##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_14_CAGATC_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29304156	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45736737819714	34.0	31.0	34.0	31.0	34.0
2	32.614690865009045	34.0	31.0	34.0	31.0	34.0
3	32.68878929664447	34.0	31.0	34.0	31.0	34.0
4	36.047571682323834	37.0	37.0	37.0	35.0	37.0
5	36.06255085456138	37.0	37.0	37.0	35.0	37.0
6	36.05098788717887	37.0	37.0	37.0	35.0	37.0
7	36.07463347519717	37.0	37.0	37.0	35.0	37.0
8	36.07143065304457	37.0	37.0	37.0	35.0	37.0
9	37.87852405645124	39.0	39.0	39.0	37.0	39.0
10	37.857481443928975	39.0	39.0	39.0	37.0	39.0
11	37.853745659830636	39.0	39.0	39.0	37.0	39.0
12	37.82786417735423	39.0	39.0	39.0	37.0	39.0
13	37.80700764765243	39.0	39.0	39.0	37.0	39.0
14	39.358542214967734	41.0	40.0	41.0	37.0	41.0
15	39.33576547981795	41.0	40.0	41.0	37.0	41.0
16	39.32506737269622	41.0	40.0	41.0	37.0	41.0
17	39.30363829621982	41.0	40.0	41.0	37.0	41.0
18	39.28070308525521	41.0	40.0	41.0	37.0	41.0
19	39.29649023162448	41.0	40.0	41.0	37.0	41.0
20	39.275012834357014	41.0	40.0	41.0	37.0	41.0
21	39.24263903045015	41.0	40.0	41.0	37.0	41.0
22	39.20903550336	41.0	40.0	41.0	37.0	41.0
23	39.19220676411906	41.0	40.0	41.0	37.0	41.0
24	39.16984983290425	41.0	40.0	41.0	36.0	41.0
25	39.11972049288845	41.0	40.0	41.0	36.0	41.0
26	39.0883665784471	41.0	39.0	41.0	36.0	41.0
27	39.053231596228194	41.0	39.0	41.0	36.0	41.0
28	39.023716055838634	41.0	39.0	41.0	36.0	41.0
29	38.97217432230432	41.0	39.0	41.0	36.0	41.0
30	38.93411170074306	41.0	39.0	41.0	36.0	41.0
31	38.90025094051506	41.0	39.0	41.0	36.0	41.0
32	38.85710822041761	41.0	39.0	41.0	36.0	41.0
33	38.81641051187415	41.0	39.0	41.0	35.0	41.0
34	38.782161649699106	41.0	39.0	41.0	35.0	41.0
35	38.741170876922716	41.0	39.0	41.0	35.0	41.0
36	38.70199223618657	41.0	39.0	41.0	35.0	41.0
37	38.6696343003361	41.0	39.0	41.0	35.0	41.0
38	38.63330013667686	40.0	39.0	41.0	35.0	41.0
39	38.600950254291575	40.0	39.0	41.0	35.0	41.0
40	38.5436217647763	40.0	39.0	41.0	35.0	41.0
41	38.49991100238478	40.0	38.0	41.0	35.0	41.0
42	38.446005576819886	40.0	38.0	41.0	35.0	41.0
43	38.42278798270116	40.0	38.0	41.0	34.0	41.0
44	38.36942674615846	40.0	38.0	41.0	34.0	41.0
45	38.32374564891069	40.0	38.0	41.0	34.0	41.0
46	38.27372509892454	40.0	38.0	41.0	34.0	41.0
47	38.209834843904055	40.0	38.0	41.0	34.0	41.0
48	38.357869750625134	40.0	38.0	41.0	34.0	41.0
49	38.38714662179658	40.0	38.0	41.0	34.0	41.0
50	38.361595024268915	40.0	38.0	41.0	34.0	41.0
51	36.85630471664156	39.0	37.0	40.0	33.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	139268.0
3	3299.0
4	2588.0
5	1783.0
6	2064.0
7	2365.0
8	5240.0
9	8133.0
10	9253.0
11	11084.0
12	13832.0
13	17980.0
14	21576.0
15	24344.0
16	25725.0
17	26681.0
18	27338.0
19	29196.0
20	31325.0
21	33974.0
22	38474.0
23	43499.0
24	50738.0
25	59345.0
26	69238.0
27	82698.0
28	101131.0
29	122884.0
30	152617.0
31	194620.0
32	256672.0
33	349830.0
34	504097.0
35	761507.0
36	1178917.0
37	1988127.0
38	4564597.0
39	1.8330863E7
40	17254.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.486709883040117	29.195347396724515	28.876366835284177	22.44157588495119
2	21.80588446242391	29.811200730204696	30.186443283893077	18.19647152347832
3	21.501630546590334	28.4675345308259	30.541068172481022	19.489766750102746
4	21.315755490474878	27.1513841496553	31.068275318368745	20.464585041501078
5	24.23555421547751	27.437611037133987	28.990676824616784	19.336157922771726
6	21.349466350391744	27.07124539168353	31.57624534965915	20.003042908265577
7	23.542265753544175	27.46334281080657	29.455148632991317	19.539242802657935
8	21.537414109348678	27.41061209719794	31.088123947146578	19.963849846306804
9	21.39760622501655	27.42452463716986	31.289437536812482	19.88843160100111
10	21.555780829672667	28.14589942147146	30.550307683938232	19.748012064917635
