##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_4_CCGTCC_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	42321153	
Filtered Sequences	0	
Sequence length	51	
%GC	43	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.8779108877303	34.0	31.0	34.0	31.0	34.0
2	33.09708083803861	34.0	33.0	34.0	31.0	34.0
3	33.12400156016543	34.0	33.0	34.0	31.0	34.0
4	36.48173791484367	37.0	37.0	37.0	35.0	37.0
5	36.41601843881711	37.0	37.0	37.0	35.0	37.0
6	36.399584292989374	37.0	37.0	37.0	35.0	37.0
7	36.40279358646018	37.0	37.0	37.0	35.0	37.0
8	36.38688993185039	37.0	37.0	37.0	35.0	37.0
9	38.19297739832372	39.0	39.0	39.0	37.0	39.0
10	38.1668958073992	39.0	39.0	39.0	37.0	39.0
11	38.135498056964565	39.0	39.0	39.0	37.0	39.0
12	38.102725036815514	39.0	38.0	39.0	37.0	39.0
13	38.09562215849837	39.0	38.0	39.0	37.0	39.0
14	39.644458812357975	41.0	40.0	41.0	37.0	41.0
15	39.62354546436861	41.0	40.0	41.0	37.0	41.0
16	39.60393040331392	41.0	40.0	41.0	37.0	41.0
17	39.57540348676228	41.0	40.0	41.0	37.0	41.0
18	39.53630849802225	41.0	40.0	41.0	37.0	41.0
19	39.535785827952274	41.0	40.0	41.0	37.0	41.0
20	39.51013707022585	41.0	40.0	41.0	37.0	41.0
21	39.44012685571208	41.0	39.0	41.0	37.0	41.0
22	39.40067093635185	41.0	39.0	41.0	36.0	41.0
23	39.35223908006476	41.0	39.0	41.0	36.0	41.0
24	39.33778656739338	41.0	39.0	41.0	36.0	41.0
25	39.272189394272885	41.0	39.0	41.0	36.0	41.0
26	39.187073447644494	41.0	39.0	41.0	36.0	41.0
27	39.13290724853361	40.0	39.0	41.0	36.0	41.0
28	39.07467067355183	40.0	39.0	41.0	36.0	41.0
29	39.015888744808066	40.0	39.0	41.0	36.0	41.0
30	38.94770088140084	40.0	39.0	41.0	35.0	41.0
31	38.86999680750664	40.0	39.0	41.0	35.0	41.0
32	38.80465354996354	40.0	38.0	41.0	35.0	41.0
33	38.734171160223354	40.0	38.0	41.0	35.0	41.0
34	38.666300915761916	40.0	38.0	41.0	35.0	41.0
35	38.59973866969078	40.0	38.0	41.0	35.0	41.0
36	38.520056223420944	40.0	38.0	41.0	35.0	41.0
37	38.719677722390976	40.0	38.0	41.0	35.0	41.0
38	38.754896115424835	40.0	38.0	41.0	35.0	41.0
39	38.737448315739414	40.0	38.0	41.0	35.0	41.0
40	38.70548238607771	40.0	38.0	41.0	35.0	41.0
41	38.6500625585508	40.0	38.0	41.0	35.0	41.0
42	38.59108666061154	40.0	38.0	41.0	35.0	41.0
43	38.55235574512821	40.0	38.0	41.0	35.0	41.0
44	38.499279284758614	40.0	38.0	41.0	35.0	41.0
45	38.433699786014806	40.0	38.0	41.0	34.0	41.0
46	38.3809934242576	40.0	38.0	41.0	34.0	41.0
47	38.30277313096833	40.0	38.0	41.0	34.0	41.0
48	38.24900637749638	40.0	38.0	41.0	34.0	41.0
49	38.193763600911346	40.0	38.0	41.0	34.0	41.0
50	38.2816472178818	40.0	38.0	41.0	34.0	41.0
51	38.020891940255034	40.0	38.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	13.0
10	50.0
11	96.0
12	216.0
13	385.0
14	667.0
15	1138.0
16	1688.0
17	2597.0
18	4155.0
19	6791.0
20	11405.0
21	18531.0
22	29295.0
23	43027.0
24	60466.0
25	81825.0
26	108014.0
27	141480.0
28	182586.0
29	234467.0
30	297410.0
31	378393.0
32	485102.0
33	637326.0
34	890785.0
35	1358067.0
36	2003099.0
37	3297188.0
38	7655937.0
39	2.4368868E7
40	20082.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.07387136627943	31.531720128314873	23.948018161427132	21.446390343978564
2	24.309023122349334	29.291964113565218	27.22505648270337	19.173956281382086
3	21.25807348061503	26.993384697914784	31.39720235671451	20.35133946475567
4	21.797676447992803	27.034334343395606	31.45122251277039	19.716766695841205
5	23.232392209481887	27.625974338734128	29.698827340162087	19.442806111621902
6	21.99441730710881	26.552667409604837	30.65906781887535	20.793847464411
7	23.90682866080685	27.140826929283758	29.125889913855556	19.826454496053834
8	22.608653880483836	27.362026266155837	29.568235062026783	20.461084791333544
9	21.468356497754208	27.85184751464593	30.3926525820315	20.28714340556837
