##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_6_GTCCGC_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	41850372	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85847150892709	34.0	31.0	34.0	31.0	34.0
2	33.05186214354319	34.0	33.0	34.0	31.0	34.0
3	33.09386951207984	34.0	33.0	34.0	31.0	34.0
4	36.45895047241157	37.0	37.0	37.0	35.0	37.0
5	36.366636549849545	37.0	37.0	37.0	35.0	37.0
6	36.33066081228621	37.0	37.0	37.0	35.0	37.0
7	36.34197392558422	37.0	37.0	37.0	35.0	37.0
8	36.31785595597573	37.0	37.0	37.0	35.0	37.0
9	38.10791330600359	39.0	38.0	39.0	37.0	39.0
10	38.07709632784148	39.0	38.0	39.0	37.0	39.0
11	38.063359126174554	39.0	38.0	39.0	37.0	39.0
12	38.02272512655323	39.0	38.0	39.0	35.0	39.0
13	38.02267836472278	39.0	38.0	39.0	35.0	39.0
14	39.51306296632202	41.0	39.0	41.0	37.0	41.0
15	39.49966373058763	41.0	39.0	41.0	37.0	41.0
16	39.4589127905482	41.0	39.0	41.0	37.0	41.0
17	39.43643172395218	41.0	39.0	41.0	37.0	41.0
18	39.39646921179099	41.0	39.0	41.0	36.0	41.0
19	39.38387376819494	41.0	39.0	41.0	36.0	41.0
20	39.34441863503627	41.0	39.0	41.0	36.0	41.0
21	39.31927979039231	41.0	39.0	41.0	36.0	41.0
22	39.27403390822906	40.0	39.0	41.0	36.0	41.0
23	39.23601173724334	40.0	39.0	41.0	36.0	41.0
24	39.20197323455094	40.0	39.0	41.0	36.0	41.0
25	39.15569113698679	40.0	39.0	41.0	36.0	41.0
26	39.06005387478993	40.0	39.0	41.0	36.0	41.0
27	38.99618206500052	40.0	39.0	41.0	35.0	41.0
28	38.942778214731284	40.0	39.0	41.0	35.0	41.0
29	38.86537675220665	40.0	38.0	41.0	35.0	41.0
30	38.8110118830007	40.0	38.0	41.0	35.0	41.0
31	38.72042714459025	40.0	38.0	41.0	35.0	41.0
32	38.64501992957195	40.0	38.0	41.0	35.0	41.0
33	38.53769995162767	40.0	38.0	41.0	34.0	41.0
34	38.483716918932046	40.0	38.0	41.0	34.0	41.0
35	38.41607995742547	40.0	38.0	41.0	34.0	41.0
36	38.34693801527021	40.0	38.0	41.0	34.0	41.0
37	38.544376069106384	40.0	38.0	41.0	35.0	41.0
38	38.56679634771228	40.0	38.0	41.0	35.0	41.0
39	38.56499512119032	40.0	38.0	41.0	35.0	41.0
40	38.52766450917091	40.0	38.0	41.0	35.0	41.0
41	38.484663362132125	40.0	38.0	41.0	34.0	41.0
42	38.408425210652844	40.0	38.0	41.0	34.0	41.0
43	38.3572067650916	40.0	38.0	41.0	34.0	41.0
44	38.2804825486378	40.0	38.0	41.0	34.0	41.0
45	38.22501381827622	40.0	38.0	41.0	34.0	41.0
46	38.16014423957809	40.0	38.0	41.0	34.0	41.0
47	38.074171718234666	40.0	38.0	41.0	33.0	41.0
48	38.02122607177781	40.0	37.0	41.0	33.0	41.0
49	37.9712091448076	40.0	37.0	41.0	33.0	41.0
50	38.062340903445254	40.0	37.0	41.0	33.0	41.0
51	37.79674725471974	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	11.0
11	37.0
12	90.0
13	160.0
14	328.0
15	555.0
16	883.0
17	1486.0
18	2665.0
19	4733.0
20	8921.0
21	15139.0
22	24648.0
23	38136.0
24	56015.0
25	78093.0
26	107343.0
27	143599.0
28	190506.0
29	249457.0
30	323307.0
31	418952.0
32	543488.0
33	723152.0
34	1027796.0
35	1530921.0
36	2297527.0
37	3736661.0
38	8113477.0
39	2.2198757E7
40	13519.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.20922468778575	26.623512196982613	28.11207952340851	25.055183591823127
2	22.14612697187652	26.311332750268623	32.24910208847393	19.29343818938093
3	20.845759746173822	25.252002538949952	33.95671130474061	19.945526410135614
4	21.91037393885053	25.130247348816876	33.13154540179475	19.827833310537837
5	27.022983212670127	25.482605029173932	28.10296166543036	19.391450092725577
6	21.704564537682007	25.048575434407127	32.966017601946284	20.280842425964575
7	27.008550843944708	25.668715680711273	27.587838406788833	19.734895068555186
8	21.984311154988063	25.7440794074662	32.19249042756418	20.07911900998156
9	21.030419992443555	26.273864423475136	32.597844052616786	20.097871531464524
10	21.700306511015004	25.774368266069413	32.40096647169588	20.124358751219702
11	26.98009709447744	25.612470063587487	27.173206489060597	20.234226352874472
