##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_D_14_CAGATC_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29304156	
Filtered Sequences	0	
Sequence length	51	
%GC	42	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.89528434123815	34.0	33.0	34.0	31.0	34.0
2	33.10711941336922	34.0	33.0	34.0	31.0	34.0
3	33.163932617612325	34.0	33.0	34.0	31.0	34.0
4	36.48959604910648	37.0	37.0	37.0	35.0	37.0
5	36.4353428230453	37.0	37.0	37.0	35.0	37.0
6	36.40579547829325	37.0	37.0	37.0	35.0	37.0
7	36.4039798313932	37.0	37.0	37.0	35.0	37.0
8	36.397870117808544	37.0	37.0	37.0	35.0	37.0
9	38.220331443772004	39.0	39.0	39.0	37.0	39.0
10	38.206364005160225	39.0	39.0	39.0	37.0	39.0
11	38.19343109557566	39.0	39.0	39.0	37.0	39.0
12	38.15575835045377	39.0	39.0	39.0	37.0	39.0
13	38.155128337427634	39.0	39.0	39.0	37.0	39.0
14	39.7133482363389	41.0	40.0	41.0	38.0	41.0
15	39.67045438879045	41.0	40.0	41.0	37.0	41.0
16	39.65764798003396	41.0	40.0	41.0	37.0	41.0
17	39.65101212947406	41.0	40.0	41.0	37.0	41.0
18	39.62662876214555	41.0	40.0	41.0	37.0	41.0
19	39.615641481024056	41.0	40.0	41.0	37.0	41.0
20	39.559411538759214	41.0	40.0	41.0	37.0	41.0
21	39.55292375593414	41.0	40.0	41.0	37.0	41.0
22	39.52184778841609	41.0	39.0	41.0	37.0	41.0
23	39.476703713971496	41.0	39.0	41.0	37.0	41.0
24	39.472448686118106	41.0	39.0	41.0	37.0	41.0
25	39.430474366844074	41.0	39.0	41.0	37.0	41.0
26	39.36821237915878	41.0	39.0	41.0	36.0	41.0
27	39.29395943019141	41.0	39.0	41.0	36.0	41.0
28	39.24938036092901	40.0	39.0	41.0	36.0	41.0
29	39.19134825108084	40.0	39.0	41.0	36.0	41.0
30	39.143688833761324	40.0	39.0	41.0	36.0	41.0
31	39.088336139078706	40.0	39.0	41.0	36.0	41.0
32	39.03624455179668	40.0	39.0	41.0	36.0	41.0
33	38.96080204459736	40.0	39.0	41.0	35.0	41.0
34	35.763356228379344	40.0	38.0	41.0	24.0	41.0
35	38.82841594209368	40.0	38.0	41.0	35.0	41.0
36	38.77711383327334	40.0	38.0	41.0	35.0	41.0
37	38.92170346076509	40.0	38.0	41.0	35.0	41.0
38	38.95220265002684	40.0	39.0	41.0	35.0	41.0
39	38.9316594888452	40.0	39.0	41.0	35.0	41.0
40	38.89509180882057	40.0	39.0	41.0	35.0	41.0
41	38.8586130922863	40.0	38.0	41.0	35.0	41.0
42	38.806320031875345	40.0	38.0	41.0	35.0	41.0
43	38.76491501751492	40.0	38.0	41.0	35.0	41.0
44	38.71615759894262	40.0	38.0	41.0	35.0	41.0
45	38.66222995809878	40.0	38.0	41.0	35.0	41.0
46	38.592067555195925	40.0	38.0	41.0	35.0	41.0
47	38.53958438523191	40.0	38.0	41.0	35.0	41.0
48	38.48234079834956	40.0	38.0	41.0	34.0	41.0
49	38.4396464446886	40.0	38.0	41.0	34.0	41.0
50	38.559649081857195	40.0	38.0	41.0	35.0	41.0
51	38.280872003274894	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	9.0
10	9.0
11	13.0
12	39.0
13	99.0
14	229.0
15	367.0
16	599.0
17	971.0
18	1576.0
19	2527.0
20	4432.0
21	7218.0
22	11787.0
23	18530.0
24	27499.0
25	39156.0
26	54869.0
27	74647.0
28	99661.0
29	132431.0
30	175223.0
31	229894.0
32	304468.0
33	411525.0
34	591731.0
35	884984.0
36	1377799.0
37	2433124.0
38	6255119.0
39	1.6153262E7
40	10353.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.976165016185227	29.4839174081438	28.092415326834196	22.447502248836777
2	21.745824964355272	28.72570453843819	30.77421604628215	18.754254450924396
3	20.631624956037527	27.5784361146873	31.930824960187216	19.859113969087957
4	21.167394254517518	27.48049626851309	31.603651443605095	19.7484580333643
5	23.7552641436542	28.0670153567674	28.80477778078456	19.372942718793848
6	21.64726054556903	27.682264590729044	30.459638557752694	20.210836305949233
7	23.64568698037234	28.218652671655175	28.31809590421236	19.817564443760126
8	20.955265340108316	27.954777350455057	30.739536375704258	20.35042093373237
9	20.669493461579346	27.868221147931717	31.185658523151798	20.276626867337143
