FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_4_CCGTCC_L006_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_4_CCGTCC_L006_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 42321153
Filtered Sequences 0
Sequence length 51
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63627 0.1503432574249572 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 19616710 5.163861 6.147469 18
AAAAA 11737130 3.5186288 3.8809245 47
TGTGT 7473355 3.2108994 21.333567 4
TGTTT 7282405 2.4490886 16.647388 6
GTTTG 5631100 2.4193814 20.593033 7
CTTTG 4682645 2.126087 21.487005 1
GTGTT 4946110 2.1250782 20.453514 5
TTTGA 6068420 2.094585 16.723885 8
GAGGG 2882560 2.0746505 6.8794765 11
TGAGG 3315040 1.8675572 8.308557 10
TTTGT 5544290 1.864557 16.101866 2
TTGTG 4130795 1.7747811 20.461311 3
TTGAG 3925640 1.7310685 12.9222145 9
GTAAC 1525695 0.7296973 5.070604 2
Produced by FastQC (version 0.10.1)