##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_5_CTTGTA_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	25970033	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99559607798727	34.0	33.0	34.0	31.0	34.0
2	33.181178630000204	34.0	34.0	34.0	31.0	34.0
3	33.21772941143355	34.0	34.0	34.0	31.0	34.0
4	36.53321784381252	37.0	37.0	37.0	35.0	37.0
5	36.47045519734226	37.0	37.0	37.0	35.0	37.0
6	36.43785697153331	37.0	37.0	37.0	35.0	37.0
7	36.45819560568136	37.0	37.0	37.0	35.0	37.0
8	36.42258452270738	37.0	37.0	37.0	35.0	37.0
9	38.25805696896881	39.0	39.0	39.0	37.0	39.0
10	38.25257299442015	39.0	39.0	39.0	37.0	39.0
11	38.24446233857308	39.0	39.0	39.0	37.0	39.0
12	38.19694410861935	39.0	39.0	39.0	37.0	39.0
13	38.1736511463039	39.0	39.0	39.0	37.0	39.0
14	39.72190231718227	41.0	40.0	41.0	38.0	41.0
15	39.70179502659854	41.0	40.0	41.0	38.0	41.0
16	39.68117117910478	41.0	40.0	41.0	37.0	41.0
17	39.64974438037872	41.0	40.0	41.0	37.0	41.0
18	39.63486114938706	41.0	40.0	41.0	37.0	41.0
19	39.62536408790855	41.0	40.0	41.0	37.0	41.0
20	39.55411908795033	41.0	40.0	41.0	37.0	41.0
21	39.5455915670188	41.0	40.0	41.0	37.0	41.0
22	39.490004267611056	41.0	39.0	41.0	37.0	41.0
23	39.47578534074254	41.0	39.0	41.0	37.0	41.0
24	39.461377696362575	41.0	39.0	41.0	37.0	41.0
25	39.42192899793389	41.0	39.0	41.0	36.0	41.0
26	39.31828480926458	41.0	39.0	41.0	36.0	41.0
27	39.26127899028854	41.0	39.0	41.0	36.0	41.0
28	39.20830547269617	41.0	39.0	41.0	36.0	41.0
29	39.16600140631319	40.0	39.0	41.0	36.0	41.0
30	39.0804710182694	40.0	39.0	41.0	36.0	41.0
31	39.02011660901625	40.0	39.0	41.0	35.0	41.0
32	38.9461273691874	40.0	39.0	41.0	35.0	41.0
33	38.86602731694642	40.0	38.0	41.0	35.0	41.0
34	38.79984753966235	40.0	38.0	41.0	35.0	41.0
35	38.73010511769469	40.0	38.0	41.0	35.0	41.0
36	38.66516623217229	40.0	38.0	41.0	35.0	41.0
37	38.84282056938472	40.0	38.0	41.0	35.0	41.0
38	38.861042879691375	40.0	38.0	41.0	35.0	41.0
39	38.80706528174223	40.0	38.0	41.0	35.0	41.0
40	38.77461792212586	40.0	38.0	41.0	35.0	41.0
41	38.74563655733514	40.0	38.0	41.0	35.0	41.0
42	38.68701060179631	40.0	38.0	41.0	35.0	41.0
43	38.64445774866747	40.0	38.0	41.0	35.0	41.0
44	38.57528459821364	40.0	38.0	41.0	35.0	41.0
45	38.52357626961814	40.0	38.0	41.0	35.0	41.0
46	38.45376315078229	40.0	38.0	41.0	35.0	41.0
47	38.35534225158666	40.0	38.0	41.0	34.0	41.0
48	38.308142927658196	40.0	38.0	41.0	34.0	41.0
49	38.26339192560903	40.0	38.0	41.0	34.0	41.0
50	38.36127686091119	40.0	38.0	41.0	34.0	41.0
51	38.09712486695724	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	10.0
11	25.0
12	59.0
13	114.0
14	236.0
15	389.0
16	630.0
17	1104.0
18	1721.0
19	2966.0
20	5010.0
21	8385.0
22	13471.0
23	20476.0
24	29672.0
25	40982.0
26	55260.0
27	73361.0
28	95608.0
29	124296.0
30	160892.0
31	206438.0
32	267034.0
33	358486.0
34	517476.0
35	811956.0
36	1245362.0
37	2043060.0
38	4603715.0
39	1.5266576E7
40	15255.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.875572540087695	26.257639383032256	28.27190227887655	25.594885798003503
2	21.949333729937784	26.009055767417678	33.32793676058834	18.713673742056198
3	20.549541249960292	24.99124663915418	35.17875319719561	19.28045891368992
4	21.66612546155016	24.8395204572261	34.17271917276596	19.32163490845778
5	27.63880468922314	25.190461954278348	28.32962444073966	18.84110891575885
6	21.437015501674566	24.649814653681805	34.16371477078985	19.749455073853774
7	27.758296775167373	25.399477782659474	27.67066499345592	19.171560448717234
8	21.800153276663146	25.542932502242106	33.165941683632056	19.490972537462696
9	20.773997476245025	26.093151287100792	33.57670743044493	19.556143806209256
10	21.47691231185237	25.466221990022962	33.43815286789019	19.61871283023448
11	27.776661958663006	25.315495287632327	27.193823171079202	19.714019582625465
12	21.41361296461672	32.030868069043336	26.638170461865084	19.917348504474855
