##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 140701_JD_C_9_ACTTGA_L003_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24153543 Filtered Sequences 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.068948766646784 34.0 33.0 34.0 31.0 34.0 2 33.25885336987621 34.0 34.0 34.0 31.0 34.0 3 33.27061487418223 34.0 34.0 34.0 31.0 34.0 4 36.570531660717435 37.0 37.0 37.0 35.0 37.0 5 36.500266234233216 37.0 37.0 37.0 35.0 37.0 6 36.45274591806262 37.0 37.0 37.0 35.0 37.0 7 36.46953815429894 37.0 37.0 37.0 35.0 37.0 8 36.43739347059767 37.0 37.0 37.0 35.0 37.0 9 38.2839924560964 39.0 39.0 39.0 37.0 39.0 10 38.247868480413 39.0 39.0 39.0 37.0 39.0 11 38.2234881648626 39.0 39.0 39.0 37.0 39.0 12 38.18218432798865 39.0 39.0 39.0 37.0 39.0 13 38.207168240286734 39.0 39.0 39.0 37.0 39.0 14 39.768338127454015 41.0 40.0 41.0 38.0 41.0 15 39.757576476461445 41.0 40.0 41.0 38.0 41.0 16 39.693272949645525 41.0 40.0 41.0 38.0 41.0 17 39.69954718444412 41.0 40.0 41.0 38.0 41.0 18 39.695841434111756 41.0 40.0 41.0 37.0 41.0 19 39.709866829889094 41.0 40.0 41.0 38.0 41.0 20 39.69533517298063 41.0 40.0 41.0 37.0 41.0 21 39.65368347823754 41.0 40.0 41.0 37.0 41.0 22 39.62151635476418 41.0 40.0 41.0 37.0 41.0 23 39.59517789998759 41.0 40.0 41.0 37.0 41.0 24 39.56762310191925 41.0 40.0 41.0 37.0 41.0 25 39.51829232671994 41.0 40.0 41.0 37.0 41.0 26 39.44627825408471 41.0 39.0 41.0 37.0 41.0 27 39.39731798353559 41.0 39.0 41.0 37.0 41.0 28 39.33424607727322 41.0 39.0 41.0 36.0 41.0 29 39.30316575087969 41.0 39.0 41.0 36.0 41.0 30 39.2042740479109 41.0 39.0 41.0 36.0 41.0 31 39.1769303989895 40.0 39.0 41.0 36.0 41.0 32 39.10917131287944 40.0 39.0 41.0 36.0 41.0 33 39.042735966313515 40.0 39.0 41.0 36.0 41.0 34 38.992190876510335 40.0 39.0 41.0 35.0 41.0 35 38.93937100656413 40.0 39.0 41.0 35.0 41.0 36 38.88491564156861 40.0 38.0 41.0 35.0 41.0 37 39.047706955455766 40.0 39.0 41.0 36.0 41.0 38 39.0695004455454 40.0 39.0 41.0 36.0 41.0 39 39.04617985030188 40.0 39.0 41.0 35.0 41.0 40 39.02121092545305 40.0 39.0 41.0 35.0 41.0 41 38.9875269230688 40.0 39.0 41.0 35.0 41.0 42 38.93674306912241 40.0 39.0 41.0 35.0 41.0 43 38.905115907840106 40.0 39.0 41.0 35.0 41.0 44 38.84997182400942 40.0 38.0 41.0 35.0 41.0 45 38.80561071309497 40.0 38.0 41.0 35.0 41.0 46 38.74138655351722 40.0 38.0 41.0 35.0 41.0 47 38.674286542558164 40.0 38.0 41.0 35.0 41.0 48 38.62683830691009 40.0 38.0 41.0 35.0 41.0 49 38.57969433304257 40.0 38.0 41.0 35.0 41.0 50 38.68199468707345 40.0 38.0 41.0 35.0 41.0 51 38.41739367181039 40.0 38.0 41.0 34.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 5.0 10 7.0 11 35.0 12 77.0 13 123.0 14 237.0 15 357.0 16 613.0 17 907.0 18 1477.0 19 2349.0 20 3835.0 21 6342.0 22 10256.0 23 15687.0 24 22819.0 25 31687.0 26 43341.0 27 58643.0 28 77139.0 29 99966.0 30 130662.0 31 168717.0 32 221694.0 33 296360.0 34 424254.0 35 653726.0 36 1002837.0 37 1705645.0 38 4154938.0 39 1.5002762E7 40 16041.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.471427108041578 27.510144509347096 28.68499167138314 24.333436711228185 2 21.39644341719146 27.189374556690048 32.74224650124158 18.671935524876908 3 20.435067124765144 26.18674507826806 34.02613854387225 19.35204925309455 4 21.260947099976182 26.00592799159941 33.37564182612878 19.35748308229563 5 26.221836301968715 26.40565214464375 28.463732949588056 18.90877860379948 6 21.447429058337324 26.019246948573965 32.77346101977668 19.759862973312032 7 26.16407456247723 26.699304528532313 27.84907787648379 19.28754303250666 8 21.231096406850124 26.69617869312175 32.412329735641684 19.660395164386443 9 20.59352291243756 26.85588419519937 32.86635694534395 19.68423594701911 10 21.10013425359584 26.653000762662437 32.629887880217 19.61697710352473 11 26.00867293050961 26.54030094052868 27.777038755763495 19.67398737319821 12 20.99281336382616 31.700119593720842 