FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_4_CCGTCC_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_4_CCGTCC_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 42321153
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 175489 0.4146602527582365 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 22857510 5.6388383 6.9137425 17
TGTGT 9739950 3.9403353 23.372046 7
AAAAA 11803510 3.7146199 4.095002 44
GGGGG 3722410 3.162454 6.3249173 16
GTTTG 7587760 3.0696583 22.97377 10
TGTTT 9252720 2.923062 18.441471 9
GTGTT 6944425 2.8093946 22.742191 8
TTTGA 7989730 2.6500201 18.984821 11
CTTTG 5905575 2.6072896 24.337025 4
TTGTG 5979710 2.4191153 22.596882 6
GAGGG 3364925 2.343776 8.5949 14
TTTGT 6841060 2.161185 17.622334 5
TTGAG 5078455 2.1570318 14.895587 12
TGAGG 3814425 2.0747313 9.838152 13
AGGGG 2663790 1.8554133 5.401248 15
ACTTT 5072465 1.8360596 20.018448 3
AACTT 4790125 1.8203843 21.52238 2
TAACT 3631045 1.3799008 21.23985 1
GTAAC 1731770 0.8427801 8.514735 1
Produced by FastQC (version 0.10.1)