13	24.86110433513889	26.08280859712423	28.28549351477528	20.7705935529616
14	23.75598444561083	26.495923205026344	28.726343936490185	21.021748412872636
15	24.110385227465827	26.525206956802865	28.217457405618234	21.14695041011307
16	23.967669625015475	26.507118500747307	28.1383734812527	21.386838392984522
17	23.76776340638458	26.783855068647778	28.049721769702796	21.39865975526485
18	23.41435222666063	26.663543323183305	28.46902812945983	21.453076320696244
19	22.925270188845705	27.149558505696426	28.825889919685743	21.09928138577213
20	22.537008443004417	27.257194744290302	28.991241164121963	21.214555648583318
21	22.735317728140203	27.247860460873337	28.692164170555333	21.324657640431123
22	22.55237796578849	27.39536372556785	28.767483660879446	21.284774647764213
23	22.6705641844968	27.430835378607338	28.57086858534219	21.327731851553676
24	22.3819122601808	27.52475901744137	28.597453072162054	21.49587565021577
25	22.48257163049662	27.683955041379647	28.446881083699072	21.386592244424666
26	22.485555750181664	27.597934741913782	28.342362268989906	21.57414723891465
27	22.510330851793107	27.611842683105415	28.228268688361396	21.649557776740085
28	22.458107855425933	27.706824606708103	28.275946144678187	21.559121393187777
29	22.61738466692925	27.640807249188803	28.153871869886295	21.58793621399565
30	22.507461435946592	27.58501158122063	28.249583535447787	21.65794344738499
31	22.635676549952827	27.547915043551814	28.030628261495792	21.785780144999563
32	22.664939652172187	27.696059876270763	27.959671282452664	21.67932918910439
33	22.606342834153665	27.577357815766273	27.944143019352197	21.87215633072786
34	22.562422754552305	27.649547013460968	27.93627040948092	21.851759822505812
35	22.6601762180219	27.57804803515242	27.9595703415002	21.802205405325477
36	22.70281568200893	27.563801327047273	27.93205933221912	21.801323658724684
37	22.776292804795993	27.48449641612415	27.88814815450909	21.851062624570766
38	22.604480722877	27.546158441395818	28.064837566845384	21.7845232688818
39	22.608042123011547	27.546356987686536	27.90186288943106	21.94373799987085
40	22.600506489456325	27.599724617969812	27.94701341072289	21.852755481850973
41	22.64291890395356	27.43849655090887	28.031209995731594	21.887374549405976
42	22.53513967787305	27.55452871292657	27.927122411514244	21.983209197686136
43	22.61288992137893	27.599379915549516	27.859918888752105	21.927811274319446
44	22.659334243641815	27.438049459546537	27.912844348454875	21.989771948356776
45	22.746307753751775	27.385079331291685	27.81723082648064	22.051382088475904
46	22.557107537202995	27.537143695914416	27.886123932766605	22.01962483411598
47	22.57407567777456	27.62820172438005	27.82226003675237	21.97546256109302
48	22.525472749039178	27.42856636776947	27.84647309135842	22.199487791832933
49	22.725559205079726	27.435473374945612	27.819017400914138	22.019950019060527
50	22.534593214007632	27.470248956083964	27.7859545954235	22.209203234484907
51	22.600452279826648	27.476051453174303	27.73910800363244	22.184388263366607
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.4704583380912
2	40.663007471993986
3	39.83000016365021
4	40.98776037000794
5	46.479913604981995
6	41.18647057552834
7	46.92985722388461
8	41.29112581412585
9	40.33014128245428
10	41.09562514208685
11	47.490681541288474
12	41.33096146909158
13	45.63169788810049
14	44.77773285848347
15	45.2573356375789
16	45.354508018
17	45.166423161649426
18	44.867428547356866
19	44.02455157461784
20	43.75156409158774
21	44.05997536857133
22	43.8371526135527
23	43.99829603605047
24	43.87778791039657
25	43.869163874921284
26	44.05970298909631
27	44.159888628533196
28	44.01722924861371
29	44.2053208809249
30	44.16540488333159
31	44.4214566949524
32	44.34426884127657
33	44.478499164881526
34	44.414182577058114
35	44.46238162334738
36	44.50413934073361
37	44.62735542936676
38	44.3890039917588
39	44.5517801228824
40	44.4532619713073
