##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_6_GTCCGC_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41850372	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.11357722220486	34.0	31.0	34.0	31.0	34.0
2	32.28060828228719	34.0	31.0	34.0	31.0	34.0
3	32.36575478946758	34.0	31.0	34.0	31.0	34.0
4	35.75136452789476	37.0	37.0	37.0	35.0	37.0
5	35.77405495941589	37.0	37.0	37.0	35.0	37.0
6	35.77016307047402	37.0	36.0	37.0	35.0	37.0
7	35.81135204724106	37.0	37.0	37.0	35.0	37.0
8	35.80715148720781	37.0	37.0	37.0	35.0	37.0
9	37.536962706090165	39.0	38.0	39.0	35.0	39.0
10	37.51253967348247	39.0	38.0	39.0	35.0	39.0
11	37.50440046267689	39.0	38.0	39.0	35.0	39.0
12	37.460512250643795	39.0	38.0	39.0	35.0	39.0
13	37.4589419659161	39.0	38.0	39.0	35.0	39.0
14	38.917997885419034	41.0	39.0	41.0	36.0	41.0
15	38.85991371832967	41.0	39.0	41.0	36.0	41.0
16	38.82948672475361	41.0	39.0	41.0	36.0	41.0
17	38.7977837329618	41.0	39.0	41.0	36.0	41.0
18	38.761516313403376	41.0	39.0	41.0	35.0	41.0
19	38.77008584296455	41.0	39.0	41.0	35.0	41.0
20	38.73272297316736	41.0	39.0	41.0	35.0	41.0
21	38.69583627595951	41.0	39.0	41.0	35.0	41.0
22	38.64861148665536	41.0	39.0	41.0	35.0	41.0
23	38.619466249905734	41.0	39.0	41.0	35.0	41.0
24	38.58403547762968	41.0	39.0	41.0	35.0	41.0
25	38.53814360359807	41.0	39.0	41.0	34.0	41.0
26	38.498781802943114	40.0	39.0	41.0	34.0	41.0
27	38.44206421868843	40.0	39.0	41.0	34.0	41.0
28	38.40402183282863	40.0	38.0	41.0	34.0	41.0
29	38.33918833505231	40.0	38.0	41.0	34.0	41.0
30	38.2951602915262	40.0	38.0	41.0	34.0	41.0
31	38.22648381715699	40.0	38.0	41.0	34.0	41.0
32	38.1785287117639	40.0	38.0	41.0	34.0	41.0
33	38.109285432397115	40.0	38.0	41.0	33.0	41.0
34	38.06523894220104	40.0	38.0	41.0	33.0	41.0
35	38.01791835924421	40.0	38.0	41.0	33.0	41.0
36	37.96272023579623	40.0	38.0	41.0	33.0	41.0
37	37.911877151295094	40.0	38.0	41.0	33.0	41.0
38	37.85297528538098	40.0	38.0	41.0	33.0	41.0
39	37.79187604353911	40.0	38.0	41.0	33.0	41.0
40	37.7203170858314	40.0	38.0	41.0	33.0	41.0
41	37.649304789931136	40.0	38.0	41.0	32.0	41.0
42	37.57138696401552	40.0	38.0	41.0	32.0	41.0
43	37.521264422691395	40.0	37.0	41.0	32.0	41.0
44	37.4466003551892	40.0	37.0	41.0	32.0	41.0
45	37.37818884859614	40.0	37.0	41.0	31.0	41.0
46	37.31458817140263	40.0	37.0	41.0	31.0	41.0
47	37.23686477625575	40.0	37.0	41.0	31.0	41.0
48	37.46541043888451	40.0	37.0	41.0	32.0	41.0
49	37.49790118950436	40.0	37.0	41.0	32.0	41.0
50	37.47657891786481	40.0	37.0	41.0	32.0	41.0
51	35.96268322298306	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	155071.0
3	4362.0
4	2457.0
5	2237.0
6	2548.0
7	5679.0
8	13559.0
9	21198.0
10	24644.0
11	29951.0
12	39962.0
13	51126.0
14	60556.0
15	66472.0
16	68948.0
17	70557.0
18	72194.0
19	74852.0
20	78472.0
21	84744.0
22	93107.0
23	104906.0
24	118944.0
25	138367.0
26	168486.0
27	189663.0
28	220421.0
29	264326.0
30	324472.0
31	404677.0
32	523076.0
33	699505.0
34	995468.0
35	1490343.0
36	2242718.0
37	3568776.0
38	7609979.0
39	2.1753798E7
40	9751.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.21784511279783	25.12536666952176	31.48158466690089	24.17520355077952
2	21.14044917703193	27.895732276473318	33.06475044514484	17.89906810134991
3	22.63275987505111	27.04376757167276	31.61635729030536	18.707115262970767
4	21.065439090022302	23.986101452605844	33.67212287144159	21.276336585930274
5	28.40985744075029	23.794269409205317	29.084842274527844	18.711030875516542
6	20.44740571797957	23.42803462868065	37.0913677427471	19.033191910592677
7	26.1591900643388	23.895446091302748	31.527859352926725	18.41750449143173
8	23.188408751821328	24.15543663500949	33.963861622050196	18.692292991118983
9	23.086224624032877	24.69394142676475	33.49238400200314	18.727449947199233
10	23.030098270300233	26.726616862884644	31.474059300873687	18.76922556594144
11	27.777384625998792	24.272238474450404	28.715030028852915	19.235346870697885
12	21.501919724502873	30.15234761675415	28.98079334984956	19.36493930889342