10	20.981999959323176	28.03442624315814	30.900084615984163	20.08348918153452
11	23.282332376335972	27.89259311887365	28.69487181272172	20.13020269206866
12	20.89990238927202	30.321149669009408	28.51670595802179	20.262241983696782
13	22.289060295747813	28.140578421709193	29.087362215789458	20.482999066753536
14	21.871247254513158	28.189516722651923	29.293120361610843	20.64611566122407
15	21.971371569275018	28.21611037014681	29.05242519183968	20.760092868738482
16	21.87657614162305	28.16658838425512	29.062928821427242	20.89390665269459
17	21.950743095962224	28.272419789193044	28.933281681956647	20.843555432888085
18	21.785650472240185	28.191513176492784	29.053268075695478	20.96956827557156
19	21.58806004784444	28.423153706237862	29.187862800435443	20.80092344548225
20	21.464013500337632	28.420112150645117	29.19682450502925	20.919049843988
21	21.56543321570668	28.37346594405354	29.100878186798695	20.960222653441086
22	21.534501795581487	28.41914982980571	29.126083685877184	20.920264688735617
23	21.60232835233337	28.459734516837816	28.994692766445823	20.94324436438299
24	21.500179018976013	28.483638975986885	28.985062733081275	21.031119271955827
25	21.522628230072335	28.568644451299036	28.93823627661924	20.97049104200939
26	21.56831611188529	28.537614255124765	28.902354328171064	20.991715304818882
27	21.584245593014177	28.480120703698137	28.80116049068262	21.134473212605066
28	21.560107535728505	28.49469192404713	28.87980840646952	21.06539213375484
29	21.55688087382554	28.57339075044509	28.824331265503773	21.045397110225593
30	21.56421089213421	28.522769944304144	28.769850938549467	21.14316822501218
31	21.660337189032163	28.49172656602019	28.707508245588098	21.140427999359545
32	21.632501405705494	28.570566176915474	28.65647656304947	21.140455854329566
33	21.638148993057275	28.51794205920342	28.618358213284512	21.225550734454792
34	21.676318711521443	28.534750064261015	28.585357361143547	21.20357386307399
35	21.597761807851857	28.56388608538129	28.599132130968137	21.23921997579872
36	21.65976893076738	28.539327537553845	28.577082475958143	21.22382105572063
37	21.693798240358003	28.53303514322046	28.562840959046998	21.210325657374536
38	21.639799215613685	28.525144271163548	28.61775040965001	21.21730610357275
39	21.60696410079098	28.478305128773364	28.618751777508706	21.295978992926955
40	21.651313354381983	28.524307452646507	28.572882839674673	21.251496353296837
41	21.653446896614703	28.478716678682158	28.57240065277456	21.295435771928577
42	21.65128735307252	28.436512701541034	28.531775880234022	21.380424065152425
43	21.678616115144692	28.520045776406448	28.49484384099336	21.3064942674555
44	21.671649321050634	28.50243815976831	28.478819948007857	21.347092571173196
45	21.689608125420232	28.457627100713832	28.52180992703708	21.33095484682885
46	21.616508703851338	28.5177794247191	28.51625745619546	21.3494544152341
47	21.68436198602998	28.529236299450655	28.457095007351658	21.32930670716771
48	21.655805408625316	28.503806763791456	28.459038369847605	21.381349457735617
49	21.665550771196386	28.558157264138256	28.40830990635351	21.367982058311853
50	21.645256711636478	28.518838842577267	28.443981294075982	21.391923151710273
51	21.706351140090845	28.466023727146418	28.44209879308587	21.38552633967687
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.42366726502201
2	40.500079415279664
3	40.490738925125484
4	40.91585228788182
5	43.128206862448046
6	41.85809685151826
7	43.463251424132466
8	41.305686273840685
9	40.946120328916486
10	41.0654891408577
11	43.41253506840464
12	41.162144372968804
13	42.772059362501345
14	42.51736291573723
15	42.731464438013504
16	42.77048279431764
17	42.79429852885031
18	42.755218747811746
