##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_8_TGACCA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	29498503	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.891873496088934	34.0	31.0	34.0	31.0	34.0
2	33.08184544144494	34.0	33.0	34.0	31.0	34.0
3	33.12655187959877	34.0	33.0	34.0	31.0	34.0
4	36.48051523156955	37.0	37.0	37.0	35.0	37.0
5	36.39174801514504	37.0	37.0	37.0	35.0	37.0
6	36.36936281139419	37.0	37.0	37.0	35.0	37.0
7	36.371149207130955	37.0	37.0	37.0	35.0	37.0
8	36.352618504064424	37.0	37.0	37.0	35.0	37.0
9	38.1422266411282	39.0	39.0	39.0	37.0	39.0
10	38.108126707311214	39.0	38.0	39.0	37.0	39.0
11	38.10013792903321	39.0	38.0	39.0	37.0	39.0
12	38.064050538429015	39.0	38.0	39.0	37.0	39.0
13	38.060647518282536	39.0	38.0	39.0	37.0	39.0
14	39.569998857230146	41.0	40.0	41.0	37.0	41.0
15	39.5567391335079	41.0	40.0	41.0	37.0	41.0
16	39.519195024913635	41.0	39.0	41.0	37.0	41.0
17	39.496948099366264	41.0	39.0	41.0	37.0	41.0
18	39.460897693689745	41.0	39.0	41.0	37.0	41.0
19	39.44895023994946	41.0	39.0	41.0	37.0	41.0
20	39.41193236144898	41.0	39.0	41.0	37.0	41.0
21	39.38544528852871	41.0	39.0	41.0	36.0	41.0
22	39.34148553233362	41.0	39.0	41.0	36.0	41.0
23	39.30194562754591	41.0	39.0	41.0	36.0	41.0
24	39.270389246532275	41.0	39.0	41.0	36.0	41.0
25	39.22612154250675	40.0	39.0	41.0	36.0	41.0
26	39.13690389644518	40.0	39.0	41.0	36.0	41.0
27	39.07491369985792	40.0	39.0	41.0	36.0	41.0
28	39.022452936001535	40.0	39.0	41.0	36.0	41.0
29	38.94491998458363	40.0	39.0	41.0	35.0	41.0
30	38.89147415379011	40.0	38.0	41.0	35.0	41.0
31	38.803040818715445	40.0	38.0	41.0	35.0	41.0
32	38.73061216021708	40.0	38.0	41.0	35.0	41.0
33	38.62609912780998	40.0	38.0	41.0	35.0	41.0
34	38.57269329904639	40.0	38.0	41.0	35.0	41.0
35	38.50680290454061	40.0	38.0	41.0	34.0	41.0
36	38.43911862239246	40.0	38.0	41.0	34.0	41.0
37	38.63338024305843	40.0	38.0	41.0	35.0	41.0
38	38.65651758667211	40.0	38.0	41.0	35.0	41.0
39	38.65458006462226	40.0	38.0	41.0	35.0	41.0
40	38.618131943848134	40.0	38.0	41.0	35.0	41.0
41	38.57648623728465	40.0	38.0	41.0	35.0	41.0
42	38.50108898746489	40.0	38.0	41.0	34.0	41.0
43	38.45347619843624	40.0	38.0	41.0	34.0	41.0
44	38.378523140648866	40.0	38.0	41.0	34.0	41.0
45	38.32421719841173	40.0	38.0	41.0	34.0	41.0
46	38.26183840583368	40.0	38.0	41.0	34.0	41.0
47	38.17906078149118	40.0	38.0	41.0	34.0	41.0
48	38.127066142983594	40.0	38.0	41.0	34.0	41.0
49	38.078436692194174	40.0	38.0	41.0	33.0	41.0
50	38.17077744589276	40.0	38.0	41.0	34.0	41.0
51	37.907424183525514	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	12.0
12	37.0
13	93.0
14	186.0
15	298.0
16	511.0
17	955.0
18	1699.0
19	3261.0
20	5617.0
21	9964.0
22	16426.0
23	25469.0
24	37107.0
25	52088.0
26	71451.0
27	95744.0
28	126496.0
29	165687.0
30	215877.0
31	278019.0
32	361193.0
33	483110.0
34	687397.0
35	1019388.0
36	1539701.0
37	2543061.0
38	5614364.0
39	1.6132533E7
40	10751.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.616528454826057	27.632917972053594	27.724159508603087	24.026394064517262
2	22.475934391877058	27.191238913222413	30.844294722850695	19.488531972049834
3	21.09210084321906	26.010024983301694	32.53285768433741	20.365016489141837
4	21.89314827264285	25.82993448853998	32.02487936421723	20.25203787459994
5	25.7764368585077	26.251850814259964	28.19505450835929	19.776657818873044
6	22.05525141394463	25.794546252058957	31.467718887294044	20.682483446702363
7	25.78006416122201	26.41587608700008	27.65452877388388	20.14953097789403
8	22.04480681612894	26.413360705117817	30.986399547122783	20.555432931630463
9	21.295294205268654	26.68022848481498	31.47371580178153	20.550761508134837
10	21.83340964794044	26.395153001492993	31.256572579293262	20.51486477127331