12	21.628102608681733	31.307339666019633	26.716030190156214	20.34852753514242
13	24.550529682269012	26.270421204380213	28.061308511188383	21.11774060216239
14	23.712404533516825	26.582264597847765	28.413242109785052	21.292088758850355
15	23.965861044198125	26.612377543501882	28.01347620040271	21.40828521189728
16	23.859985559506057	26.619509615315458	27.923347581475326	21.597157243703155
17	23.623153020931763	26.841215677779257	27.860900905860657	21.67473039542832
18	23.39114921855604	26.774370138749997	28.156532040910786	21.67794860178317
19	23.005785297281466	27.164372754142168	28.497208192132355	21.33263375644401
20	22.64732796162481	27.208993506676592	28.62782916242656	21.515849369272033
21	22.796537349527313	27.23488053355037	28.35749668061469	21.611085436307622
22	22.64688591059597	27.335921888579627	28.47510411615935	21.542088084665053
23	22.78990017101879	27.38046390603171	28.22901550313579	21.600620419813712
24	22.50594051113784	27.486204119415476	28.287757654624436	21.720097714822252
25	22.609775129358468	27.642299093542107	28.08712668073775	21.66079909636168
26	22.598899865509594	27.506463936512844	28.06682788692827	21.827808311049292
27	22.629996654133727	27.561651086794807	27.946201089287126	21.86215116978434
28	22.59323140302005	27.61531998400874	27.9891686467573	21.80227996621391
29	22.72342156114749	27.582298555544433	27.853624711083796	21.84065517222428
30	22.658345784835557	27.51415686340853	27.925336004181755	21.90216134757416
31	22.81939103277903	27.452133090453497	27.748831081839278	21.9796447949282
32	22.823207276748615	27.5541476520844	27.703322814679087	21.9193222564879
33	22.741071021508915	27.503710600940057	27.651561166827605	22.103657210723423
34	22.727138482783378	27.561384639543945	27.68464041371006	22.026836463962614
35	22.800476090307285	27.500349296817177	27.668324804007266	22.03084980886827
36	22.858543125702894	27.462497116127782	27.623372680640916	22.0555870775284
37	22.834210145823935	27.411230366700444	27.64587883235047	22.108680655125156
38	22.787937119791295	27.477090405652326	27.73343939372579	22.001533080830587
39	22.715898739886537	27.351473570821355	27.712015970072745	22.220611719219363
40	22.739057134306954	27.454800162827702	27.73684544548373	22.069297257381606
41	22.794384319211343	27.361003073769563	27.745227853162714	22.099384753856384
42	22.690162595934037	27.4650713960235	27.671153206052896	22.173612801989563
43	22.76040323213661	27.504527741534208	27.602756345916944	22.132312680412237
44	22.801474739579376	27.337876470966616	27.66628932235059	22.19435946710342
45	22.83565573692149	27.292474705091863	27.583437832733416	22.288431725253236
46	22.711829180395338	27.461473938630697	27.5978000864604	22.22889679451356
47	22.72223530056077	27.503734016987952	27.568536308351092	22.205494374100187
48	22.67819268129803	27.335267175163942	27.565848160202734	22.4206919833353
49	22.827214056783056	27.3500149532721	27.568512413700887	22.254258576243956
50	22.675428070269003	27.35434944281977	27.545131498472703	22.425090988438527
51	22.72786705275985	27.386903802420175	27.509411750144192	22.37581739467578
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.26440827960887
2	41.43956516125745
3	40.79128615630944
4	41.73820724938837
5	46.41443330539571
6	41.98540696364658
7	46.743445912499894
8	42.063430164969624
9	41.128291523908075
10	41.8246652622347
11	47.214323447351916
12	41.97663014382415
13	45.6682702844314
14	45.00449329236718
15	45.37414625609541
16	45.457142803209216
17	45.29788341636009
18	45.069097820339216
19	44.33841905372548
20	44.16317733089684
21	44.40762278583493
22	44.18897399526102
23	44.3905205908325
24	44.22603822596009
25	44.270574225720146
26	44.42670817655889
27	44.49214782391807
28	44.39551136923396
29	44.56407673337177
30	44.56050713240972