41	44.53029345335954
42	44.51834887555919
43	44.54070119569838
44	44.64910619199859
45	44.797689842227676
46	44.576732371318975
47	44.549538238867584
48	44.72496054087211
49	44.74550922414026
50	44.74379644849254
51	44.784840543193255
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42424.0
1	24801.0
2	7178.0
3	9271.0
4	11364.0
5	23249.0
6	35134.0
7	56480.5
8	77827.0
9	55703.5
10	33580.0
11	31146.5
12	28713.0
13	34281.5
14	39850.0
15	54084.5
16	68319.0
17	92671.5
18	117024.0
19	147669.5
20	178315.0
21	215404.5
22	252494.0
23	303269.0
24	354044.0
25	417134.0
26	561052.5
27	641881.0
28	707788.5
29	773696.0
30	843165.0
31	912634.0
32	995068.0
33	1077502.0
34	1152493.0
35	1227484.0
36	1329662.0
37	1431840.0
38	1505054.0
39	1578268.0
40	1647724.5
41	1717181.0
42	1754751.5
43	1792322.0
44	1808944.5
45	1825567.0
46	1801684.0
47	1777801.0
48	1737964.5
49	1698128.0
50	1626011.0
51	1553894.0
52	1441902.0
53	1329910.0
54	1223838.5
55	1117767.0
56	1014369.5
57	910972.0
58	807958.5
59	704945.0
60	626459.5
61	547974.0
62	473990.5
63	400007.0
64	346508.0
65	293009.0
66	248885.0
67	204761.0
68	193653.5
69	182546.0
70	162529.0
71	142512.0
72	123157.5
73	103803.0
74	86366.5
75	56518.5
76	44107.0
77	41399.0
78	38691.0
79	39472.5
80	40254.0
81	35443.5
82	30633.0
83	27028.5
84	23424.0
85	20839.0
86	18254.0
87	21445.0
88	24636.0
89	15614.5
90	6593.0
91	3819.5
92	1046.0
93	743.0
94	440.0
95	268.5
96	97.0
97	57.5
98	18.0
99	17.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21864431208077403
2	0.011301487372002953
3	3.0804735596600896E-5
4	8.163254933099237E-4
5	6.546006314277691E-5
6	0.0
7	1.1551775848725338E-5
8	0.0
9	0.0
10	3.850591949575112E-6
11	2.810932123189832E-4
12	9.62647987393778E-5
13	0.0
14	0.0
15	0.0
16	1.0781657458810314E-4
17	0.0
18	0.0
19	1.8867900552918052E-4
20	0.0018713876874935044
21	0.002972656985071987
22	0.0
23	0.0
24	0.0
25	7.316124704192713E-5
26	3.8505919495751124E-5
27	2.3103551697450676E-5
28	3.4655327546176006E-5
29	3.8505919495751124E-5
30	0.003219094869844794
31	6.237958958311682E-4
32	0.002876392186332609
33	2.502884767223823E-4
34	2.156331491762063E-4
35	0.0014824779005864182
36	7.585666140662971E-4
37	0.005236805051422153
38	1.0396598263852803E-4
39	0.0
40	0.0011243728492759328
41	5.698876085371166E-4
42	0.0017635711129054012
43	8.471302289065247E-5
44	3.157485398651592E-4
45	7.893713496628981E-4
46	3.118979479155841E-4
47	1.5402367798300448E-5
48	4.7362280979773883E-4
49	5.1212872929349E-4
50	0.0010473610102844306
51	0.00274162146809748
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5970033E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.07450806470437
#Duplication Level	Relative count
1	100.0
2	11.70976451956279
3	3.4952099073136536
4	1.6869174650154477
5	1.0586234649635227
6	0.7717371550224577
7	0.5744216839317704
8	0.48095646078355014
9	0.3790534049900044
10++	9.656125866500506
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42372	0.16315728208739666	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11513670	4.6887984	6.0177817	18
TGTGT	6253920	4.081467	56.709526	4
AAAAA	6421425	3.6359248	3.9467196	47
GTTTG	4646250	3.0322611	56.07625	7
TGTTT	5700115	2.9385939	44.831554	6
GTGTT	4307190	2.8109818	56.012276	5
CTTTG	4005585	2.807797	59.85662	1
TTTGA	5006880	2.7570853	47.532593	8
TTGTG	3800795	2.4804952	55.96377	3
TTTGT	4608940	2.3760576	44.374184	2
GAGGG	2003365	2.2380378	15.26909	11
TTGAG	3062570	2.134898	34.327843	9
GGGGG	1610585	2.1324162	5.94606	13
GAGAG	2237740	2.1092887	5.472687	11
TGAGG	2278415	2.0106297	20.233246	10
AGGGG	1439320	1.6079209	8.3236065	12
TGAGC	1547125	1.4664266	7.600314	10
TGAGA	1948410	1.4507703	6.3999257	10
TGAGT	1835665	1.2796304	9.702513	10
GAGTG	1443510	1.2738522	7.464317	11
TGATG	1721650	1.2001513	5.1136723	10
TTGAT	2130060	1.1729374	11.256774	9
TTGAC	1395225	1.0446506	9.863699	9
TGATT	1775980	0.97796005	5.4219375	10
>>END_MODULE