##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_5_CTTGTA_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25970033	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38580540117142	34.0	31.0	34.0	31.0	34.0
2	32.517309854785324	34.0	31.0	34.0	31.0	34.0
3	32.595749839825004	34.0	31.0	34.0	31.0	34.0
4	35.92766551355557	37.0	37.0	37.0	35.0	37.0
5	35.95393432884741	37.0	37.0	37.0	35.0	37.0
6	35.946449201662546	37.0	37.0	37.0	35.0	37.0
7	35.97579972270347	37.0	37.0	37.0	35.0	37.0
8	35.95616104916001	37.0	37.0	37.0	35.0	37.0
9	37.72893850385173	39.0	39.0	39.0	35.0	39.0
10	37.707414156924635	39.0	39.0	39.0	35.0	39.0
11	37.71518877161227	39.0	39.0	39.0	37.0	39.0
12	37.662722261461894	39.0	39.0	39.0	35.0	39.0
13	37.676449082679255	39.0	39.0	39.0	35.0	39.0
14	39.19260091044166	41.0	40.0	41.0	37.0	41.0
15	39.143741519311895	41.0	40.0	41.0	37.0	41.0
16	39.12454077359085	41.0	40.0	41.0	36.0	41.0
17	39.101881233651106	41.0	40.0	41.0	36.0	41.0
18	39.07052736513658	41.0	40.0	41.0	36.0	41.0
19	39.070569683142104	41.0	40.0	41.0	36.0	41.0
20	39.0425298650949	41.0	40.0	41.0	36.0	41.0
21	39.00829059400887	41.0	40.0	41.0	36.0	41.0
22	38.96381864435829	41.0	39.0	41.0	36.0	41.0
23	38.94258274527414	41.0	39.0	41.0	36.0	41.0
24	38.909886675923744	41.0	39.0	41.0	36.0	41.0
25	38.84268645326712	41.0	39.0	41.0	36.0	41.0
26	38.801287699557406	41.0	39.0	41.0	35.0	41.0
27	38.750979638724374	41.0	39.0	41.0	35.0	41.0
28	38.71523590285773	41.0	39.0	41.0	35.0	41.0
29	38.665133810188074	41.0	39.0	41.0	35.0	41.0
30	38.62840574750136	41.0	39.0	41.0	35.0	41.0
31	38.555854357212404	41.0	39.0	41.0	35.0	41.0
32	38.518080550764026	41.0	39.0	41.0	35.0	41.0
33	38.44381580108119	40.0	39.0	41.0	35.0	41.0
34	38.42404859477845	40.0	38.0	41.0	35.0	41.0
35	38.361978092211125	40.0	38.0	41.0	34.0	41.0
36	38.31104226937255	40.0	38.0	41.0	34.0	41.0
37	38.26307479085606	40.0	38.0	41.0	34.0	41.0
38	38.21340612081625	40.0	38.0	41.0	34.0	41.0
39	38.15673742116539	40.0	38.0	41.0	34.0	41.0
40	38.09320727470774	40.0	38.0	41.0	34.0	41.0
41	38.03165317502677	40.0	38.0	41.0	33.0	41.0
42	37.95591291701478	40.0	38.0	41.0	33.0	41.0
43	37.90202642407116	40.0	38.0	41.0	33.0	41.0
44	37.83023452453834	40.0	38.0	41.0	33.0	41.0
45	37.74381746068632	40.0	38.0	41.0	33.0	41.0
46	37.68556335681206	40.0	38.0	41.0	33.0	41.0
47	37.62021087920836	40.0	38.0	41.0	33.0	41.0
48	37.79970121716826	40.0	38.0	41.0	33.0	41.0
49	37.85999378591471	40.0	38.0	41.0	33.0	41.0
50	37.823261949647886	40.0	38.0	41.0	33.0	41.0
51	36.31345555086511	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	130392.0
3	3218.0
4	2238.0
5	1430.0
6	1616.0
7	3711.0
8	10942.0
9	17303.0
10	16971.0
11	17356.0
12	20386.0
13	24918.0
14	28913.0
15	31540.0
16	32763.0
17	33702.0
18	34507.0
19	35776.0
20	37601.0
21	40364.0
22	43912.0
23	50522.0
24	56877.0
25	66267.0
26	78782.0
27	91141.0
28	106442.0
29	127386.0
30	156095.0
31	194976.0
32	252336.0
33	339550.0
34	497330.0
35	781963.0
36	1212723.0
37	1943498.0
38	4335116.0
39	1.5099544E7
40	9926.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.625799326156116	24.60605124535384	32.25232822297542	24.515821205514623
2	20.667820320662713	27.622891964094382	34.57148669979393	17.137801015448975
3	22.52543151594931	26.963022124268726	32.59855172297349	17.912994636808474
4	20.5991403639267	23.560606078209982	34.954335464414704	20.88591809344861
5	29.248000351933722	23.22917468598977	29.41923500575687	18.103589956319635
6	19.972205187544127	22.813510763133014	38.88200532153105	18.332278727791813
7	26.58598153066809	23.351416864658567	32.35541557700458	17.707186027668765
8	23.100292562375483	23.64435925487617	35.28857482396652	17.966773358781822
9	23.178134230699644	24.26604788306836	34.55839595333368	17.997421932898316
10	22.96672150275138	26.679347995287017	32.285825230928786	18.06810527103281
11	28.59095155474312	23.779117597445136	29.04298579207006	18.586945055741687
12	21.261129954885146	30.635541261696726	29.318703558325854	18.78462522509227
13	25.208527848417962	24.456135533046268	30.543362964983395	19.791973653552375
