##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_8_TGACCA_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29498503	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.142108160539536	34.0	31.0	34.0	31.0	34.0
2	32.30390745591395	34.0	31.0	34.0	31.0	34.0
3	32.38606287919085	34.0	31.0	34.0	31.0	34.0
4	35.77954545693387	37.0	37.0	37.0	35.0	37.0
5	35.79659472889183	37.0	37.0	37.0	35.0	37.0
6	35.80812677172126	37.0	37.0	37.0	35.0	37.0
7	35.833588436674226	37.0	37.0	37.0	35.0	37.0
8	35.83302776415468	37.0	37.0	37.0	35.0	37.0
9	37.56779054177766	39.0	38.0	39.0	35.0	39.0
10	37.5407950023769	39.0	38.0	39.0	35.0	39.0
11	37.53432725043708	39.0	38.0	39.0	35.0	39.0
12	37.49698779629597	39.0	38.0	39.0	35.0	39.0
13	37.48968596813201	39.0	38.0	39.0	35.0	39.0
14	38.96789677767716	41.0	39.0	41.0	36.0	41.0
15	38.91382115221237	41.0	39.0	41.0	36.0	41.0
16	38.885816680256625	41.0	39.0	41.0	36.0	41.0
17	38.856300233269465	41.0	39.0	41.0	36.0	41.0
18	38.82239752980007	41.0	39.0	41.0	36.0	41.0
19	38.832384612873405	41.0	39.0	41.0	36.0	41.0
20	38.79634756380688	41.0	39.0	41.0	35.0	41.0
21	38.75679721781136	41.0	39.0	41.0	35.0	41.0
22	38.711230532613804	41.0	39.0	41.0	35.0	41.0
23	38.68302513520771	41.0	39.0	41.0	35.0	41.0
24	38.65239171628472	41.0	39.0	41.0	35.0	41.0
25	38.607647886402916	41.0	39.0	41.0	35.0	41.0
26	38.569954075296636	41.0	39.0	41.0	35.0	41.0
27	38.51413409012654	40.0	39.0	41.0	34.0	41.0
28	38.47808398955025	40.0	39.0	41.0	34.0	41.0
29	38.41468941661209	40.0	39.0	41.0	34.0	41.0
30	38.3714233566361	40.0	38.0	41.0	34.0	41.0
31	38.30526769443181	40.0	38.0	41.0	34.0	41.0
32	38.25883110068331	40.0	38.0	41.0	34.0	41.0
33	38.19056261939801	40.0	38.0	41.0	34.0	41.0
34	38.14809185401713	40.0	38.0	41.0	34.0	41.0
35	38.10134975324002	40.0	38.0	41.0	33.0	41.0
36	38.05070335264132	40.0	38.0	41.0	33.0	41.0
37	38.00155902826662	40.0	38.0	41.0	33.0	41.0
38	37.9453741771235	40.0	38.0	41.0	33.0	41.0
39	37.885460153689834	40.0	38.0	41.0	33.0	41.0
40	37.81681412782201	40.0	38.0	41.0	33.0	41.0
41	37.747364705253005	40.0	38.0	41.0	33.0	41.0
42	37.67244012348694	40.0	38.0	41.0	33.0	41.0
43	37.62368029319996	40.0	38.0	41.0	32.0	41.0
44	37.55177620369413	40.0	38.0	41.0	32.0	41.0
45	37.48413673737952	40.0	37.0	41.0	32.0	41.0
46	37.42476460585135	40.0	37.0	41.0	32.0	41.0
47	37.34940040855633	40.0	37.0	41.0	31.0	41.0
48	37.57146791482944	40.0	37.0	41.0	32.0	41.0
49	37.60272200931688	40.0	37.0	41.0	32.0	41.0
50	37.58178220094762	40.0	37.0	41.0	32.0	41.0
51	36.07883610229305	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111261.0
3	3081.0
4	1795.0
5	1493.0
6	1816.0
7	3879.0
8	10326.0
9	16287.0
10	18373.0
11	21351.0
12	27296.0
13	34422.0
14	40934.0
15	44335.0
16	46608.0
17	47719.0
18	49052.0
19	51146.0
20	53628.0
21	57634.0
22	63475.0
23	70948.0
24	80069.0
25	93826.0
26	114549.0
27	128542.0
28	150353.0
29	178388.0
30	218741.0
31	273563.0
32	353713.0
33	471470.0
34	671820.0
35	998067.0
36	1504739.0
37	2435326.0
38	5275791.0
39	1.5765066E7
40	7621.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.85536679486773	26.593033550544394	30.072332674290063	23.479266980297812
2	21.8627410397576	28.755673640528016	30.9764206523326	18.405164667381786
3	22.367147421704146	27.64214180116226	30.577089989985218	19.413620787148375
4	21.448910613348986	25.01964276735319	32.03968711108786	21.491759508209967
5	26.553291247029126	25.01334592931527	29.095808732657748	19.337554090997862
6	21.133102904205817	24.595188691300866	34.43855483747773	19.833153567015586
7	25.269034685930375	25.049929603760695	30.44619393087365	19.234841779435282
8	23.123884017333076	25.293502867128332	32.03721411794878	19.54539899758982
9	22.63405360987408	25.568420872098635	32.24997954855459	19.547545969472697
10	22.985761740017367	26.821922288467658	30.649605190456914	19.54271078105806
11	26.1605936426616	25.18777843932761	28.818642077026386	19.83298584098441
12	21.566278594771244	29.48558346949891	28.98247889433551	19.965659041394336