13	24.909806050985118	24.88909447472526	29.966081375528848	20.23501809876078
14	26.13283685752068	25.502650943610934	27.87531616194869	20.4891960369197
15	24.484134604459946	27.813419092924217	27.01074145642423	20.691704846191605
16	25.128255015382162	26.15023461003973	27.629111192065846	21.09239918251226
17	24.641371171285346	26.217737897432464	27.983474974002547	21.157415957279646
18	24.347428092248176	26.191204973698373	28.195519009352388	21.265847924701063
19	23.907215972457678	26.60073567876966	28.52619669200791	20.96585165676475
20	23.391822476275877	26.707966913482238	28.67280039646087	21.22741021378101
21	23.47015863812729	26.85834400456159	28.474195146207027	21.197302211104095
22	23.020129871875675	27.132521624508428	28.80110675695554	21.046241746660353
23	22.99541589401825	27.28215921969413	28.585115851909134	21.13730903437849
24	22.778853537352514	27.38898623577064	28.551272164761805	21.280888062115043
25	22.80706511026737	27.601406806731983	28.354004403943016	21.23752367905763
26	22.791975679748813	27.49833148529236	28.336635976871577	21.373056858087246
27	22.786191868452647	27.54528798195775	28.221159880958314	21.44736026863129
28	22.740671342276066	27.63298539426345	28.190932153260583	21.4354111101999
29	22.879018456609355	27.53927872465204	28.02707254122239	21.554630277516225
30	22.868548820238686	27.533709334102248	28.006198316868964	21.591543528790105
31	22.92832441036064	27.495361383874172	27.89198088361647	21.684333322148717
32	22.979320073910976	27.572386844813536	27.792002647110404	21.65629043416508
33	22.96609770505748	27.473697769578152	27.77397519793292	21.786229327431446
34	22.967322092676458	27.51894336580989	27.752379096536522	21.76135544497713
35	23.076646757193327	27.437236783653628	27.680757158439604	21.805359300713434
36	22.99418813503008	27.455788787692065	27.722908818764445	21.827114258513408
37	22.96588997354326	27.394485363739925	27.748611861509016	21.8910128012078
38	22.923650139241545	27.466305369689543	27.831066389159364	21.778978101909548
39	22.928926325097585	27.339794167178326	27.78326341455725	21.948016093166835
40	22.93282219534183	27.40156350444026	27.82533756391524	21.84027673630266
41	22.937280372864514	27.39206899341119	27.801143374317	21.869507259407296
42	22.838635127956515	27.37212389350794	27.803638876629066	21.98560210190648
43	22.91783953652949	27.437166400786843	27.69280445238752	21.952189610296145
44	22.94895888338562	27.31434744522268	27.7542636819611	21.982429989430596
45	22.94436286278109	27.355412153570967	27.655928700207323	22.044296283440616
46	22.82616965236781	27.484517467766633	27.583702411770446	22.10561046809511
47	22.902398082041152	27.394917021289665	27.64295715652504	22.05972774014414
48	22.824599060084296	27.27528588002817	27.582398596725294	22.317716463162242
49	22.88517982490849	27.38991806121403	27.60109626792649	22.12380584595099
50	22.782097706972106	27.39851789011799	27.6270284500135	22.192355952896403
51	22.82663673696502	27.348316869421808	27.587451486896885	22.23759490671629
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	43.393048663577346
2	39.03951727838184
3	41.33987513802188
4	42.341775675952576
5	47.120888316266836
6	39.48059762857225
7	44.57669455577053
8	41.88070174294031
9	41.813674571232106
10	41.79932383624167
11	47.012731496696674
12	40.86685903339629
13	45.144824149745894
14	46.62203289444038
15	45.17583945065155
16	46.220654197894426
17	45.798787128564996
18	45.613276016949236
19	44.87306762922243
20	44.61923269005689
21	44.66746084923138
22	44.066371618536024
23	44.13272492839674
24	44.059741599467564
25	44.044588789325005
26	44.165032537836055
27	44.23355213708394
28	44.17608245247597
29	44.43364873412558
30	44.46009234902879
31	44.61265773250936
32	44.635610508076056
33	44.75232703248893
34	44.72867753765359
35	44.882006057906764
36	44.82130239354349
37	44.85690277475106
38	44.702628241151096
39	44.87694241826442
40	44.77309893164449
41	44.80678763227181