14	26.505957903493517	25.18149107897053	28.210247052738506	20.10230396479745
15	24.70393802317191	27.810102556333486	27.16565172980745	20.320307690687148
16	25.383041990737134	25.934359062740846	27.879949019739463	20.802649926782557
17	24.825887379528567	26.042584556006286	28.310802857488014	20.82072520697713
18	24.484025988855837	25.999496790101475	28.544535043730875	20.971942177311814
19	23.94103665648821	26.481284812291307	28.9427696356647	20.63490889555578
20	23.40642306022711	26.595582481262127	29.093182413367963	20.904812045142805
21	23.43351173609326	26.792780444712317	28.886752294879713	20.88695552431471
22	22.97336435614578	27.102949918677254	29.211790502199175	20.711895222977788
23	22.95545943154023	27.259979661812604	28.990974464860734	20.79358644178643
24	22.72051784624293	27.394298237021808	28.929189031269882	20.955994885465383
25	22.74247764847371	27.598887104369897	28.724353604839354	20.934281642317043
26	22.691777494278064	27.53827894696348	28.747787226319726	21.02215633243873
27	22.707808572522424	27.580558904073094	28.57163103414187	21.140001489262612
28	22.624545838792503	27.663961208787814	28.56689814856922	21.144594803850467
29	22.786107421350923	27.580219577401778	28.388627844352666	21.245045156894633
30	22.741742438409783	27.58330162642228	28.36404068151852	21.31091525364942
31	22.83046757408535	27.550754449849073	28.237953512988607	21.380824463076966
32	22.8772890606563	27.61828890519832	28.118096954281995	21.38632507986339
33	22.84370162871146	27.571160663908213	28.088757528638553	21.496380178741777
34	22.857638946690724	27.59110806218518	28.076079563029726	21.47517342809436
35	22.948026876695376	27.552732389174672	27.969414865813985	21.529825868315964
36	22.84733158135009	27.55536384499788	28.05145363368733	21.545850939964694
37	22.84444911112998	27.4549279289398	28.076927678286133	21.623695281644085
38	22.790229101947112	27.509183548195253	28.164193606823385	21.536393743034253
39	22.766057484633283	27.402348690125596	28.125968004530055	21.705625820711063
40	22.78021505056688	27.473648016159043	28.12274100346738	21.623395929806698
41	22.848119590874546	27.423613974541677	28.090583868584883	21.637682565998894
42	22.709505415616583	27.443018908027778	28.115555823391226	21.731919852964417
43	22.821895043022984	27.492034085149697	27.999255786814974	21.68681508501234
44	22.849106122678798	27.382496282659023	28.047996934273133	21.720400660389043
45	22.84133593237817	27.446158913908274	27.9372024576143	21.77530269609926
46	22.69617043394975	27.53189627160247	27.912706995093668	21.859226299354116
47	22.789729121391204	27.436430567719032	27.97324500077379	21.800595310115977
48	22.697177797579194	27.385076466861907	27.877262224612405	22.040483510946498
49	22.74156293575917	27.434042841729966	27.912828281345803	21.911565941165065
50	22.638984709017805	27.4705458303503	27.930295283646338	21.960174176985554
51	22.70461945266205	27.42555801082689	27.87957182466021	21.99025071185085
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	43.14162053167074
2	37.80562133611169
3	40.438426152757785
4	41.48505845737531
5	47.35159030825336
6	38.30448391533594
7	44.29316755833685
8	41.0670659211573
9	41.17555616359796
10	41.03482677378419
11	47.1778966104848
12	40.04575517997742
13	45.000501501970334
14	46.60826186829096
15	45.02424571385906
16	46.18569191751969
17	45.6466125865057
18	45.45596816616765
19	44.575945552044
20	44.31123510536991
21	44.320467260407966
22	43.68525957912357
23	43.74904587332666
24	43.676512731708314
25	43.67675929079075
26	43.7139338267168
27	43.84781006178503
28	43.769140642642974
29	44.03115257824556
30	44.052657692059206
31	44.21129203716232
32	44.263614140519685
33	44.34008180745324
34	44.33281237478509
35	44.47785274501134
36	44.393182521314785
37	44.468144392774064
38	44.326622844981365
39	44.47168330534435
40	44.40361098037358
41	44.48580215687344
42	44.441425268581
43	44.508710128035325
44	44.56950678306784
45	44.61663862847743
46	44.555396733303866
47	44.590324431507184
