FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_6_GTCCGC_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_6_GTCCGC_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 41850372
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 47046 0.11241477136690685 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 14937555 4.024978 5.0911965 18
TGTGT 9253835 3.871551 49.941017 4
AAAAA 9448660 3.3222208 3.5052342 44
TGTTT 8372725 2.8111916 40.084923 6
GTTTG 6634605 2.7757368 49.224648 7
CTTTG 5955100 2.6462073 52.18934 1
GTGTT 6219880 2.602227 49.22366 5
TTTGA 7288115 2.5807958 41.906406 8
TTGTG 5535170 2.3157635 49.203903 3
TTTGT 6833435 2.2943656 39.689518 2
GAGGG 3126145 2.141738 13.117005 11
GGGGG 2572910 2.0826035 5.2831845 13
TTGAG 4534990 2.0010338 29.940855 9
TGAGG 3492775 1.9203848 17.635654 10
AGGGG 2268220 1.5539691 7.1775756 12
TGAGC 2493530 1.4561424 6.6169405 10
TGAGA 3118055 1.4510309 5.5482755 10
TGAGT 2862945 1.2632551 8.5103445 10
GAGTG 2295780 1.2622573 6.5510173 11
TTGAT 3188375 1.1290362 9.583297 9
TTGAC 2121275 0.994138 8.783105 9
Produced by FastQC (version 0.10.1)