FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_5_CTTGTA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_5_CTTGTA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 25970033
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42372 0.16315728208739666 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11513670 4.6887984 6.0177817 18
TGTGT 6253920 4.081467 56.709526 4
AAAAA 6421425 3.6359248 3.9467196 47
GTTTG 4646250 3.0322611 56.07625 7
TGTTT 5700115 2.9385939 44.831554 6
GTGTT 4307190 2.8109818 56.012276 5
CTTTG 4005585 2.807797 59.85662 1
TTTGA 5006880 2.7570853 47.532593 8
TTGTG 3800795 2.4804952 55.96377 3
TTTGT 4608940 2.3760576 44.374184 2
GAGGG 2003365 2.2380378 15.26909 11
TTGAG 3062570 2.134898 34.327843 9
GGGGG 1610585 2.1324162 5.94606 13
GAGAG 2237740 2.1092887 5.472687 11
TGAGG 2278415 2.0106297 20.233246 10
AGGGG 1439320 1.6079209 8.3236065 12
TGAGC 1547125 1.4664266 7.600314 10
TGAGA 1948410 1.4507703 6.3999257 10
TGAGT 1835665 1.2796304 9.702513 10
GAGTG 1443510 1.2738522 7.464317 11
TGATG 1721650 1.2001513 5.1136723 10
TTGAT 2130060 1.1729374 11.256774 9
TTGAC 1395225 1.0446506 9.863699 9
TGATT 1775980 0.97796005 5.4219375 10
Produced by FastQC (version 0.10.1)