42	44.824237229862995
43	44.87002914682564
44	44.93138887281622
45	44.98865914622171
46	44.931780120462925
47	44.96212582218529
48	45.14231552324654
49	45.00898567085949
50	44.97445365986851
51	45.06423164368131
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177377.0
1	94604.5
2	11832.0
3	13277.0
4	14722.0
5	44714.5
6	74707.0
7	91989.5
8	109272.0
9	79766.5
10	50261.0
11	46688.5
12	43116.0
13	51526.5
14	59937.0
15	79074.5
16	98212.0
17	134975.5
18	171739.0
19	217351.0
20	262963.0
21	322037.5
22	381112.0
23	459290.0
24	537468.0
25	634899.0
26	854228.5
27	976127.0
28	1088480.0
29	1200833.0
30	1315756.5
31	1430680.0
32	1564662.0
33	1698644.0
34	1826952.5
35	1955261.0
36	2129974.5
37	2304688.0
38	2437405.5
39	2570123.0
40	2696392.5
41	2822662.0
42	2882649.0
43	2942636.0
44	2966115.5
45	2989595.0
46	2960883.5
47	2932172.0
48	2860835.5
49	2789499.0
50	2659938.5
51	2530378.0
52	2345522.0
53	2160666.0
54	1975438.0
55	1790210.0
56	1621933.0
57	1453656.0
58	1285841.5
59	1118027.0
60	990039.5
61	862052.0
62	748500.5
63	634949.0
64	551914.5
65	468880.0
66	394431.0
67	319982.0
68	303252.0
69	286522.0
70	254236.5
71	221951.0
72	189608.5
73	157266.0
74	132920.0
75	91065.0
76	73556.0
77	69582.0
78	65608.0
79	65251.0
80	64894.0
81	57468.0
82	50042.0
83	45988.5
84	41935.0
85	37739.0
86	33543.0
87	38352.0
88	43161.0
89	29557.5
90	15954.0
91	10502.0
92	5050.0
93	3961.0
94	2872.0
95	2204.5
96	1537.0
97	1201.5
98	866.0
99	569.5
100	273.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8537223994090184
2	0.4910350617671929
3	0.4742156174860286
4	0.48196226308334844
5	0.4784927598731978
6	0.4330021248078751
7	0.408627192131052
8	0.3782427549270052
9	0.37252237566729396
10	0.3841471229933153
11	0.3929809751750833
12	0.4084886031598477
13	0.39077310949589644
14	0.36367896562544294
15	0.41842160925116745
16	0.3734518775603715
17	0.4115925182218213
18	0.40543964579335157
19	0.40674429369468923
20	0.40522459394148275
21	0.4317572135320565
22	0.4589779990486106
23	0.4245792606096787
24	0.4364501228328389
25	0.4127107878515393
26	0.398065756739271
27	0.38496909896045844
28	0.3819201415939624
29	0.42153030324318264
30	0.40780999509395044
31	0.44445483065240143
32	0.4319770443139669
33	0.4227202568235236
34	0.40608719081397887
35	0.42112170472463184
36	0.42376206357257706
37	0.4077215848881821
38	0.4171814769053905
39	0.4081660253820444
40	0.4206462011854996
41	0.42473935476606994
42	0.44832098505599904
43	0.3939558769035553
44	0.4348467918039056
45	0.42959713715328507
46	0.407783710978722
47	0.4207083272760395
48	0.4215541978933903
49	0.4490497718873323
50	0.4376329080181175
51	0.42439288233805905
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.1850372E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.035825479537998
#Duplication Level	Relative count
1	100.0
2	11.934889954394343
3	3.445471637965246
4	1.6758940595540925
5	0.9940862278721481
6	0.6876630516105108
7	0.522415733626528
8	0.41897351488852314
9	0.36627653553142625
10++	10.070327697142002
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	12204140	4.7635336	47.283066	4
TTTTT	17122620	4.3137336	5.650812	18
GTTTG	9136875	3.5663152	46.75831	7
AAAAA	9364195	3.4847467	3.7107182	44
GTGTT	8737570	3.410458	46.718735	5
TGTTT	10847550	3.401608	38.082268	6
TTTGA	9707210	3.29102	40.798107	8
TTGTG	7968405	3.1102366	46.723324	3
CTTTG	7151395	3.0804265	51.621304	1
GGGGG	3527505	2.6552043	5.9999075	13
TTTGT	8133970	2.5506752	37.74722	2
TTGAG	5955075	2.5130014	29.470125	9
GAGGG	3659125	2.3923333	13.185012	11
TGAGG	4140815	2.1750076	17.221024	10
ACTTT	4757875	1.7801113	20.36835	3
AACTT	4298085	1.7385719	22.68538	2
AGGGG	2627935	1.718142	7.271216	12
TGAGC	2639440	1.5299767	6.750938	10
TGAGA	3269965	1.4918739	5.4953537	10
TAACT	3393460	1.372652	22.55061	1
TGAGT	3184255	1.343734	8.342552	10
GAGTG	2521750	1.3245763	6.4778924	11
TTGAT	3677605	1.2468125	9.076061	9
TTGAC	2427850	1.1306429	8.725024	9
GTAAC	1557710	0.7842847	6.8931875	1
>>END_MODULE