FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | 140701_JD_C_5_CTTGTA_L002_R2_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 25970033 |
| Filtered Sequences | 0 |
| Sequence length | 51 |
| %GC | 43 |
Per base sequence quality
Per sequence quality scores
![[WARN]](Icons/warning.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 122613 | 0.4721326307132533 | No Hit |
| CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 34947 | 0.13456663686180145 | No Hit |
![[FAIL]](Icons/error.png)
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TGTGT | 8370710 | 5.034502 | 52.347076 | 4 |
| TTTTT | 12901255 | 4.821129 | 6.463686 | 18 |
| GTTTG | 6396255 | 3.8469803 | 51.800713 | 7 |
| AAAAA | 6287055 | 3.8317115 | 4.223701 | 43 |
| GTGTT | 6082900 | 3.6585155 | 51.761925 | 5 |
| TGTTT | 7433315 | 3.524017 | 41.304386 | 6 |
| TTTGA | 6711930 | 3.5090425 | 45.187202 | 8 |
| TTGTG | 5537710 | 3.3306148 | 51.816727 | 3 |
| CTTTG | 4845840 | 3.2650907 | 58.084393 | 1 |
| GGGGG | 2240320 | 2.751188 | 6.7017365 | 13 |
| TTGAG | 4052300 | 2.6877046 | 33.08751 | 9 |
| TTTGT | 5516595 | 2.6153305 | 41.017704 | 2 |
| GAGGG | 2353045 | 2.511814 | 15.0423975 | 11 |
| TGAGG | 2709705 | 2.28003 | 19.34243 | 10 |
| GAGAG | 2431875 | 2.2565534 | 5.604702 | 11 |
| ACTTT | 3101005 | 1.8162506 | 22.273726 | 3 |
| AACTT | 2797235 | 1.8067081 | 25.335318 | 2 |
| GAGGC | 1509525 | 1.8052179 | 5.002151 | 11 |
| AGGGG | 1673280 | 1.7861828 | 8.195719 | 12 |
| TGAGC | 1645270 | 1.5509142 | 7.596893 | 10 |
| TGAGA | 2062200 | 1.5083301 | 6.237604 | 10 |
| TAACT | 2223305 | 1.436012 | 25.217207 | 1 |
| TGAGT | 2077915 | 1.3781855 | 9.380818 | 10 |
| GAGTG | 1603830 | 1.3495126 | 7.3206463 | 11 |
| TTGAT | 2506650 | 1.3104937 | 10.42405 | 9 |
| TTGAC | 1602190 | 1.1904906 | 9.610065 | 9 |
| GTAAC | 1006360 | 0.82461464 | 8.083408 | 1 |