##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_9_ACTTGA_L003_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	24153543	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.420412028164975	34.0	31.0	34.0	31.0	34.0
2	32.58105040738744	34.0	31.0	34.0	31.0	34.0
3	32.65091278741177	34.0	31.0	34.0	31.0	34.0
4	35.99680316051355	37.0	37.0	37.0	35.0	37.0
5	36.02128934044997	37.0	37.0	37.0	35.0	37.0
6	36.00982058822592	37.0	37.0	37.0	35.0	37.0
7	36.04531509104068	37.0	37.0	37.0	35.0	37.0
8	36.052070331876365	37.0	37.0	37.0	35.0	37.0
9	37.83977584572168	39.0	39.0	39.0	37.0	39.0
10	37.82455215783457	39.0	39.0	39.0	37.0	39.0
11	37.80062920789716	39.0	39.0	39.0	37.0	39.0
12	37.77164025170138	39.0	39.0	39.0	37.0	39.0
13	37.78047970022452	39.0	39.0	39.0	37.0	39.0
14	39.31565319423324	41.0	40.0	41.0	37.0	41.0
15	39.27098148706382	41.0	40.0	41.0	37.0	41.0
16	39.26275768321028	41.0	40.0	41.0	37.0	41.0
17	39.23817391924655	41.0	40.0	41.0	37.0	41.0
18	39.21020448221613	41.0	40.0	41.0	37.0	41.0
19	39.21895711117826	41.0	40.0	41.0	37.0	41.0
20	39.19143021791876	41.0	40.0	41.0	37.0	41.0
21	39.14923777435054	41.0	40.0	41.0	37.0	41.0
22	39.126301967375966	41.0	40.0	41.0	36.0	41.0
23	39.09444067895132	41.0	40.0	41.0	36.0	41.0
24	39.09272465741361	41.0	40.0	41.0	36.0	41.0
25	39.038979001962566	41.0	39.0	41.0	36.0	41.0
26	38.991755660856874	41.0	39.0	41.0	36.0	41.0
27	38.94995239414772	41.0	39.0	41.0	36.0	41.0
28	38.931825695302756	41.0	39.0	41.0	36.0	41.0
29	38.88208798187496	41.0	39.0	41.0	36.0	41.0
30	38.83819802337073	41.0	39.0	41.0	35.0	41.0
31	38.79668382398392	41.0	39.0	41.0	35.0	41.0
32	38.7600477495165	41.0	39.0	41.0	35.0	41.0
33	38.70470410904106	41.0	39.0	41.0	35.0	41.0
34	38.66608853202199	41.0	39.0	41.0	35.0	41.0
35	38.63105255407043	41.0	39.0	41.0	35.0	41.0
36	38.58320781344584	40.0	39.0	41.0	35.0	41.0
37	38.5275365605783	40.0	39.0	41.0	35.0	41.0
38	38.482041495941196	40.0	38.0	41.0	35.0	41.0
39	38.44304626447557	40.0	38.0	41.0	35.0	41.0
40	38.401285848622706	40.0	38.0	41.0	34.0	41.0
41	38.35222869787675	40.0	38.0	41.0	34.0	41.0
42	38.27399408856912	40.0	38.0	41.0	34.0	41.0
43	38.24138454553024	40.0	38.0	41.0	34.0	41.0
44	38.19263041451103	40.0	38.0	41.0	34.0	41.0
45	38.14769063072858	40.0	38.0	41.0	34.0	41.0
46	38.08862443079262	40.0	38.0	41.0	33.0	41.0
47	38.01899684033933	40.0	38.0	41.0	33.0	41.0
48	38.200510252264024	40.0	38.0	41.0	34.0	41.0
49	38.26195788336312	40.0	38.0	41.0	34.0	41.0
50	38.240395208272346	40.0	38.0	41.0	34.0	41.0
51	36.73184501337961	39.0	37.0	40.0	32.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113034.0
3	2908.0
4	2091.0
5	1435.0
6	1487.0
7	2208.0
8	6571.0
9	11023.0
10	9753.0
11	10150.0
12	12842.0
13	17050.0
14	21000.0
15	23756.0
16	25549.0
17	26829.0
18	27989.0
19	29455.0
20	30782.0
21	33311.0
22	37258.0
23	41767.0
24	47851.0
25	54656.0
26	70651.0
27	78393.0
28	87548.0
29	105882.0
30	130022.0
31	163498.0
32	212198.0
33	284795.0
34	412088.0
35	638970.0
36	986605.0
37	1641217.0
38	3892992.0
39	1.4846639E7
40	11290.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.739998075338598	26.47171363543281	31.054119516463263	23.734168772765333
2	20.801536421186892	28.522262769816624	33.033559407537695	17.64264140145879
3	21.850772191639713	27.60944489751862	32.080770632531525	18.45901227831014
4	20.76939273946164	25.173309187971615	33.520226438546516	20.537071634020226
5	27.260734137298165	25.12319094691509	29.097486992549303	18.518587923237437
6	20.556982354322578	24.78081820820732	35.7159994029561	18.946200034514
7	25.526763015177362	25.278724498408668	30.792152494234898	18.402359992179072
8	22.236049669655458	25.378004308306483	33.67322064379664	18.71272537824142
9	22.1536671922748	25.66638909209315	33.45399103667405	18.725952678958002
10	22.14970622427002	27.273529338078845	31.895976482293758	18.680787955357374
11	26.63167891629183	25.451330367106657	28.97562288450109	18.94136783210042
12	20.932374637480194	30.739994020566453	29.174429498450046	19.15320184350331
