FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_6_GTCCGC_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_6_GTCCGC_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 41850372
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 12204140 4.7635336 47.283066 4
TTTTT 17122620 4.3137336 5.650812 18
GTTTG 9136875 3.5663152 46.75831 7
AAAAA 9364195 3.4847467 3.7107182 44
GTGTT 8737570 3.410458 46.718735 5
TGTTT 10847550 3.401608 38.082268 6
TTTGA 9707210 3.29102 40.798107 8
TTGTG 7968405 3.1102366 46.723324 3
CTTTG 7151395 3.0804265 51.621304 1
GGGGG 3527505 2.6552043 5.9999075 13
TTTGT 8133970 2.5506752 37.74722 2
TTGAG 5955075 2.5130014 29.470125 9
GAGGG 3659125 2.3923333 13.185012 11
TGAGG 4140815 2.1750076 17.221024 10
ACTTT 4757875 1.7801113 20.36835 3
AACTT 4298085 1.7385719 22.68538 2
AGGGG 2627935 1.718142 7.271216 12
TGAGC 2639440 1.5299767 6.750938 10
TGAGA 3269965 1.4918739 5.4953537 10
TAACT 3393460 1.372652 22.55061 1
TGAGT 3184255 1.343734 8.342552 10
GAGTG 2521750 1.3245763 6.4778924 11
TTGAT 3677605 1.2468125 9.076061 9
TTGAC 2427850 1.1306429 8.725024 9
GTAAC 1557710 0.7842847 6.8931875 1