##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_4_CCGTCC_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	42321153	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.154178360877836	34.0	31.0	34.0	31.0	34.0
2	32.32184550832063	34.0	31.0	34.0	31.0	34.0
3	32.389826170378676	34.0	31.0	34.0	31.0	34.0
4	35.78490345005487	37.0	37.0	37.0	35.0	37.0
5	35.80518217450267	37.0	37.0	37.0	35.0	37.0
6	35.82672421519329	37.0	37.0	37.0	35.0	37.0
7	35.833171204007606	37.0	37.0	37.0	35.0	37.0
8	35.83535238276708	37.0	37.0	37.0	35.0	37.0
9	37.55630932361413	39.0	38.0	39.0	35.0	39.0
10	37.54101524596931	39.0	38.0	39.0	35.0	39.0
11	37.536888468043394	39.0	38.0	39.0	35.0	39.0
12	37.5019434607559	39.0	38.0	39.0	35.0	39.0
13	37.49530878801908	39.0	38.0	39.0	35.0	39.0
14	38.98724807899256	41.0	40.0	41.0	36.0	41.0
15	38.935955620112715	41.0	39.0	41.0	36.0	41.0
16	38.913889137188676	41.0	39.0	41.0	36.0	41.0
17	38.86131968096427	41.0	39.0	41.0	36.0	41.0
18	38.832398753408256	41.0	39.0	41.0	36.0	41.0
19	38.83545353785612	41.0	39.0	41.0	36.0	41.0
20	38.798364472725964	41.0	39.0	41.0	36.0	41.0
21	38.751739348878324	41.0	39.0	41.0	35.0	41.0
22	38.71008925489341	41.0	39.0	41.0	35.0	41.0
23	38.66898498724739	41.0	39.0	41.0	35.0	41.0
24	38.63605956104268	41.0	39.0	41.0	35.0	41.0
25	38.57984200005137	41.0	39.0	41.0	35.0	41.0
26	38.54979092842768	41.0	39.0	41.0	35.0	41.0
27	38.49453491968898	41.0	39.0	41.0	35.0	41.0
28	38.4524171635872	41.0	39.0	41.0	34.0	41.0
29	38.39041878183234	40.0	39.0	41.0	34.0	41.0
30	38.34404320222561	40.0	39.0	41.0	34.0	41.0
31	38.292980817417714	40.0	39.0	41.0	34.0	41.0
32	38.24374439892977	40.0	38.0	41.0	34.0	41.0
33	38.18945922858009	40.0	38.0	41.0	34.0	41.0
34	38.13984044810877	40.0	38.0	41.0	34.0	41.0
35	38.08658684228192	40.0	38.0	41.0	34.0	41.0
36	38.039490535619386	40.0	38.0	41.0	33.0	41.0
37	37.98534373579094	40.0	38.0	41.0	33.0	41.0
38	37.94582945318149	40.0	38.0	41.0	33.0	41.0
39	37.89424865149586	40.0	38.0	41.0	33.0	41.0
40	37.82648806850796	40.0	38.0	41.0	33.0	41.0
41	37.75151763941781	40.0	38.0	41.0	33.0	41.0
42	37.670347828188895	40.0	38.0	41.0	33.0	41.0
43	37.62944870618246	40.0	38.0	41.0	32.0	41.0
44	37.56890106467562	40.0	38.0	41.0	32.0	41.0
45	37.51034623276922	40.0	38.0	41.0	32.0	41.0
46	37.449937883308614	40.0	38.0	41.0	32.0	41.0
47	37.38340316484289	40.0	37.0	41.0	32.0	41.0
48	37.6043840062675	40.0	38.0	41.0	33.0	41.0
49	37.6526005565113	40.0	38.0	41.0	33.0	41.0
50	37.62928042154239	40.0	38.0	41.0	33.0	41.0
51	36.12114098120153	38.0	36.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	187605.0
3	8404.0
4	4146.0
5	2190.0
6	4164.0
7	10702.0
8	28419.0
9	43343.0
10	43814.0
11	44675.0
12	50659.0
13	58439.0
14	65910.0
15	70563.0
16	72816.0
17	73597.0
18	74632.0
19	77347.0
20	79643.0
21	84049.0
22	90350.0
23	100367.0
24	113379.0
25	128481.0
26	147921.0
27	171607.0
28	202212.0
29	240907.0
30	287639.0
31	356543.0
32	455465.0
33	605460.0
34	857178.0
35	1309676.0
36	1971899.0
37	3178789.0
38	7216026.0
39	2.3788118E7
40	14019.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	21.89212582727291	30.359921390315524	26.98244593653254	20.765506845879024
2	23.71474507895662	31.477563494956655	26.942293092827775	17.86539833325895
3	22.04525390329427	29.53897993327082	29.027415185149685	19.388350978285228
4	21.544691293006075	26.31493252120437	30.65617991318336	21.484196272606194
5	23.243006169536745	26.320740632224997	31.218927875899606	19.21732532233865
6	20.845921249277556	25.301768632219108	33.980867800447214	19.87144231805612
7	23.149327858478408	25.63895257214121	32.38645262129067	18.825266948089713
8	24.28337775485925	26.05853161003887	30.299436145970944	19.358654489130934
9	22.560148246380564	26.87240037441373	31.36845323382831	19.198998145377395
10	24.00328035665108	27.11743062497635	29.734457175223543	19.144831843149028
11	24.0772126908977	25.71701492282185	30.80204899901012	19.403723387270333
