.0	39.0	41.0	35.0	41.0
39	38.94265588101695	40.0	39.0	41.0	35.0	41.0
40	38.91436231681134	40.0	39.0	41.0	35.0	41.0
41	38.86725039003282	40.0	39.0	41.0	35.0	41.0
42	38.81190920870591	40.0	38.0	41.0	35.0	41.0
43	38.77872629303376	40.0	38.0	41.0	35.0	41.0
44	38.72926530251206	40.0	38.0	41.0	35.0	41.0
45	38.669696646835874	40.0	38.0	41.0	35.0	41.0
46	38.620673477326534	40.0	38.0	41.0	35.0	41.0
47	38.54975808207974	40.0	38.0	41.0	35.0	41.0
48	38.500803496813575	40.0	38.0	41.0	35.0	41.0
49	38.45227604965584	40.0	38.0	41.0	34.0	41.0
50	38.54458616780449	40.0	38.0	41.0	35.0	41.0
51	38.29296578493776	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	10.0
11	41.0
12	76.0
13	155.0
14	276.0
15	472.0
16	736.0
17	1097.0
18	1736.0
19	2877.0
20	4981.0
21	8140.0
22	13337.0
23	20611.0
24	30234.0
25	42056.0
26	57458.0
27	76432.0
28	101239.0
29	132529.0
30	171054.0
31	222407.0
32	288744.0
33	382928.0
34	540806.0
35	802163.0
36	1218846.0
37	2075503.0
38	4959476.0
39	1.6909596E7
40	16230.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.477288756383306	28.490394011768394	28.83651081073592	23.195806421112376
2	21.40711985639929	28.02444998450294	32.01077069424994	18.557659464847823
3	20.51431949873787	27.128220181066087	32.874540283174774	19.482920037021273
4	21.114528136846005	26.936635993438134	32.43546849447829	19.51336737523757
5	24.872848480342242	27.38155825439021	28.618065564845885	19.127527700421663
6	21.486083096184736	27.012100026735748	31.58383451583584	19.917982361243677
7	24.775717671934352	27.56108932943976	28.09661349860775	19.566579500018143
8	20.987398019218688	27.43902447709678	31.57058059303791	20.002996910646626
9	20.535069623333627	27.419275348714912	32.068457330273944	19.977197697677525
10	20.93587437360793	27.469648801670175	31.765721637993988	19.828755186727903
11	24.48979875260716	27.337344597577147	28.26617323995241	19.906683409863284
12	20.8729495412862	31.06440967305159	28.018120705918225	20.044520079743982
13	23.02384082302231	27.709608189542635	28.843577074943987	20.422973912491063
14	22.29769675239531	27.85316407483267	29.169878371952258	20.679260800819765
15	22.49412547113387	27.848539355034628	28.873264864940317	20.78407030889118
16	22.33305576774968	27.83988620285866	28.87716056390349	20.949897465488164
17	22.427827369400433	27.97831527186637	28.720762850500737	20.87309450823246
18	22.131586882704422	27.91864057056393	28.944829638306786	21.004942908424866
19	21.80205736713247	28.252697350961398	29.177216966006704	20.768028315899432
20	21.61881461643461	28.209653556274617	29.23554350270769	20.935988324583086
21	21.784000798796335	28.241022123154508	29.047513710794988	20.927463367254166
22	21.708141498053646	28.2662017276962	29.125947591382626	20.89970918286753
23	21.758390317129837	28.324790333766682	28.989840273493733	20.926979075609747
24	21.621838167009926	28.35531999985614	28.9806154044582	21.04222642867574
25	21.697512008652296	28.469728852230226	28.887451761347343	20.94530737777013
26	21.698759421235366	28.423542810054325	28.85783173547662	21.019866033233686
27	21.719636302672594	28.389592828951184	28.74949630108013	21.141274567296097
28	21.695998597263497	28.387741125604883	28.847932138775768	21.068328138355856
29	21.722913434943962	28.43860311164842	28.786990846653808	21.051492606753815
30	21.69458320196287	28.394790258670255	28.74489150799714	21.165735031369735
31	21.807407041287146	28.367308291372073	28.663256778694247	21.162027888646538
32	21.804123828815438	28.45012572353528	28.585645481708514	21.160104965940764
33	21.810189768781516	28.377624751769154	28.548006538085456	21.264178941363873
34	21.828406069427768	28.382574161075112	28.54342664541291	21.24559312408421
35	21.77720851309855	28.41775284876761	28.51607480901357	21.288963829120274
36	21.782909737287444	28.471467959563224	28.485872172986166	21.259750130163166
37	21.870524855377212	28.382975645812277	28.48578812915359	21.26071136965692
38	21.78632254991516	28.392391749778472	28.5301299909993	21.291155709307073
39	21.762248571164434	28.369524233333195	28.53742834811467	21.3307988473877
40	21.77921945975813	28.41592432544194	28.51278310467233	21.292073110127603
41	21.812472291215983	28.34793152258099	28.478309252285694	21.36128693391733
42	21.77302732996375	28.29814226212474	28.465988690779337	21.46284171713217
43	21.789920582483905	28.40560528374219	28.42853794525774	21.375936188516164
44	21.796282908921093	28.376380995194328	28.393349151999892	21.433986943884687
45	21.833329938539027	28.320482731203654	28.458972533930023	21.387214796327296
46	21.73744423465145	28.417182299137117	28.41861025100652	21.426763215204918