27.452041824929136 19.855025217523863 13 23.80294683889647 27.074367516185927 28.73688551613318 20.385800128784417 14 22.88160258890651 27.37528752540313 29.12174118900609 20.621368696684268 15 23.14437678977366 27.405507341096914 28.720179892448904 20.729935976680522 16 23.03423973865863 27.4002327526028 28.659654610505797 20.905872898232776 17 23.006753087942418 27.544360676195623 28.572586638738674 20.87629959712329 18 22.550529765184145 27.491188935139952 28.955614060226708 21.002667239449195 19 22.217172064908993 27.885366875688806 29.154193369235454 20.74326769016675 20 21.92490774844347 27.89920495588772 29.314919138628586 20.860968157040226 21 22.098112065795068 27.90802989027324 29.064791860970455 20.929066182961233 22 22.01648014951678 28.013736949481903 29.04997829924993 20.919804601751387 23 22.06338010121331 28.058537830247097 28.921446431275115 20.95663563726448 24 21.916619023552776 28.110025100665354 28.91685083219468 21.056505043587187 25 22.015039367102375 28.151108100372685 28.833066022653487 21.00078650987145 26 21.966436765732826 28.149250695859607 28.772040177637248 21.11227236077032 27 22.00252473185592 28.127972190687228 28.643091185961527 21.226411891495324 28 21.93879371943725 28.224478123419665 28.693243947443975 21.14348420969911 29 22.047474057892426 28.144979443017416 28.62578301002848 21.181763489061677 30 21.961109504949405 28.140004159229687 28.655809087134372 21.243077248686536 31 22.05655451212624 28.107842843787132 28.54247786302607 21.293124781060563 32 22.046393975716406 28.236684703077803 28.484589958116903 21.232331363088893 33 22.03821526834589 28.140498442561068 28.44934892283437 21.37193736625867 34 22.01062739540874 28.213593068291427 28.454782272040802 21.32099726425903 35 22.05107338536153 28.13905480382692 28.413943172734683 21.39592863807687 36 22.016287073678036 28.12900946254379 28.478887049275286 21.375816414502882 37 22.085722589828453 28.110532491266188 28.44217302343452 21.36157189547084 38 22.09736028944492 28.127086051337162 28.42635335941237 21.349200299805542 39 22.023557043225864 28.129194966745814 28.400711773151727 21.446536216876595 40 22.022625908389102 28.192194523656415 28.38115916419508 21.404020403759404 41 22.067078021563322 28.087191450188513 28.41065161737329 21.43507891087488 42 21.990017395074545 28.09747551568939 28.379479923126144 21.533027166109918 43 22.03693800655861 28.12366112188608 28.370619182530604 21.46878168902471 44 22.02444957486858 28.097120941061167 28.352347603776295 21.526081880293958 45 22.099772277715115 28.060723844944818 28.334141289333825 21.505362588006243 46 22.002300704114496 28.120555603813518 28.374486794509874 21.502656897562108 47 22.04837915962178 28.21375307833768 28.248464464177346 21.489403297863188 48 21.98489833083742 28.088716292706202 28.346886033732023 21.579499342724358 49 22.11877156076026 28.095637977418054 28.244038566101874 21.541551895719813 50 21.99959795216459 28.082892243520085 28.271287470206126 21.646222334109204 51 22.0452926201066 28.07951410832747 28.233547571634688 21.641645699931235 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.80486381926976 2 40.06837894206837 3 39.7871163778597 4 40.61843018227181 5 45.1306149057682 6 41.20729203164935 7 45.45161759498389 8 40.891491571236564 9 40.277758859456675 10 40.717111357120565 11 45.68266030370782 12 40.84783858135002 13 44.18874696768089 14 43.50297128559078 15 43.87431276645418 16 43.940112636891406 17 43.88305268506571 18 43.55319700463334 19 42.96043975507574 20 42.78587590548369 21 43.0271782487563 22 42.93628475126817 23 43.02001573847779 24 42.97312406713996 25 43.01582587697382 26 43.07870912650315 27 43.228936623351245 28 43.08227792913636 29 43.2292375469541 30 43.204186753635945 31 43.349679293186796 32 43.278725338805295 33 43.41015263460456 34 43.331624659667774 35 43.447002023438394 36 