10	22.368265817332528	27.16483173319971	30.265260022570743	20.201642426897017
11	23.82297334857583	26.88590974825284	29.00532034181583	20.2857965613555
12	22.146512859163767	28.96506272173493	28.369341228899625	20.519083190201677
13	22.91204826106699	27.463207346926488	28.982804414615078	20.641939977391445
14	22.55361689017134	27.445675423989474	29.149719126697242	20.850988559141946
15	22.673125186357755	27.49054828444773	28.893440119648915	20.9428864095456
16	22.881434728396933	27.522270009987675	28.535493822675388	21.060801438940004
17	22.70424910209795	27.80234744549611	28.448057641529758	21.04534581087618
18	22.61086554045444	27.45962757678176	28.691103949838038	21.238402932925766
19	22.439342381430613	27.64999540733511	28.71911864125932	21.19154356997495
20	22.293577398517474	27.688449319894477	28.829082232235027	21.188891049353025
21	22.341631382301898	27.74162367457238	28.660669996396365	21.25607494672936
22	22.28093832887776	27.864746501589877	28.63463100828089	21.219684161251468
23	22.239119997510464	27.937426468508548	28.603051055815985	21.220402478164996
24	22.106626741472766	27.86989445252984	28.62337119549241	21.40010761050498
25	22.155010757859078	27.943981110344513	28.636311019220106	21.264697112576307
26	22.162354688792117	27.908528078006817	28.52375278041829	21.40536445278277
27	22.121776786185386	27.84900732737598	28.468387900490328	21.560827985948304
28	22.16307764582879	27.97680866586976	28.453912869528864	21.40620081877259
29	22.21324017375621	27.9739292439795	28.41758010917478	21.395250473089515
30	22.121393175988068	27.926398668765795	28.448760743713503	21.50344741153263
31	22.219659280076797	27.936389162176184	28.283572519869676	21.560379037877347
32	22.202878073761365	28.04291981364496	28.26446387223902	21.489738240354654
33	22.178330037816103	27.91556632968603	28.224575121181793	21.681528511316074
34	22.215675456668205	27.92958169168973	28.19709803274972	21.65764481889234
35	22.196835107313678	27.947524260877888	28.19414817457922	21.661492457229215
36	22.2752971743511	27.91148658759629	28.177846940408564	21.63536929764405
37	22.249626474631278	27.935588517951143	28.213794976159402	21.600990031258174
38	22.199132901695755	27.946299100121397	28.17190259443073	21.682665403752114
39	22.170659569111628	27.86984689256931	28.19364338254806	21.765850155771
40	22.17559905029941	28.020397149367014	28.13184710394227	21.6721566963913
41	22.196678648619802	27.908095213014906	28.241614056443225	21.653612081922073
42	22.143055270926133	27.873230927231564	28.13880524072253	21.84490856111977
43	22.214985486674877	27.947600048656575	28.159371441787396	21.67804302288116
44	22.199684566282894	27.942809273090614	28.064605241061617	21.792900919564875
45	22.263776510224446	27.891350685617006	28.100038663949878	21.74483414020867
46	22.183671642727738	27.94110558383465	28.140484029876028	21.734738743561593
47	22.20381807375969	28.023267162367976	28.059051708349926	21.713863055522406
48	22.189092962826887	27.895346006964516	28.13664645145439	21.778914578754204
49	22.28374268722289	27.950879848984094	28.035727758981366	21.729649704811646
50	22.177663012193936	27.932750461721017	28.054003843860993	21.835582682224057
51	22.17794727851578	27.914759316694422	28.026734054244695	21.880559350545102
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.520261710258
2	43.48297940373142
3	41.60941294537071
4	41.51444314383401
5	42.675198321103785
6	42.78826477151981
7	43.733283156860686
8	43.069738671817376
9	41.75549990332258
10	42.56990824422955
11	44.108769909931326
12	42.66559604936545
13	43.55398823845844
14	43.40460544931328
15	43.61601159590335
16	43.94223616733694
17	43.74959491297413
18	43.84926847338021
19	43.63088595140556