47	21.81640692400651	28.45738868142454	28.321602899023606	21.404601495545347
48	21.758446533626426	28.40055437192844	28.374648309624984	21.46635078482015
49	21.81296793884746	28.459203704085535	28.274847687900497	21.452980669166507
50	21.764100507838	28.398029470343594	28.341138510045123	21.496731511773287
51	21.82996221243225	28.38294450174438	28.30965265891069	21.477440626912685
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.67309517749568
2	39.964779321247114
3	39.997239535759135
4	40.627895512083576
5	44.0003761807639
6	41.404065457428416
7	44.3422971719525
8	40.99039492986531
9	40.512267321011144
10	40.76462956033583
11	44.39648216247045
12	40.917469621030186
13	43.44681473551338
14	42.97695755321507
15	43.278195780025044
16	43.28295323323785
17	43.300921877632895
18	43.136529791129284
19	42.5700856830319
20	42.55480294101769
21	42.7114641660505
22	42.607850680921175
23	42.68536939273959
24	42.664064595685666
25	42.642819386422424
26	42.71862545446905
27	42.86091086996869
28	42.76432673561935
29	42.77440604169777
30	42.860318233332606
31	42.96943492993368
32	42.9642287947562
33	43.074368710145386
34	43.07399919351197
35	43.06617234221882
36	43.04265986745061
37	43.13123622503414
38	43.07747825922223
39	43.09304741855213
40	43.071292569885735
41	43.17375922513331
42	43.235869047095925
43	43.165856771000065
44	43.23026985280578
45	43.22054473486633
46	43.16420744985637
47	43.22100841955185
48	43.224797318446576
49	43.26594860801397
50	43.260832019611286
51	43.30740283934494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17328.0
1	10540.0
2	3752.0
3	5079.5
4	6407.0
5	9292.0
6	12177.0
7	19698.5
8	27220.0
9	22192.0
10	17164.0
11	19561.0
12	21958.0
13	29414.5
14	36871.0
15	56253.5
16	75636.0
17	124213.0
18	172790.0
19	214505.5
20	256221.0
21	296350.5
22	336480.0
23	399485.5
24	462491.0
25	553166.0
26	765204.0
27	886567.0
28	951563.0
29	1016559.0
30	1068443.0
31	1120327.0
32	1202328.5
33	1284330.0
34	1360055.0
35	1435780.0
36	1590194.5
37	1744609.0
38	1801300.5
39	1857992.0
40	1920291.0
41	1982590.0
42	1973692.0
43	1964794.0
44	1956509.5
45	1948225.0
46	1884095.5
47	1819966.0
48	1760366.5
49	1700767.0
50	1614593.5
51	1528420.0
52	1425602.5
53	1322785.0
54	1217621.5
55	1112458.0
56	1003608.0
57	894758.0
58	794344.0
59	693930.0
60	604189.5
61	514449.0
62	435935.5
63	357422.0
64	300006.0
65	242590.0
66	202206.5
67	161823.0
68	138073.5
69	114324.0
70	98292.0
71	82260.0
72	69006.5
73	55753.0
74	46861.0
75	31601.5
76	25234.0
77	23221.5
78	21209.0
79	19996.5
80	18784.0
81	16473.5
82	14163.0
83	12504.5
84	10846.0
85	9320.0
86	7794.0
87	8213.0
88	8632.0
89	5649.5
90	2667.0
91	1700.0
92	733.0
93	519.5
94	306.0
95	186.5
96	67.0
97	47.0
98	27.0
99	17.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27201522157126146
2	0.009703637728199292
3	6.76583914993712E-5
4	0.0
5	2.8487743789208928E-5
6	0.0
7	0.002823847603105335
8	0.0
9	0.0
10	0.0
11	0.0
12	0.006726668502226959
13	0.0
14	2.777555019447871E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.008831200574654767
20	0.0
21	0.0
22	0.0
23	0.0
24	1.780483986825558E-5
25	0.0
26	0.00501028193892712
27	0.0
28	0.0
29	0.0021686294959535296
30	3.2048711762860043E-4
31	0.0
32	0.0
33	8.90241993412779E-5
34	0.0
35	7.478032744667344E-5
36	6.801448829673631E-4
37	2.1009711044541585E-4
38	0.0
39	0.0047859409565871
40	0.004141405753356248
41	7.121935947302232E-6
42	1.2107291110413794E-4
43	1.2107291110413794E-4
44	8.510713457026168E-4
45	1.4243871894604465E-4
46	2.5995066207653146E-4
47	0.009717881600093896
48	7.834129542032456E-5
49	0.004483258678826756
50	0.0016451672038268156
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8082252E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.22919727527716
#Duplication Level	Relative count
1	100.0
2	12.823544869268165
3	3.9147286821705425
4	1.8847720404677442
5	1.110892129812114
6	0.7896465641834187
7	0.5853370122191565
8	0.4946787544343713
9	0.39350939429772697
10++	9.391669951386152
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	5001785	3.2182586	35.068977	4
TTTTT	8774290	3.1822069	3.8452485	17
TGTTT	4828280	2.3323765	26.422468	6
GTTTG	3580280	2.303631	34.324505	7
CTTTG	3243405	2.186028	35.829945	1
GAGGG	1805445	2.1288755	11.154939	11
GTGTT	3154995	2.0299933	34.25511	5
TTTGA	4012750	2.0023448	26.83942	8
TTTGT	3961235	1.9135367	26.066515	2
TTGTG	2907670	1.8708588	34.20751	3
TGAGG	2039580	1.8055811	13.691679	10
TTGAG	2573955	1.7107534	21.906559	9
AGGGG	1334860	1.5739892	6.1175103	12
TGAGC	1531315	1.4200362	5.6313176	10
TGAGT	1795540	1.1933879	6.6652927	10
GAGTG	1334025	1.1809736	5.417924	11
TTGAT	2038350	1.0171278	6.018059	9
TTGAC	1334960	0.92942286	5.2695074	9
>>END_MODULE