FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_14_CAGATC_L005_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_14_CAGATC_L005_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29304156
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 132767 0.4530654286716191 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 5295890 3.2946596 24.553665 4
TTTTT 8638840 3.0448406 3.6739838 17
TGTTT 5134380 2.4042432 18.670685 6
GTTTG 3780510 2.351917 23.887064 7
CTTTG 3274815 2.1435437 25.183403 1
GTGTT 3370600 2.0969052 23.770409 5
GAGGG 1856320 2.094354 7.9364734 11
TTTGA 4225540 2.0329928 18.68961 8
TTGTG 3132705 1.9489069 23.772936 3
TTTGT 4031680 1.8878889 18.291512 2
TGAGG 2127795 1.8069487 9.390667 10
TTGAG 2711200 1.7329912 15.016164 9
AACTT 2759505 1.4352318 7.5377293 2
ACTTT 2668000 1.3505586 6.988861 3
TAACT 1903950 0.9902535 7.2072744 1
Produced by FastQC (version 0.10.1)