12	21.741715400767006	27.77128464552396	30.9081243019263	19.578875651782738
13	22.859539068297234	26.196762904817582	31.135579989146656	19.808118037738524
14	24.70925134670785	26.544257156803663	28.77874636309789	19.967745133390597
15	23.02708000154149	28.78780464043052	28.002171952526005	20.182943405501984
16	23.979663788377	27.229638739250213	28.339901968838817	20.450795503533975
17	23.573569659165454	27.159670468131925	28.650147905461687	20.616611967240935
18	23.602452526535824	26.855931115131554	28.650635024782474	20.890981333550144
19	23.322237278820037	27.061063193175887	28.801974729455004	20.81472479854907
20	23.146263079149936	27.17679532984747	28.785893692950577	20.89104789805202
21	22.896372003873527	27.349803760962814	28.848696465208334	20.90512776995532
22	22.640658503603834	27.60856489166449	28.9930778036848	20.75769880104688
23	22.49052397286245	27.7046991323531	29.004823997332885	20.79995289745156
24	22.39605366565796	27.748750217939318	28.951758622705707	20.903437493697012
25	22.369369096712678	27.816018813455614	28.90773573987852	20.906876349953194
26	22.35871916060871	27.853668981853986	28.85803978220462	20.92957207533268
27	22.2664363128617	27.834442644384723	28.793434655278666	21.105686387474908
28	22.303305006677142	27.858150140794073	28.76161066009642	21.07693419243236
29	22.32929636420019	27.906465502844704	28.676111774761743	21.088126358193364
30	22.325993559220308	27.88840456810715	28.575787804569728	21.209814068102805
31	22.39087096528896	27.88580406348461	28.534146280799316	21.189178690427116
32	22.40924497450228	27.873331046698556	28.48488664703107	21.232537331768093
33	22.33258101942434	27.866846969916605	28.469476371990343	21.331095638668714
34	22.416811802377314	27.857606872654262	28.396395954686067	21.329185370282357
35	22.361606521629756	27.925464075056055	28.385725316710502	21.327204086603686
36	22.31906229902222	27.88865685841455	28.402798863868185	21.389481978695045
37	22.353459468659956	27.872854583526202	28.37718446480857	21.396501483005274
38	22.311977782719026	27.884364023774793	28.40986265683877	21.393795536667408
39	22.271897315860823	27.82773065499396	28.422904601737407	21.477467427407806
40	22.35095954102222	27.84353140762576	28.320966117375036	21.484542933976986
41	22.367175482683475	27.844475116848884	28.332119143623576	21.45623025684407
42	22.294344933432093	27.7966076474805	28.346568410750073	21.562479008337334
43	22.373771335026497	27.867097911484795	28.289423977590967	21.469706775897745
44	22.418118759474297	27.874738407312925	28.208908905357937	21.49823392785484
45	22.35342811915928	27.847917189518316	28.244099536475233	21.554555154847176
46	22.32331753432677	27.891354923015093	28.193121990587876	21.59220555207026
47	22.34524408417168	27.882486854440668	28.19508509367994	21.577183967707718
48	22.306371051128345	27.855433904628295	28.215537352234083	21.622657692009284
49	22.288929046355832	27.917288226702524	28.16430131709902	21.629481409842622
50	22.287270579491285	27.914355599614943	28.161389121184655	21.636984699709117
51	22.288421030882738	27.875757540134472	28.17159078886294	21.664230640119854
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	42.65763267315194
2	41.58014341221557
3	41.4336048815795
4	43.028887565612266
5	42.460331491875394
6	40.71736356733367
7	41.97459480656811
8	43.64203224399018
9	41.75914639175796
10	43.1481121998001
11	43.48093607816803
12	41.320591052549744
13	42.667657106035755
14	44.67699648009844
15	43.21002340704347
16	44.43045929191097
17	44.190181626406385
18	44.493433860085965
19	44.136962077369105
20	44.037310977201955
21	43.80149977382885
22	43.398357304650716
23	43.290476870314016
24	43.29949115935497
25	43.276245446665875
26	43.28829123594139
27	43.37212270033661
28	43.3802391991095
29	43.41742272239355
30	43.535807627323116
31	43.580049655716074
32	43.64178230627037
33	43.66367665809305
34	43.74599717265967
35	43.68881060823344
36	43.708544277717266