48	44.73766130852569
49	44.65312887692423
50	44.59915888600335
51	44.6948701645129
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152670.0
1	81262.5
2	9855.0
3	10676.0
4	11497.0
5	36796.0
6	62095.0
7	77005.5
8	91916.0
9	66034.5
10	40153.0
11	35475.5
12	30798.0
13	35301.5
14	39805.0
15	51987.0
16	64169.0
17	85563.0
18	106957.0
19	135789.5
20	164622.0
21	200787.5
22	236953.0
23	286076.5
24	335200.0
25	396360.0
26	537803.5
27	618087.0
28	691085.5
29	764084.0
30	840620.5
31	917157.0
32	1006278.0
33	1095399.0
34	1174425.0
35	1253451.0
36	1358895.5
37	1464340.0
38	1542958.5
39	1621577.0
40	1691426.5
41	1761276.0
42	1798223.0
43	1835170.0
44	1848710.5
45	1862251.0
46	1834649.5
47	1807048.0
48	1754878.0
49	1702708.0
50	1617578.0
51	1532448.0
52	1416241.0
53	1300034.0
54	1189337.5
55	1078641.0
56	975381.5
57	872122.0
58	769924.5
59	667727.0
60	591693.5
61	515660.0
62	445373.5
63	375087.0
64	324901.0
65	274715.0
66	231860.5
67	189006.0
68	176748.5
69	164491.0
70	146178.0
71	127865.0
72	110326.0
73	92787.0
74	77235.0
75	51490.5
76	41298.0
77	38376.0
78	35454.0
79	35660.0
80	35866.0
81	31509.0
82	27152.0
83	24349.0
84	21546.0
85	19054.0
86	16562.0
87	18871.5
88	21181.0
89	14278.5
90	7376.0
91	4785.0
92	2194.0
93	1732.5
94	1271.0
95	982.0
96	693.0
97	506.0
98	319.0
99	208.0
100	97.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7757133000177551
2	0.5977851472117883
3	0.5673808731779432
4	0.5750435511575976
5	0.5659946600760962
6	0.553064372309423
7	0.551477928426198
8	0.5232338364760646
9	0.5473847491837996
10	0.5590635945668609
11	0.5659946600760962
12	0.5372153358449718
13	0.5299646712039219
14	0.5464144000125067
15	0.5688749028543784
16	0.5316666328456341
17	0.5204999161918663
18	0.523229985884115
19	0.5566569745983766
20	0.5452322682839872
21	0.5660447177714406
22	0.5920747193505684
23	0.5194024974862373
24	0.5425445551031838
25	0.540919605300463
26	0.5560793858059403
27	0.5589981345037182
28	0.5083397468151081
29	0.5662911556562135
30	0.5278160408960589
31	0.5585514658375675
32	0.5510890186392909
33	0.5461063526565407
34	0.5369380932246024
35	0.5485283749928235
36	0.5547278280316393
37	0.5451128999335504
38	0.5427948435799061
39	0.5284975956711337
40	0.5230259045107875
41	0.535355499933327
42	0.577161376729864
43	0.5341733682048074
44	0.5639846510784179
45	0.5451283023013487
46	0.553780582412044
47	0.5486554445271594
48	0.5615703299260344
49	0.5643658596814258
50	0.5628987841486378
51	0.5195064634688759
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5970033E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.47894988245651
#Duplication Level	Relative count
1	100.0
2	11.50329329717164
3	3.3875758749838565
4	1.67441560118817
5	1.0312540359033966
6	0.7419604804339404
7	0.5327392483533514
8	0.4487924577037324
9	0.3945499160532093
10++	9.434327779930259
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	122613	0.4721326307132533	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34947	0.13456663686180145	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	8370710	5.034502	52.347076	4
TTTTT	12901255	4.821129	6.463686	18
GTTTG	6396255	3.8469803	51.800713	7
AAAAA	6287055	3.8317115	4.223701	43
GTGTT	6082900	3.6585155	51.761925	5
TGTTT	7433315	3.524017	41.304386	6
TTTGA	6711930	3.5090425	45.187202	8
TTGTG	5537710	3.3306148	51.816727	3
CTTTG	4845840	3.2650907	58.084393	1
GGGGG	2240320	2.751188	6.7017365	13
TTGAG	4052300	2.6877046	33.08751	9
TTTGT	5516595	2.6153305	41.017704	2
GAGGG	2353045	2.511814	15.0423975	11
TGAGG	2709705	2.28003	19.34243	10
GAGAG	2431875	2.2565534	5.604702	11
ACTTT	3101005	1.8162506	22.273726	3
AACTT	2797235	1.8067081	25.335318	2
GAGGC	1509525	1.8052179	5.002151	11
AGGGG	1673280	1.7861828	8.195719	12
TGAGC	1645270	1.5509142	7.596893	10
TGAGA	2062200	1.5083301	6.237604	10
TAACT	2223305	1.436012	25.217207	1
TGAGT	2077915	1.3781855	9.380818	10
GAGTG	1603830	1.3495126	7.3206463	11
TTGAT	2506650	1.3104937	10.42405	9
TTGAC	1602190	1.1904906	9.610065	9
GTAAC	1006360	0.82461464	8.083408	1
>>END_MODULE