13	24.081271731911645	25.961900806775112	30.193999703721953	19.762827757591293
14	24.763865296339844	26.498597651764737	28.709294513269356	20.028242538626056
15	23.61355368943381	28.227702728496386	27.931643155400494	20.227100426669313
16	24.027932323912424	26.953531572214818	28.45214960380243	20.566386500070326
17	23.750414492109005	27.023508028162823	28.68880498429258	20.537272495435595
18	23.361990120422256	27.00751927006192	28.88619868122214	20.744291928293684
19	22.967448843119705	27.4165521070664	29.16181575270558	20.45418329710832
20	22.65541408701296	27.495476042815724	29.2125280632669	20.636581806904413
21	22.629145363292913	27.596553969925296	29.129447688994365	20.64485297778743
22	22.360560904124473	27.79991143023024	29.26817020984607	20.571357455799223
23	22.376954178464032	27.88414117230712	29.118936723667314	20.619967925561536
24	22.208438268312037	28.000553633056718	29.05036741651404	20.740640682117206
25	22.249818199598632	28.069103919086075	28.94454645611458	20.736531425200706
26	22.16636624149272	28.08672974034399	28.952949067995153	20.793954950168132
27	22.21472966827433	28.077516008726455	28.80529361215124	20.902460710847976
28	22.140092914032056	28.109132219949128	28.831543145718076	20.91923172030074
29	22.2357538771327	28.088201100834215	28.717592327043846	20.958452694989237
30	22.179498361835112	28.07466562976425	28.697164958535275	21.048671049865362
31	22.286431504131087	28.05397197599655	28.6169315960218	21.04266492385057
32	22.25900962708205	28.095533999430337	28.576432323410085	21.069024050077527
33	22.249026497610505	28.07012213748845	28.519982540134137	21.160868824766908
34	22.227944696294333	28.099652495977022	28.51871957800182	21.153683229726823
35	22.28280977836382	28.08868676576099	28.46461730693709	21.163886148938108
36	22.21485341863905	28.10286831303793	28.476231356144226	21.206046912178795
37	22.244905977379435	28.082122167309887	28.46288572881224	21.21008612649844
38	22.187671882345576	28.081829647199207	28.513216878396864	21.217281592058352
39	22.17663563677036	28.021726682655697	28.475582132467846	21.3260555481061
40	22.183284259794043	28.039202794944735	28.462143745245918	21.315369200015315
41	22.27124126100718	28.004950086585325	28.418206256021016	21.30560239638648
42	22.200870766881646	28.018577958933456	28.400097942642432	21.380453331542466
43	22.25597498622222	28.054015131397946	28.37253013851187	21.317479743867956
44	22.23801856979037	28.03699325180587	28.36156796358657	21.363420214817193
45	22.239697450152672	28.01143982904422	28.343891291247253	21.404971429555854
46	22.17098131449761	28.092562376903434	28.319245239364438	21.417211069234522
47	22.23176459356476	28.028846742089875	28.32077921461771	21.41860944972766
48	22.158250477879715	28.021628155724688	28.28967965102425	21.53044171537135
49	22.16357120396632	28.064997120862788	28.26601854343335	21.50541313173754
50	22.162998300319224	28.066016818345247	28.27090328716906	21.500081594166474
51	22.198506575942147	28.04374548413355	28.256037762599597	21.501710177324703
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.47416684810393
2	38.44417782264568
3	40.309784469949854
4	41.306464373481866
5	45.7793220605356
6	39.50318238883658
7	43.92912300735644
8	40.94877504789688
9	40.879619871232805
10	40.830494179627394
11	45.57304674839225
12	40.085576480983505
13	43.84409948950294
14	44.792107834965904
15	43.84065411610313
16	44.594318823982746
17	44.287686987544596
18	44.10628204871594
19	43.42163214022803
20	43.29199589391738
21	43.27399834108034
22	42.93191835992369
23	42.99692210402557
24	42.94907895042924
25	42.98634962479934
26	42.96032119166085
27	43.117190379122306
28	43.059324634332796
29	43.19420657212194
30	43.22816941170047
31	43.32909642798165
32	43.328033677159574
33	43.40989532237741
34	43.38162792602115
35	43.446695927301924
36	43.420900330817844
37	43.45499210387787
38	43.40495347440393
39	43.50269118487646
40	43.498653459809354
41	43.57684365739366
42	43.58132409842411
43	43.57345473009018
