FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_8_TGACCA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_8_TGACCA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29498503
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9253465 3.6494255 4.489201 18
TGTGT 5723060 3.5063875 38.111713 4
AAAAA 6363375 3.0384686 3.157561 11
TGTTT 5207845 2.5599644 30.57201 6
GTTTG 3991705 2.4456263 37.32106 7
CTTTG 3677645 2.36799 39.14933 1
TTTGA 4440780 2.268008 31.354593 8
GTGTT 3660180 2.2425084 37.28262 5
TTTGT 4266425 2.0972006 30.181213 2
GAGGG 2071130 2.048141 10.151362 11
TTGTG 3316585 2.0319953 37.309395 3
TGAGG 2302085 1.8264935 13.586787 10
TTGAG 2821880 1.7963028 22.853191 9
AGGGG 1543775 1.5266395 5.7432837 12
TGAGC 1718015 1.4325249 5.286826 10
GAGTG 1566795 1.2431083 5.2718086 11
TGAGT 1944505 1.2377988 6.7303057 10
TTGAT 2080345 1.0624797 7.049799 9
TTGAC 1406050 0.9406331 6.598236 9
Produced by FastQC (version 0.10.1)