FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_9_ACTTGA_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_9_ACTTGA_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24153543
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 106573 0.44123133405314496 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 6615995 4.547103 44.014675 4
TTTTT 9867860 3.977284 5.1981344 17
AAAAA 5865560 3.2969513 3.4710255 44
GTTTG 4686585 3.2210402 43.30671 7
TGTTT 5830860 3.068911 33.76014 6
GTGTT 4449350 3.0579908 43.30685 5
CTTTG 3830715 2.8823526 47.414192 1
TTGTG 4161715 2.8603027 43.309986 3
TTTGA 5011765 2.819228 35.59454 8
GGGGG 1644975 2.5174623 6.506168 13
GAGGG 1893140 2.3712988 13.600996 11
TTTGT 4504165 2.3706417 33.40008 2
TTGAG 3164980 2.3248692 27.764343 9
GAGAG 2193945 2.2492056 5.0451655 11
TGAGG 2288580 2.1952379 16.98825 10
AGGGG 1449970 1.8161955 7.817147 12
ACTTT 2496390 1.537374 14.940065 3
TGAGC 1450775 1.5235025 6.20474 10
TGAGA 1935330 1.519394 5.254938 10
AACTT 2286240 1.5047932 16.444496 2
GAGTG 1423980 1.3659017 6.8772187 11
TGAGT 1857440 1.3644018 8.353156 10
TAACT 1767540 1.1633871 16.272804 1
TTGAT 2022585 1.1377486 8.336813 9
TTGAC 1254575 1.0089085 6.6996017 9
GTAAC 840320 0.7222502 5.4154806 1
Produced by FastQC (version 0.10.1)