44	43.60143878460756
45	43.64466887970852
46	43.588192383732135
47	43.65037404329242
48	43.68869219325106
49	43.66898433570386
50	43.6630798944857
51	43.70021675326685
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128111.0
1	68095.0
2	8079.0
3	8648.5
4	9218.0
5	23365.0
6	37512.0
7	48617.5
8	59723.0
9	44583.0
10	29443.0
11	28298.0
12	27153.0
13	33061.5
14	38970.0
15	51896.5
16	64823.0
17	88268.0
18	111713.0
19	141377.0
20	171041.0
21	207989.0
22	244937.0
23	295366.5
24	345796.0
25	408909.0
26	549503.0
27	626984.0
28	698871.0
29	770758.0
30	842802.0
31	914846.0
32	997122.0
33	1079398.0
34	1157533.5
35	1235669.0
36	1331634.5
37	1427600.0
38	1498766.5
39	1569933.0
40	1630636.0
41	1691339.0
42	1717395.5
43	1743452.0
44	1748918.5
45	1754385.0
46	1721526.5
47	1688668.0
48	1629122.5
49	1569577.0
50	1486084.5
51	1402592.0
52	1299389.0
53	1196186.0
54	1089928.0
55	983670.0
56	880458.5
57	777247.0
58	683228.5
59	589210.0
60	509928.5
61	430647.0
62	363666.0
63	296685.0
64	249130.0
65	201575.0
66	166991.0
67	132407.0
68	112250.0
69	92093.0
70	79267.5
71	66442.0
72	55723.0
73	45004.0
74	37988.5
75	26143.5
76	21314.0
77	19166.0
78	17018.0
79	15886.5
80	14755.0
81	12799.5
82	10844.0
83	9538.5
84	8233.0
85	6920.0
86	5607.0
87	5654.0
88	5701.0
89	3980.0
90	2259.0
91	1592.5
92	926.0
93	719.0
94	512.0
95	395.0
96	278.0
97	213.5
98	149.0
99	107.5
100	66.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8333270195598218
2	0.5810162095059925
3	0.5788591760637353
4	0.5663102924486069
5	0.5788219144495695
6	0.5320503083129461
7	0.49923110659169134
8	0.47363237765987376
9	0.4933479117328667
10	0.5161520196022588
11	0.5306964696649266
12	0.5154978712646836
13	0.5053627122116205
14	0.5038970887211041
15	0.5480106997139095
16	0.5075984090615608
17	0.5139411638284288
18	0.5065675044029773
19	0.5157255811290294
20	0.5166985232766886
21	0.5510206101026255
22	0.564385109050047
23	0.5126204466152233
24	0.5100121336236262
25	0.5095235924601207
26	0.5351513026474004
27	0.5308082545074236
28	0.4795942359263815
29	0.5349815552939791
30	0.5092213593674435
31	0.5175182787883335
32	0.532899045080053
33	0.5211119544656451
34	0.5034085475575985
35	0.5105089551458352
36	0.5271690368572428
37	0.5239769585770502
38	0.5195055648771694
39	0.48637170952518227
40	0.5033754261227845
41	0.4969208865134196
42	0.5605968449432036
43	0.5129930627568801
44	0.5195304059532798
45	0.505370992570324
46	0.5076398108550784
47	0.5176921663211066
48	0.5652007243823401
49	0.5268295421504
50	0.5220352144610834
51	0.5092172191880918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4153543E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	20.386683390212838
#Duplication Level	Relative count
1	100.0
2	10.670908569090699
3	2.9274011834174996
4	1.3049345700799326
5	0.7993258550466222
6	0.5275917026434542
7	0.39691505407204314
8	0.3187533203470869
9	0.2802830920293351
10++	4.901595598517369
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	106573	0.44123133405314496	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	6615995	4.547103	44.014675	4
TTTTT	9867860	3.977284	5.1981344	17
AAAAA	5865560	3.2969513	3.4710255	44
GTTTG	4686585	3.2210402	43.30671	7
TGTTT	5830860	3.068911	33.76014	6
GTGTT	4449350	3.0579908	43.30685	5
CTTTG	3830715	2.8823526	47.414192	1
TTGTG	4161715	2.8603027	43.309986	3
TTTGA	5011765	2.819228	35.59454	8
GGGGG	1644975	2.5174623	6.506168	13
GAGGG	1893140	2.3712988	13.600996	11
TTTGT	4504165	2.3706417	33.40008	2
TTGAG	3164980	2.3248692	27.764343	9
GAGAG	2193945	2.2492056	5.0451655	11
TGAGG	2288580	2.1952379	16.98825	10
AGGGG	1449970	1.8161955	7.817147	12
ACTTT	2496390	1.537374	14.940065	3
TGAGC	1450775	1.5235025	6.20474	10
TGAGA	1935330	1.519394	5.254938	10
AACTT	2286240	1.5047932	16.444496	2
GAGTG	1423980	1.3659017	6.8772187	11
TGAGT	1857440	1.3644018	8.353156	10
TAACT	1767540	1.1633871	16.272804	1
TTGAT	2022585	1.1377486	8.336813	9
TTGAC	1254575	1.0089085	6.6996017	9
GTAAC	840320	0.7222502	5.4154806	1
>>END_MODULE