11	25.597763384806342	26.233243090335804	27.58576596242867	20.583227562429187
12	21.707760588074965	30.35768187162099	27.216576695319183	20.717980844984858
13	23.849576366637994	26.762381806290303	28.16564623635308	21.22239559071862
14	23.258433491123967	26.970262188376438	28.408219558971577	21.363084761528018
15	23.419795235032776	27.01696760679686	28.091500100869528	21.471737057300842
16	23.364012521450668	26.99793301347299	28.013811821408268	21.624242643668072
17	23.25489849500478	27.135923085442414	27.924515752479472	21.684662667073333
18	23.041744543977792	27.057311937946842	28.15824913701871	21.74269438105666
19	22.784663704725883	27.34485848603086	28.379785032150522	21.490692777092736
20	22.520705542243956	27.378396117253818	28.46632929135421	21.63456904914802
21	22.66067535698337	27.391844257317054	28.233602227204546	21.713878158495024
22	22.541971028156922	27.475285779756348	28.332332661084532	21.6504105310022
23	22.660827907097524	27.519860923111928	28.142146738768403	21.677164431022145
24	22.446857449313505	27.54170806709089	28.1718352363121	21.839599247283505
25	22.51268818624457	27.69505625421059	28.064444490623814	21.727811068921024
26	22.51283490275843	27.58585443650457	28.01796460920727	21.88334605152973
27	22.53311305538091	27.598777080380092	27.916986102921086	21.95112376131791
28	22.52541028715847	27.673223635952027	27.94627832764027	21.855087749249233
29	22.63815557013181	27.65374229329536	27.842833244792114	21.865268891780712
30	22.58268834862569	27.56283598527017	27.895452864167382	21.95902280193676
31	22.70964075803433	27.555154060899145	27.727764971443737	22.007440209622793
32	22.720931435640995	27.62873119414553	27.696820874678984	21.95351649553449
33	22.654162525918693	27.565660553190934	27.667075896583874	22.113101024306495
34	22.63085011466514	27.593203627994274	27.741109438672197	22.034836818668392
35	22.769377224479793	27.55368712107459	27.618110479805363	22.058825174640255
36	22.735120661045627	27.57754343052159	27.61007824662258	22.077257661810197
37	22.73845791271496	27.510158674863895	27.62733777664265	22.124045635778497
38	22.697626384633825	27.56202238466135	27.685276096892103	22.055075133812725
39	22.638997501020732	27.483093684534683	27.683538155436267	22.19437065900832
40	22.679869551346385	27.53573291498894	27.69420875357641	22.090188780088262
41	22.687613879042406	27.4571770052373	27.746368922196606	22.10884019352369
42	22.606497102802035	27.511329219361713	27.672296715270488	22.20987696256576
43	22.674153336682885	27.558812959572514	27.61949745669886	22.14753624704574
44	22.736075793405515	27.439463622950626	27.64039585330822	22.18406473033564
45	22.773027493259228	27.389619941944783	27.572304011594905	22.265048553201087
46	22.65838710527107	27.54646227301772	27.59192898704046	22.20322163467075
47	22.656149703596824	27.600448063415286	27.537007555942754	22.20639467704514
48	22.624246389723574	27.411099471725738	27.576806185724067	22.387847952826622
49	22.763385653841482	27.458857827463312	27.53770928646786	22.240047232227344
50	22.62454131994427	27.435131199708678	27.54290955035922	22.397417929987835
51	22.658506339087403	27.461054022122966	27.518433232292	22.362006406497635
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.64292251934332
2	41.96446636392689
3	41.4571173323609
4	42.14518614724279
5	45.55309467738075
6	42.737734860646995
7	45.92959513911604
8	42.6002397477594
9	41.84605571340349
10	42.34827441921375
11	46.18099094723553
12	42.42574143305983
13	45.07197195735662
14	44.62151825265199
15	44.891532292333615
16	44.98825516511874
17	44.939561162078114
18	44.78443892503445
19	44.27535648181862
20	44.15527459139197
21	44.3745535154784
22	44.192381559159124
23	44.33799233811966
24	44.28645669659701