11	23.690303664660007	27.363843567444867	29.07024814010082	19.875604627794306
12	20.958067999347854	29.874353042107316	29.13475702765395	20.032821930890883
13	22.52707178045234	27.64100265812875	29.56972381199865	20.262201749420257
14	22.718934598190422	27.796226810501917	29.016506546846472	20.468332044461185
15	22.25317395527267	28.45892363208155	28.673760051032737	20.614142361613037
16	22.414583795720812	27.96305526743189	28.85632873352296	20.766032203324333
17	22.352207975375745	27.981223797367104	28.90680463275755	20.759763594499603
18	22.201662187263075	27.907699457601908	28.967516333602333	20.923122021532688
19	21.932015089129077	28.129934746356223	29.160735281194487	20.777314883320205
20	21.818966837175687	28.165478399355504	29.1079873075874	20.90756745588141
21	21.861654585674366	28.202845409437327	29.06671482940733	20.86878517548098
22	21.753789128212887	28.285613886688203	29.13928011016031	20.821316874938603
23	21.753983290018258	28.337554648511087	29.03640609574395	20.87205596572671
24	21.669349228212276	28.386943915042412	29.01346648068427	20.930240376061043
25	21.696032763781997	28.494071547613903	28.897676990770947	20.91221869783315
26	21.708334671397512	28.470128780786112	28.906622470749916	20.914914077066456
27	21.7144615314528	28.41816095086409	28.82505399923679	21.042323518446327
28	21.698782447952787	28.424370278305062	28.868805453201755	21.008041820540395
29	21.699111056620286	28.489556181833937	28.822396809485873	20.98893595205991
30	21.729829021841887	28.459584763848955	28.714217602530244	21.096368611778914
31	21.817059642688378	28.40989495338543	28.68466477707431	21.088380626851887
32	21.798179152669956	28.48568962328176	28.607154726716903	21.108976497331383
33	21.80184003737981	28.454432300947314	28.571124094102736	21.172603567570143
34	21.835148565801795	28.449198216988687	28.533472883972998	21.182180333236523
35	21.758576214512285	28.473918299070206	28.56098920178039	21.206516284637118
36	21.7843314278107	28.46157577451972	28.547885743891044	21.206207053778538
37	21.834646657146166	28.417191556953064	28.55627238401764	21.191889401883127
38	21.7742218616429	28.458930078098255	28.579901466437896	21.18694659382095
39	21.75796444654276	28.390118051116627	28.58088804613153	21.271029456209085
40	21.800205627415174	28.414104566324728	28.531956983661754	21.25373282259834
41	21.789277629593837	28.39628443446281	28.511769526761654	21.3026684091817
42	21.761531747040866	28.369723547621632	28.513716060752227	21.355028644585275
43	21.812194569597846	28.435700595241382	28.461321557937048	21.29078327722372
44	21.830238705857248	28.448210343420666	28.40339854593609	21.318152404785994
45	21.813183810245697	28.415786340590838	28.455642589117076	21.315387260046396
46	21.74200916226168	28.479764199225965	28.421858968876602	21.356367669635752
47	21.815362016531623	28.449615026617153	28.388487600595614	21.346535356255607
48	21.77129015140471	28.441045241338962	28.406925212501015	21.380739394755313
49	21.777233106605205	28.513975319790617	28.338001992428115	21.370789581176066
50	21.76662655044175	28.4742424315238	28.401226554149005	21.357904463885443
51	21.818016604839357	28.424205933390123	28.383751437474626	21.374026024295894
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	41.928285767991305
2	40.002355985902234
3	40.99139729669308
4	41.78034053197596
5	43.57171213824923
6	41.35250925865732
7	43.08150855620212
8	41.50126395565548
9	41.286037826017655
10	41.3037928945903
11	43.56590829245432
12	40.99088993023874
13	42.789273529872595
14	43.18726664265161
15	42.867316316885706
16	43.180615999045145
17	43.111971569875344
18	43.12478420879577
19	42.70932997244928
20	42.72653429305709
21	42.730439761155345
22	42.57510600315149
23	42.62603925574497
24	42.59958960427332
25	42.60825146161515
26	42.62324874846397
27	42.75678504989912
28	42.70682426849318
29	42.68804700868019
30	42.8261976336208