19	42.38898349332669
20	42.383063344325635
21	42.52565586914777
22	42.454766484317105
23	42.54557271671636
24	42.531298290931844
25	42.49311927208173
26	42.56003141670417
27	42.71871880561925
28	42.62549966948335
29	42.602277984051135
30	42.70737911714639
31	42.80076518839171
32	42.772957260035064
33	42.86369972751206
34	42.87989257459544
35	42.83698178365057
36	42.88358998648801
37	42.90412389773254
38	42.85710531918644
39	42.90294309371794
40	42.90280970767883
41	42.948882668543284
42	43.031711418224944
43	42.98511038260019
44	43.01874189222383
45	43.02056297224908
46	42.96596311908544
47	43.013668693197694
48	43.037154866360936
49	43.03353282950824
50	43.03717986334675
51	43.09187747976772
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15654.0
1	9645.0
2	3636.0
3	4844.5
4	6053.0
5	7999.0
6	9945.0
7	14767.5
8	19590.0
9	17786.5
10	15983.0
11	19336.5
12	22690.0
13	31442.0
14	40194.0
15	62029.5
16	83865.0
17	135087.0
18	186309.0
19	231352.0
20	276395.0
21	318866.0
22	361337.0
23	428301.0
24	495265.0
25	594303.0
26	818519.0
27	943697.0
28	1014811.0
29	1085925.0
30	1139167.5
31	1192410.0
32	1278855.5
33	1365301.0
34	1449680.5
35	1534060.0
36	1688459.5
37	1842859.0
38	1899801.5
39	1956744.0
40	2015310.0
41	2073876.0
42	2065668.0
43	2057460.0
44	2046544.0
45	2035628.0
46	1975234.5
47	1914841.0
48	1848146.5
49	1781452.0
50	1685573.5
51	1589695.0
52	1478070.5
53	1366446.0
54	1250325.0
55	1134204.0
56	1020385.5
57	906567.0
58	797910.0
59	689253.0
60	597818.0
61	506383.0
62	426457.0
63	346531.0
64	289254.0
65	231977.0
66	192363.5
67	152750.0
68	130663.0
69	108576.0
70	93215.0
71	77854.0
72	64752.5
73	51651.0
74	42739.0
75	27978.5
76	22130.0
77	20327.0
78	18524.0
79	18104.5
80	17685.0
81	15144.5
82	12604.0
83	10675.0
84	8746.0
85	7346.0
86	5946.0
87	6104.5
88	6263.0
89	3926.5
90	1590.0
91	955.0
92	320.0
93	209.5
94	99.0
95	58.5
96	18.0
97	12.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.26974672125005067
2	0.008497088262838895
3	0.002573013875574509
4	6.824970492240077E-5
5	2.3887396722840272E-5
6	0.0
7	0.0
8	8.735962230067298E-4
9	2.0816160001332237E-4
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0020816160001332234
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0029210873706787526
20	0.0
21	4.7774793445680533E-4
22	0.0
23	0.0
24	0.0
25	7.063844459468479E-4
26	0.0
27	0.0
28	1.1943698361420133E-4
29	0.0
30	0.0
31	0.0
32	0.001245557114833814
33	3.617234360887241E-4
34	8.462830323453097
35	0.00538490171837742
36	0.006923932564377558
37	0.003924358033038044
38	0.0016482303738759787
39	0.0017130675935522592
40	0.0042656065576500484
41	0.0010851703082661722
42	2.525239082128828E-4
43	0.0029040249444481528
44	0.001699417652567779
45	0.001252382085326054
46	6.824970492240076E-6
47	0.0018768668853660212
48	0.0
49	0.0011465950426963328
50	0.001699417652567779
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9304156E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	17.295136037479296
#Duplication Level	Relative count
1	100.0
2	3.1269329687143927
3	0.9283675706177904
4	0.5821966120823431
5	0.3977167909192521
6	0.3304359149656542
7	0.22734425019804452
8	0.1850224088723942
9	0.1535523217328081
10++	2.5859730225390933
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	8522565	3.0176227	3.5713365	13
TGTGT	4618195	2.9421418	24.54283	4
TGTTT	4536130	2.1544108	18.35391	6
GTTTG	3149780	2.00665	23.685936	7
GAGGG	1705620	1.9990124	8.006213	11
CTTTG	2962300	1.9578373	24.536127	1
TTTGT	3734610	1.7737328	18.036594	2
TTTGA	3626795	1.7612098	18.331625	8
GTGTT	2741095	1.7462863	23.598566	5
TGAGG	1960450	1.712934	9.567398	10
TTGTG	2574835	1.6403657	23.598188	3
TTGAG	2338130	1.5230182	15.00369	9
>>END_MODULE