FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_9_ACTTGA_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_9_ACTTGA_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 24153543
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27739 0.11484443503795695 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9264165 3.8796535 4.9007897 16
TGTGT 5334975 3.8551264 46.084595 4
AAAAA 5846790 3.119271 3.2369685 46
TGTTT 4787910 2.6338584 35.095196 6
GTTTG 3613750 2.6113455 45.171577 7
CTTTG 3310140 2.5401418 48.03999 1
GTGTT 3365035 2.4316206 45.22509 5
TTTGA 3975460 2.2954278 36.28045 8
TTGTG 3105835 2.2443192 45.19541 3
TTTGT 3952200 2.1741292 34.73682 2
GAGGG 1655035 2.1660213 13.826559 11
GAGAG 2041715 2.135112 5.0488977 11
TGAGG 2015120 2.0076902 17.611328 10
GGGGG 1182810 1.9373161 5.96076 13
TTGAG 2551075 1.9349033 28.4499 9
AGGGG 1278940 1.6738085 7.9593205 12
TGAGA 1867590 1.4867821 5.4048166 10
TGAGC 1390115 1.4707942 6.142729 10
GAGTG 1319985 1.3151182 6.9930744 11
TGAGT 1707620 1.2951713 8.539096 10
TTGAT 1805505 1.0424973 8.705555 9
TTGAC 1145395 0.9225645 6.5997567 9
Produced by FastQC (version 0.10.1)