FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_D_14_CAGATC_L005_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_D_14_CAGATC_L005_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29304156
Filtered Sequences 0
Sequence length 51
%GC 42

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[OK] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8522565 3.0176227 3.5713365 13
TGTGT 4618195 2.9421418 24.54283 4
TGTTT 4536130 2.1544108 18.35391 6
GTTTG 3149780 2.00665 23.685936 7
GAGGG 1705620 1.9990124 8.006213 11
CTTTG 2962300 1.9578373 24.536127 1
TTTGT 3734610 1.7737328 18.036594 2
TTTGA 3626795 1.7612098 18.331625 8
GTGTT 2741095 1.7462863 23.598566 5
TGAGG 1960450 1.712934 9.567398 10
TTGTG 2574835 1.6403657 23.598188 3
TTGAG 2338130 1.5230182 15.00369 9