13	24.075232032665234	25.691751680189594	29.685263451387023	20.54775283575815
14	25.135271174722472	26.156751816366903	27.976386915523232	20.731590093387393
15	23.782660079832667	27.99520088865472	27.31030515231401	20.911833879198603
16	24.366304260866023	26.643035022132587	27.757193546939195	21.23346717006219
17	24.031275763761375	26.6409233023994	28.007472865263537	21.32032806857568
18	23.858637800126214	26.586404825105646	28.12623300749512	21.42872436727302
19	23.509797205501908	26.912861252953814	28.372166836564734	21.205174704979548
20	23.150468663074655	26.98661031210925	28.45399649744743	21.40892452736867
21	23.165323770319183	27.09659370908417	28.321556112446935	21.416526408149714
22	22.87273066993249	27.311515231769945	28.542640172094764	21.2731139262028
23	22.861886500957816	27.41781425998242	28.376081575887426	21.34421766317234
24	22.69036516675164	27.489876001771613	28.330172877270144	21.489585954206607
25	22.71022189008482	27.637961806892207	28.22655627334575	21.425260029677215
26	22.71199713155714	27.570384784291203	28.190824427484035	21.52679365666762
27	22.682022954112536	27.624178222963845	28.08026554157051	21.61353328135311
28	22.663853327468427	27.689019383963796	28.053195933477003	21.593931355090767
29	22.751933309113028	27.63675424891195	27.940491996319356	21.670820445655664
30	22.773121887570777	27.598825851021424	27.903743067935675	21.724309193472124
31	22.839567091358802	27.58957928263018	27.814365213439874	21.756488412571148
32	22.882094029865534	27.637041805749433	27.727051977392552	21.75381218699248
33	22.873578230121506	27.559784998236054	27.700618003759132	21.866018767883304
34	22.857577118168944	27.58588022400088	27.709052795977218	21.84748986185296
35	22.951194791514524	27.529295688046503	27.636383816326187	21.883125704112786
36	22.880924145746732	27.53792276866215	27.65884282744981	21.92231025814131
37	22.86209245397539	27.500160844876437	27.671466595497773	21.966280105650405
38	22.850485611676728	27.553708772984724	27.744820673775667	21.850984941562878
39	22.840747424968562	27.439679231502012	27.716946841416405	22.00262650211302
40	22.84195529392771	27.467643794548074	27.737894640403365	21.952506271120846
41	22.867074682339037	27.46495244610228	27.720748161440632	21.94722471011805
42	22.76562080694618	27.458847624509435	27.721117393928086	22.054414174616298
43	22.841098781919158	27.514710869685675	27.633405985150056	22.010784363245108
44	22.891830916413838	27.430633671014583	27.668201521579704	22.009333890991876
45	22.86769051117	27.44734310662562	27.57581964785041	22.109146734353974
46	22.7657847926587	27.576247728004148	27.528685341178427	22.129282138158725
47	22.822882008972645	27.488689546705537	27.57257786275858	22.11585058156324
48	22.74764769552331	27.38465836634885	27.544330582600164	22.32336335552768
49	22.81921229213993	27.485807782744775	27.536839997404964	22.158139927710323
50	22.739588543216012	27.474888365494316	27.562366722347715	22.22315636894196
51	22.766821247950926	27.44139142426429	27.52346510120417	22.268322226580615
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.33463377516554
2	40.26790570713939
3	41.78076820885253
4	42.94067012155895
5	45.890845338026985
6	40.9662564712214
7	44.50387646536566
8	42.6692830149229
9	42.18159957934677
10	42.52847252107543
11	45.99357948364601
12	41.53193763616558
13	44.62298486842338
14	45.866861268109865
15	44.69449395903127
16	45.59977143092821
17	45.351603832337055
18	45.28736216739923
19	44.71497191048145
20	44.559393190443316
21	44.5818501784689
22	44.14584459613529
23	44.20610416413015
24	44.17995112095824
25	44.135481919762036
26	44.23879078822476
27	44.29555623546565
28	44.2577846825592
29	44.42275375476869
30	44.4974310810429
31	44.596055503929946
32	44.63590621685801
33	44.739596998004814
34	44.705066980021904
35	44.83432049562731
36	44.80323440388804
37	44.82837255962579
38	44.70147055323961
39	44.84337392708158
40	44.79446156504856