31	44.79903582770723
32	44.74252953323651
33	44.84472823223234
34	44.75397494674599
35	44.83132589917555
36	44.9141302032313
37	44.94289080094909
38	44.789470200621885
39	44.9365104591059
40	44.80835439168856
41	44.89376907306772
42	44.863775397923604
43	44.892715912548844
44	44.9958342066828
45	45.12408746217472
46	44.940725974908894
47	44.92772967466095
48	45.098884664633324
49	45.08147263302701
50	45.10051905870753
51	45.10368444743563
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44270.0
1	26078.0
2	7886.0
3	10420.0
4	12954.0
5	26933.5
6	40913.0
7	64202.0
8	87491.0
9	63837.0
10	40183.0
11	40058.0
12	39933.0
13	49410.0
14	58887.0
15	80489.0
16	102091.0
17	141637.0
18	181183.0
19	229866.0
20	278549.0
21	338131.5
22	397714.0
23	477453.5
24	557193.0
25	659134.0
26	888224.0
27	1015373.0
28	1118560.0
29	1221747.0
30	1329455.5
31	1437164.0
32	1566115.5
33	1695067.0
34	1817743.0
35	1940419.0
36	2110889.5
37	2281360.0
38	2402447.5
39	2523535.0
40	2643896.5
41	2764258.0
42	2818725.5
43	2873193.0
44	2901861.5
45	2930530.0
46	2902593.0
47	2874656.0
48	2820449.5
49	2766243.0
50	2644498.5
51	2522754.0
52	2356443.0
53	2190132.0
54	2013613.0
55	1837094.0
56	1675216.5
57	1513339.0
58	1338821.0
59	1164303.0
60	1034594.0
61	904885.0
62	786475.0
63	668065.0
64	584070.0
65	500075.0
66	420554.5
67	341034.0
68	327506.5
69	313979.0
70	278919.0
71	243859.0
72	209019.5
73	174180.0
74	147233.5
75	99076.5
76	77866.0
77	74358.0
78	70850.0
79	71707.5
80	72565.0
81	63450.5
82	54336.0
83	49403.5
84	44471.0
85	40590.5
86	36710.0
87	42626.5
88	48543.0
89	30918.5
90	13294.0
91	7899.0
92	2504.0
93	1803.0
94	1102.0
95	639.5
96	177.0
97	124.0
98	71.0
99	50.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27079329187324785
2	0.022437076544982684
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	7.335657613748332E-4
13	0.0
14	5.089560494229299E-4
15	0.0
16	7.168395062294787E-6
17	1.4336790124589574E-5
18	6.7382913585571E-4
19	0.0053046123460981425
20	0.0
21	7.168395062294787E-6
22	0.0
23	0.0
24	4.062090535300379E-5
25	0.0
26	0.004860171852235865
27	0.004105100905674148
28	3.5841975311473935E-5
29	4.778930041529858E-6
30	0.0
31	0.003393040329486199
32	0.002179192098937615
33	1.9115720166119433E-5
34	0.0
35	0.004800435226716742
36	0.0030035575311015156
37	2.6045168726337726E-4
38	4.778930041529858E-6
39	2.2222024693113838E-4
40	0.0
41	3.1063045269944076E-4
42	4.778930041529858E-6
43	8.841020576830237E-5
44	0.0
45	1.242521810797763E-4
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	6.212609053988815E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.1850372E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.43297796796606
#Duplication Level	Relative count
1	100.0
2	12.544477549114395
3	3.685799412008445
4	1.7613438303637787
5	1.0924541083772354
6	0.7524187236505462
7	0.5715488381576265
8	0.4544766940931184
9	0.36173977098583954
10++	10.317475977190663
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47046	0.11241477136690685	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	14937555	4.024978	5.0911965	18
TGTGT	9253835	3.871551	49.941017	4
AAAAA	9448660	3.3222208	3.5052342	44
TGTTT	8372725	2.8111916	40.084923	6
GTTTG	6634605	2.7757368	49.224648	7
CTTTG	5955100	2.6462073	52.18934	1
GTGTT	6219880	2.602227	49.22366	5
TTTGA	7288115	2.5807958	41.906406	8
TTGTG	5535170	2.3157635	49.203903	3
TTTGT	6833435	2.2943656	39.689518	2
GAGGG	3126145	2.141738	13.117005	11
GGGGG	2572910	2.0826035	5.2831845	13
TTGAG	4534990	2.0010338	29.940855	9
TGAGG	3492775	1.9203848	17.635654	10
AGGGG	2268220	1.5539691	7.1775756	12
TGAGC	2493530	1.4561424	6.6169405	10
TGAGA	3118055	1.4510309	5.5482755	10
TGAGT	2862945	1.2632551	8.5103445	10
GAGTG	2295780	1.2622573	6.5510173	11
TTGAT	3188375	1.1290362	9.583297	9
TTGAC	2121275	0.994138	8.783105	9
>>END_MODULE