31	42.905440269540264
32	42.907155650001336
33	42.97444360494996
34	43.01732889903832
35	42.96509249914941
36	42.99053848158923
37	43.02653605902929
38	42.96116845546385
39	43.02899390275184
40	43.05393845001352
41	43.09194603877554
42	43.11656039162614
43	43.10297784682157
44	43.148391110643246
45	43.12857107029209
46	43.09837683189743
47	43.16189737278723
48	43.15202954616002
49	43.14802268778127
50	43.12453101432719
51	43.19204262913525
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	146559.0
1	76962.0
2	7365.0
3	7635.0
4	7905.0
5	12423.0
6	16941.0
7	21026.5
8	25112.0
9	22151.5
10	19191.0
11	21914.0
12	24637.0
13	32448.5
14	40260.0
15	59145.0
16	78030.0
17	124643.5
18	171257.0
19	213431.0
20	255605.0
21	295262.0
22	334919.0
23	398615.5
24	462312.0
25	553331.0
26	767354.0
27	890358.0
28	968488.0
29	1046618.0
30	1105342.5
31	1164067.0
32	1252841.5
33	1341616.0
34	1426451.5
35	1511287.0
36	1675555.5
37	1839824.0
38	1902259.5
39	1964695.0
40	2033742.5
41	2102790.0
42	2094484.5
43	2086179.0
44	2075732.5
45	2065286.0
46	2000806.5
47	1936327.0
48	1866776.0
49	1797225.0
50	1700879.0
51	1604533.0
52	1487369.0
53	1370205.0
54	1255869.5
55	1141534.0
56	1024466.5
57	907399.0
58	799913.5
59	692428.0
60	599501.0
61	506574.0
62	427370.5
63	348167.0
64	291000.5
65	233834.0
66	193935.5
67	154037.0
68	130974.0
69	107911.0
70	93032.5
71	78154.0
72	65079.5
73	52005.0
74	42886.5
75	28118.5
76	22469.0
77	20176.0
78	17883.0
79	17451.5
80	17020.0
81	14424.0
82	11828.0
83	10171.0
84	8514.0
85	7126.5
86	5739.0
87	6055.5
88	6372.0
89	4139.5
90	1907.0
91	1260.5
92	614.0
93	440.5
94	267.0
95	209.5
96	152.0
97	114.5
98	77.0
99	63.5
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8222144326559004
2	0.5216939194563393
3	0.508125878117766
4	0.5080883407800586
5	0.4947830608054366
6	0.5269252593386412
7	0.49785429752694466
8	0.4917868987593432
9	0.4874803423787397
10	0.5137087039804183
11	0.5064435228914288
12	0.4947898857759288
13	0.5112312396917352
14	0.4747517724107119
15	0.5209977724661308
16	0.4757106807648718
17	0.5090267742227417
18	0.49147977508719237
19	0.5116066130688084
20	0.5088015501964976
21	0.5220795302891508
22	0.5475230202842218
23	0.48806387735582624
24	0.5196976155873589
25	0.5285803146830095
26	0.5377121252016267
27	0.5246491316794791
28	0.5205677993251197
29	0.5794126949092135
30	0.5776143151845082
31	0.5662985141083743
32	0.5768430935188852
33	0.5718438026333192
34	0.5627631793933938
35	0.5659060783050704
36	0.5946767414151085
37	0.5556481476552336
38	0.5659982154067157
39	0.5572759031176329
40	0.5665339755903566
41	0.5671413979641658
42	0.5635412260295093
43	0.5328561586963979
44	0.5461512012152815
45	0.5354428225129568
46	0.5282629535551203
47	0.5798972678141625
48	0.5859476041555335
49	0.6001810801171001
50	0.587411560326119
51	0.5554570484814508
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9304156E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.42452333013527
#Duplication Level	Relative count
1	100.0
2	10.868197150480793
3	3.1238987061370387
4	1.4392337511956905
5	0.9018023460705835
6	0.6192418063736596
7	0.42793132960781355
8	0.36940542717615665
9	0.30584503851381967
10++	7.806600211448422
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	132767	0.4530654286716191	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	5295890	3.2946596	24.553665	4
TTTTT	8638840	3.0448406	3.6739838	17
TGTTT	5134380	2.4042432	18.670685	6
GTTTG	3780510	2.351917	23.887064	7
CTTTG	3274815	2.1435437	25.183403	1
GTGTT	3370600	2.0969052	23.770409	5
GAGGG	1856320	2.094354	7.9364734	11
TTTGA	4225540	2.0329928	18.68961	8
TTGTG	3132705	1.9489069	23.772936	3
TTTGT	4031680	1.8878889	18.291512	2
TGAGG	2127795	1.8069487	9.390667	10
TTGAG	2711200	1.7329912	15.016164	9
AACTT	2759505	1.4352318	7.5377293	2
ACTTT	2668000	1.3505586	6.988861	3
TAACT	1903950	0.9902535	7.2072744	1
>>END_MODULE