43.392103488180915 37 43.447294485299295 38 43.44656058925047 39 43.47009326010246 40 43.426646312148506 41 43.5021569324382 42 43.52304456118446 43 43.505719695583316 44 43.55053145516254 45 43.605134865721354 46 43.50495760167661 47 43.53778245748497 48 43.56439767356178 49 43.66032345648007 50 43.645820286273796 51 43.68693832003784 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27015.0 1 16188.0 2 5361.0 3 6936.5 4 8512.0 5 15686.5 6 22861.0 7 37511.5 8 52162.0 9 39058.5 10 25955.0 11 25954.5 12 25954.0 13 32590.5 14 39227.0 15 53993.0 16 68759.0 17 94519.5 18 120280.0 19 152490.0 20 184700.0 21 222967.5 22 261235.0 23 313558.0 24 365881.0 25 430946.0 26 573476.5 27 650942.0 28 719057.0 29 787172.0 30 852489.0 31 917806.0 32 996418.5 33 1075031.0 34 1147232.0 35 1219433.0 36 1314236.5 37 1409040.0 38 1474238.5 39 1539437.0 40 1600094.5 41 1660752.0 42 1688973.0 43 1717194.0 44 1725942.0 45 1734690.0 46 1706700.0 47 1678710.0 48 1625791.0 49 1572872.0 50 1499920.0 51 1426968.0 52 1320959.5 53 1214951.0 54 1112232.5 55 1009514.0 56 904671.0 57 799828.0 58 704144.0 59 608460.0 60 527716.0 61 446972.0 62 377409.5 63 307847.0 64 258176.0 65 208505.0 66 172944.5 67 137384.0 68 116635.0 69 95886.0 70 82216.5 71 68547.0 72 57640.0 73 46733.0 74 38720.5 75 25618.0 76 20528.0 77 18510.5 78 16493.0 79 15627.5 80 14762.0 81 12835.0 82 10908.0 83 9427.5 84 7947.0 85 6698.5 86 5450.0 87 5603.5 88 5757.0 89 3760.0 90 1763.0 91 1107.5 92 452.0 93 299.0 94 146.0 95 89.5 96 33.0 97 20.0 98 7.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25133786790617013 2 0.011729128103483618 3 0.011157783352943292 4 0.0 5 8.69437663865711E-5 6 0.0 7 0.0 8 0.0 9 8.280358703482963E-6 10 0.0 11 0.0 12 4.181581145258896E-4 13 0.0 14 1.0350448379353704E-4 15 0.0 16 0.0 17 0.0 18 4.38859011284597E-4 19 0.0011592502184876148 20 0.0012089323707085125 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.005800391271789816 27 0.0019997066268911358 28 0.004906112531813655 29 0.001805118197359286 30 1.324857392557274E-4 31 7.203912072030178E-4 32 0.0015070252840338992 33 4.4713936998808004E-4 34 0.0012668948816328934 35 4.2229829387763115E-4 36 0.002169453980312536 37 0.0011716707565428393 38 0.0022936593608647807 39 0.0014159413382955866 40 0.001436642235054294 41 1.1592502184876147E-4 42 0.00301819074741954 43 0.0035564140631459324 44 0.003701320340456884 45 0.0 46 0.0038793480525817683 47 3.519152448980259E-4 48 2.2356968499404002E-4 49 0.0 50 0.009058712421610363 51 0.004136039172389741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2.4153543E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 20.90260358941453 #Duplication Level Relative count 1 100.0 2 10.830526807443643 3 2.9904864918016667 4 1.3803190835566264 5 0.8370412252021928 6 0.5985889525307898 7 0.42036431574030825 8 0.3761154403992232 9 0.26057671034194546 10++ 5.219523587108828 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27739 0.11484443503795695 No Hit >>END_MODULE >>Kmer Content warn #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TTTTT 9264165 3.8796535 4.9007897 16 TGTGT 5334975 3.8551264 46.084595 4 AAAAA 5846790 3.119271 3.2369685 46 TGTTT 4787910 2.6338584 35.095196 6 GTTTG 3613750 2.6113455 45.171577 7 CTTTG 3310140 2.5401418 48.03999 1 GTGTT 3365035 2.4316206 45.22509 5 TTTGA 3975460 2.2954278 36.28045 8 TTGTG 3105835 2.2443192 45.19541 3 TTTGT 3952200 2.1741292 34.73682 2 GAGGG 1655035 2.1660213 13.826559 11 GAGAG 2041715 2.135112 5.0488977 11 TGAGG 2015120 2.0076902 17.611328 10 GGGGG 1182810 1.9373161 5.96076 13 TTGAG 2551075 1.9349033 28.4499 9 AGGGG 1278940 1.6738085 7.9593205 12 TGAGA 1867590 1.4867821 5.4048166 10 TGAGC 1390115 1.4707942 6.142729 10 GAGTG 1319985 1.3151182 6.9930744 11 TGAGT 1707620 1.2951713 8.539096 10 TTGAT 1805505 1.0424973 8.705555 9 TTGAC 1145395 0.9225645 6.5997567 9 >>END_MODULE