37	43.74996095166523
38	43.70577331938643
39	43.74936474326863
40	43.8355024749992
41	43.823405739527544
42	43.85682394176943
43	43.84347811092424
44	43.91635268732914
45	43.907983274006455
46	43.91552308639703
47	43.92242805187939
48	43.92902874313763
49	43.91841045619845
50	43.9242552792004
51	43.95265167100259
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	260187.0
1	142648.5
2	25110.0
3	27180.0
4	29250.0
5	65060.0
6	100870.0
7	111489.0
8	122108.0
9	93484.0
10	64860.0
11	62642.5
12	60425.0
13	68299.5
14	76174.0
15	100114.0
16	124054.0
17	166742.5
18	209431.0
19	261035.5
20	312640.0
21	371599.0
22	430558.0
23	513514.0
24	596470.0
25	704772.5
26	956769.0
27	1100463.0
28	1214320.0
29	1328177.0
30	1432507.0
31	1536837.0
32	1670273.0
33	1803709.0
34	1920472.5
35	2037236.0
36	2213870.5
37	2390505.0
38	2506602.5
39	2622700.0
40	2734690.0
41	2846680.0
42	2890394.5
43	2934109.0
44	2954733.5
45	2975358.0
46	2919558.5
47	2863759.0
48	2787478.5
49	2711198.0
50	2593793.0
51	2476388.0
52	2312424.5
53	2148461.0
54	1980832.5
55	1813204.0
56	1640182.5
57	1467161.0
58	1308921.5
59	1150682.0
60	1005400.5
61	860119.0
62	734945.5
63	609772.0
64	517013.0
65	424254.0
66	355748.0
67	287242.0
68	244781.0
69	202320.0
70	172866.0
71	143412.0
72	121086.0
73	98760.0
74	83662.0
75	57597.0
76	46630.0
77	41643.0
78	36656.0
79	34197.5
80	31739.0
81	27712.0
82	23685.0
83	20949.5
84	18214.0
85	15590.5
86	12967.0
87	12896.0
88	12825.0
89	9394.0
90	5963.0
91	4396.0
92	2829.0
93	2276.5
94	1724.0
95	1347.0
96	970.0
97	725.5
98	481.0
99	334.5
100	188.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.880486408297997
2	0.523123743816715
3	0.5090527661191083
4	0.5046058173320562
5	0.4982378433782274
6	0.49385705535952673
7	0.48374154645550416
8	0.4381378739846714
9	0.45199146630055187
10	0.455580687983619
11	0.4728084794854242
12	0.46384133248921644
13	0.4497892578682816
14	0.4412356156742705
15	0.4868770942984469
16	0.4582483846789335
17	0.48134085571817953
18	0.490348171752315
19	0.5006739773843117
20	0.5240878007269792
21	0.5100782580285561
22	0.5425159375974469
23	0.5133933850998813
24	0.538770765531837
25	0.5344254207818959
26	0.5202315730859223
27	0.5214839019154321
28	0.514681157198151
29	0.5333290423349288
30	0.5199267609745888
31	0.5342482044380975
32	0.5576832937420206
33	0.5186295373379832
34	0.5301037048777948
35	0.5297374577672778
36	0.5399238532088197
37	0.5337472729062934
38	0.5459066769754596
39	0.5246359899504629
40	0.5349381667366199
41	0.5531512811099452
42	0.5927981215445619
43	0.5171172912042354
44	0.5535151653358782
45	0.5582054912350805
46	0.5397348224421012
47	0.5284993062452717
48	0.5340757138634668
49	0.5517453647824766
50	0.530318727374937
51	0.5048255655983663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.2321153E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	33.4081762741535
#Duplication Level	Relative count
1	100.0
2	18.015829928279565
3	6.121953310283739
4	3.086693145126725
5	1.8973054833271423
6	1.21724719261651
7	0.9479383947195474
8	0.7357773523444867
9	0.5921936165957082
10++	10.254450381461268
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	175489	0.4146602527582365	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	22857510	5.6388383	6.9137425	17
TGTGT	9739950	3.9403353	23.372046	7
AAAAA	11803510	3.7146199	4.095002	44
GGGGG	3722410	3.162454	6.3249173	16
GTTTG	7587760	3.0696583	22.97377	10
TGTTT	9252720	2.923062	18.441471	9
GTGTT	6944425	2.8093946	22.742191	8
TTTGA	7989730	2.6500201	18.984821	11
CTTTG	5905575	2.6072896	24.337025	4
TTGTG	5979710	2.4191153	22.596882	6
GAGGG	3364925	2.343776	8.5949	14
TTTGT	6841060	2.161185	17.622334	5
TTGAG	5078455	2.1570318	14.895587	12
TGAGG	3814425	2.0747313	9.838152	13
AGGGG	2663790	1.8554133	5.401248	15
ACTTT	5072465	1.8360596	20.018448	3
AACTT	4790125	1.8203843	21.52238	2
TAACT	3631045	1.3799008	21.23985	1
GTAAC	1731770	0.8427801	8.514735	1
>>END_MODULE