41	44.81429939245709
42	44.820034981562486
43	44.851883145164265
44	44.90116480740571
45	44.97683724552397
46	44.89506693081742
47	44.938732590535885
48	45.07101105105099
49	44.97735221985025
50	44.96274491215797
51	45.035143474531544
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	122909.0
1	65357.5
2	7806.0
3	8581.5
4	9357.0
5	22332.5
6	35308.0
7	43785.0
8	52262.0
9	40053.5
10	27845.0
11	28228.0
12	28611.0
13	35376.5
14	42142.0
15	57302.0
16	72462.0
17	98133.0
18	123804.0
19	156791.0
20	189778.0
21	231999.0
22	274220.0
23	330398.0
24	386576.0
25	456962.0
26	618647.0
27	709946.0
28	790640.0
29	871334.0
30	950297.0
31	1029260.0
32	1125521.5
33	1221783.0
34	1306220.0
35	1390657.0
36	1508804.5
37	1626952.0
38	1715412.0
39	1803872.0
40	1886617.5
41	1969363.0
42	2006973.0
43	2044583.0
44	2061312.0
45	2078041.0
46	2058383.5
47	2038726.0
48	1992407.0
49	1946088.0
50	1863200.5
51	1780313.0
52	1652797.0
53	1525281.0
54	1398958.5
55	1272636.0
56	1155806.5
57	1038977.0
58	921091.5
59	803206.0
60	710828.5
61	618451.0
62	536389.5
63	454328.0
64	393857.5
65	333387.0
66	280778.5
67	228170.0
68	213887.5
69	199605.0
70	176553.5
71	153502.0
72	131190.0
73	108878.0
74	92065.5
75	63208.5
76	51164.0
77	48257.5
78	45351.0
79	45154.5
80	44958.0
81	39595.5
82	34233.0
83	31427.0
84	28621.0
85	25942.5
86	23264.0
87	26576.5
88	29889.0
89	20377.0
90	10865.0
91	7179.5
92	3494.0
93	2695.0
94	1896.0
95	1450.5
96	1005.0
97	793.5
98	582.0
99	371.5
100	161.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8630810858435766
2	0.4975099922867272
3	0.48105152997085987
4	0.48879429576477157
5	0.4851432630327037
6	0.4400901293194438
7	0.41576347111580547
8	0.3848534279858202
9	0.3790938136759008
10	0.39103001260775844
11	0.40002029933519
12	0.41528548075812527
13	0.39763034754678905
14	0.3702018370220347
15	0.42566566852561977
16	0.3803582846221044
17	0.4184110630969985
18	0.41240736860443394
19	0.4138006596470336
20	0.41236668857399306
21	0.4390731285584221
22	0.4660236487254964
23	0.43212701336064413
24	0.4437716720743422
25	0.41961112399500405
26	0.40509513313268813
27	0.3916571630770551
28	0.38875871090814335
29	0.42867938078078066
30	0.41470918032687965
31	0.45165342797226016
32	0.4393443287613612
33	0.42997775175235164
34	0.41301417905851023
35	0.42860819072750916
36	0.43063880224701573
37	0.41484478042834916
38	0.4242113574373587
39	0.4154482008798887
40	0.42768950004005285
41	0.43191683320336627
42	0.45582991109752247
43	0.4006542298095601
44	0.44230041097339756
45	0.4371035370845768
46	0.41476342036746744
47	0.42753694992589963
48	0.4286827707833174
49	0.45662656169365606
50	0.44490054291907627
51	0.4316558030080374
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9498503E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.61576122997042
#Duplication Level	Relative count
1	100.0
2	10.813237395515877
3	2.999532113456164
4	1.3796330252026454
5	0.8763388510223953
6	0.6139430190063101
7	0.4590346362498261
8	0.3622959319161851
9	0.2782028098483795
10++	8.673604876136523
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	7252730	4.236372	36.925755	4
TTTTT	10129825	3.8330812	4.8508353	18
AAAAA	6518360	3.2402945	3.3462179	43
GTTTG	5300440	3.0960255	36.345177	7
TGTTT	6499010	3.0553892	29.802576	6
GTGTT	4974565	2.9056792	36.281757	5
TTTGA	5700640	2.830368	31.076042	8
CTTTG	4355000	2.751281	39.361874	1
GGGGG	2378915	2.6649628	5.1831026	13
TTGTG	4553825	2.659922	36.2672	3
TTTGT	4998690	2.3500414	29.43019	2
GAGGG	2358720	2.246029	10.223059	11
TTGAG	3575210	2.205434	22.7044	9
TGAGG	2634315	2.0189867	13.32449	10
ACTTT	3121980	1.6765008	16.723532	3
AGGGG	1756640	1.6727142	5.758342	12
AACTT	2842980	1.6123058	18.164433	2
TGAGC	1784925	1.4795854	5.3258643	10
TGAGT	2119695	1.307573	6.6734943	10
GAGTG	1684510	1.291039	5.228337	11
TAACT	2207250	1.2517717	18.008469	1
TTGAT	2323585	1.15366	6.883443	9
TTGAC	1563605	1.043215	6.6060276	9
GTAAC	1040090	0.7328546	5.6112876	1
>>END_MODULE