FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_8_TGACCA_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_8_TGACCA_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29498503
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 7252730 4.236372 36.925755 4
TTTTT 10129825 3.8330812 4.8508353 18
AAAAA 6518360 3.2402945 3.3462179 43
GTTTG 5300440 3.0960255 36.345177 7
TGTTT 6499010 3.0553892 29.802576 6
GTGTT 4974565 2.9056792 36.281757 5
TTTGA 5700640 2.830368 31.076042 8
CTTTG 4355000 2.751281 39.361874 1
GGGGG 2378915 2.6649628 5.1831026 13
TTGTG 4553825 2.659922 36.2672 3
TTTGT 4998690 2.3500414 29.43019 2
GAGGG 2358720 2.246029 10.223059 11
TTGAG 3575210 2.205434 22.7044 9
TGAGG 2634315 2.0189867 13.32449 10
ACTTT 3121980 1.6765008 16.723532 3
AGGGG 1756640 1.6727142 5.758342 12
AACTT 2842980 1.6123058 18.164433 2
TGAGC 1784925 1.4795854 5.3258643 10
TGAGT 2119695 1.307573 6.6734943 10
GAGTG 1684510 1.291039 5.228337 11
TAACT 2207250 1.2517717 18.008469 1
TTGAT 2323585 1.15366 6.883443 9
TTGAC 1563605 1.043215 6.6060276 9
GTAAC 1040090 0.7328546 5.6112876 1