25	44.240499255165595
26	44.39618095428816
27	44.48423681669881
28	44.3804980364077
29	44.50342446191253
30	44.54171115056245
31	44.71708096765712
32	44.674447931175486
33	44.76726355022519
34	44.66568693333353
35	44.82820239912005
36	44.81237832285582
37	44.86250354849346
38	44.75270151844655
39	44.83336816002905
40	44.77005833143465
41	44.79645407256609
42	44.81637406536779
43	44.82168958372863
44	44.92014052374115
45	45.03807604646031
46	44.86160873994182
47	44.862544380641964
48	45.0120943425502
49	45.00343288606883
50	45.02195924993211
51	45.02051274558504
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24208.0
1	14534.0
2	4860.0
3	6418.5
4	7977.0
5	14547.0
6	21117.0
7	32045.5
8	42974.0
9	33099.5
10	23225.0
11	25238.0
12	27251.0
13	35009.5
14	42768.0
15	59275.0
16	75782.0
17	103854.5
18	131927.0
19	167449.5
20	202972.0
21	246178.0
22	289384.0
23	346390.0
24	403396.0
25	477617.5
26	643822.0
27	735805.0
28	810489.0
29	885173.0
30	960189.0
31	1035205.0
32	1125980.5
33	1216756.0
34	1297446.0
35	1378136.0
36	1492563.0
37	1606990.0
38	1688650.5
39	1770311.0
40	1851388.5
41	1932466.0
42	1968172.0
43	2003878.0
44	2024988.5
45	2046099.0
46	2025688.0
47	2005277.0
48	1971634.0
49	1937991.0
50	1857609.5
51	1777228.0
52	1667165.5
53	1557103.0
54	1429600.0
55	1302097.0
56	1184987.5
57	1067878.0
58	948153.0
59	828428.0
60	735624.0
61	642820.0
62	557367.0
63	471914.0
64	410927.5
65	349941.0
66	293817.5
67	237694.0
68	225971.0
69	214248.0
70	189351.0
71	164454.0
72	140804.5
73	117155.0
74	99063.5
75	66918.5
76	52865.0
77	50159.5
78	47454.0
79	47912.5
80	48371.0
81	42087.5
82	35804.0
83	32657.5
84	29511.0
85	26998.0
86	24485.0
87	28529.5
88	32574.0
89	20885.0
90	9196.0
91	5425.5
92	1655.0
93	1156.0
94	657.0
95	411.5
96	166.0
97	105.5
98	45.0
99	33.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27452240542511597
2	0.022807937067179308
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	7.559705656927743E-4
13	0.0
14	4.4748033484953454E-4
15	0.0
16	1.3560010146955593E-5
17	1.3560010146955593E-5
18	6.135904591497406E-4
19	0.005515534127274188
20	0.0
21	0.0
22	0.0
23	0.0
24	4.4070032977605676E-5
25	0.0
26	0.004732443541287502
27	0.004152753107505151
28	5.763004312456127E-5
29	0.0
30	0.0
31	0.0032103324022917366
32	0.0021729916260496336
33	2.7120020293911186E-5
34	0.0
35	0.004874823647830536
36	0.002956082212036319
37	2.3391017503498397E-4
38	0.0
39	2.0001014966759503E-4
40	0.0
41	3.356102511371509E-4
42	3.3900025367388982E-6
43	1.0170007610216695E-4
44	0.0
45	1.2204009132260035E-4
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	4.7460035514344574E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.9498503E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.770231551046695
#Duplication Level	Relative count
1	100.0
2	11.230062385695259
3	3.1740873144838044
4	1.5134425560929605
5	0.8946835152650595
6	0.6487092724642668
7	0.461361014994233
8	0.38170614362091926
9	0.3084236619574707
10++	8.835318332727956
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	9253465	3.6494255	4.489201	18
TGTGT	5723060	3.5063875	38.111713	4
AAAAA	6363375	3.0384686	3.157561	11
TGTTT	5207845	2.5599644	30.57201	6
GTTTG	3991705	2.4456263	37.32106	7
CTTTG	3677645	2.36799	39.14933	1
TTTGA	4440780	2.268008	31.354593	8
GTGTT	3660180	2.2425084	37.28262	5
TTTGT	4266425	2.0972006	30.181213	2
GAGGG	2071130	2.048141	10.151362	11
TTGTG	3316585	2.0319953	37.309395	3
TGAGG	2302085	1.8264935	13.586787	10
TTGAG	2821880	1.7963028	22.853191	9
AGGGG	1543775	1.5266395	5.7432837	12
TGAGC	1718015	1.4325249	5.286826	10
GAGTG	1566795	1.2431083	5.2718086	11
TGAGT	1944505	1.2377988	6.7303057	10
TTGAT	2080345	1.0624797	7.049799	9
TTGAC	1406050	0.9406331	6.598236	9
>>END_MODULE