20	43.4824684478705
21	43.597706329031254
22	43.500622490129224
23	43.45952247567546
24	43.50673435197775
25	43.41970787043538
26	43.56771914157489
27	43.682604772133686
28	43.56927846460138
29	43.60849064684572
30	43.6248405875207
31	43.780038317954144
32	43.692616314116016
33	43.85985854913218
34	43.873320275560545
35	43.85832756454289
36	43.91066647199515
37	43.85061650588945
38	43.881798305447866
39	43.936509724882626
40	43.84775574669071
41	43.85029073054187
42	43.9879638320459
43	43.89302850955604
44	43.99258548584777
45	44.00861065043312
46	43.91841038628933
47	43.9176811292821
48	43.968007541581095
49	44.01339239203454
50	44.01324569441799
51	44.058506629060886
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	113104.0
1	66150.0
2	19196.0
3	22317.5
4	25439.0
5	44972.0
6	64505.0
7	72659.5
8	80814.0
9	62711.0
10	44608.0
11	48246.5
12	51885.0
13	60753.0
14	69621.0
15	96061.0
16	122501.0
17	166405.5
18	210310.0
19	263478.5
20	316647.0
21	374690.5
22	432734.0
23	515417.0
24	598100.0
25	708365.5
26	966377.5
27	1114124.0
28	1221953.0
29	1329782.0
30	1426345.5
31	1522909.0
32	1652596.0
33	1782283.0
34	1895877.0
35	2009471.0
36	2184239.5
37	2359008.0
38	2471602.0
39	2584196.0
40	2699969.5
41	2815743.0
42	2861154.5
43	2906566.0
44	2937890.0
45	2969214.0
46	2920072.0
47	2870930.0
48	2807726.0
49	2744522.0
50	2636231.5
51	2527941.0
52	2369053.0
53	2210165.0
54	2044332.5
55	1878500.0
56	1720638.5
57	1562777.0
58	1380885.5
59	1198994.0
60	1047684.0
61	896374.0
62	766945.5
63	637517.0
64	539180.0
65	440843.0
66	368746.0
67	296649.0
68	252738.0
69	208827.0
70	177601.0
71	146375.0
72	122511.0
73	98647.0
74	82415.5
75	54879.5
76	43575.0
77	38621.5
78	33668.0
79	31957.5
80	30247.0
81	26162.5
82	22078.0
83	19401.5
84	16725.0
85	14098.5
86	11472.0
87	11401.5
88	11331.0
89	7577.0
90	3823.0
91	2427.0
92	1031.0
93	726.5
94	422.0
95	263.5
96	105.0
97	66.5
98	28.0
99	20.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.26652392953471754
2	0.009961921406063771
3	7.561230668739105E-5
4	0.0
5	3.3080384175733586E-5
6	0.0
7	0.002802381116601431
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0068240106785370435
13	0.0
14	2.6936884257383067E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.008681237961546085
20	0.0
21	0.0
22	0.0
23	0.0
24	1.4177307503885824E-5
25	0.0
26	0.004680874360866303
27	0.0
28	0.0
29	0.0022163857397741503
30	2.953605729976213E-4
31	0.0
32	0.0
33	6.379788376748621E-5
34	0.0
35	8.0338075855353E-5
36	6.852365293544815E-4
37	1.5358749795876307E-4
38	0.0
39	0.00467614859169834
40	0.00404053263860746
41	1.4177307503885824E-5
42	9.924115252720077E-5
43	1.0632980627914366E-4
44	8.884446035768449E-4
45	1.7957922838255375E-4
46	3.048121113335452E-4
47	0.009458626989675823
48	1.2759576753497242E-4
49	0.004494206478731806
50	0.0016162130554429838
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4.2321153E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	35.154160413934804
#Duplication Level	Relative count
1	100.0
2	18.997525204225404
3	6.5549236755462434
4	3.3427993663354774
5	2.048459275373957
6	1.4235550915820443
7	1.0081690161409247
8	0.8395324899073594
9	0.6307444098086596
10++	11.159941889751133
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63627	0.1503432574249572	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	19616710	5.163861	6.147469	18
AAAAA	11737130	3.5186288	3.8809245	47
TGTGT	7473355	3.2108994	21.333567	4
TGTTT	7282405	2.4490886	16.647388	6
GTTTG	5631100	2.4193814	20.593033	7
CTTTG	4682645	2.126087	21.487005	1
GTGTT	4946110	2.1250782	20.453514	5
TTTGA	6068420	2.094585	16.723885	8
GAGGG	2882560	2.0746505	6.8794765	11
TGAGG	3315040	1.8675572	8.308557	10
TTTGT	5544290	1.864557	16.101866	2
TTGTG	4130795	1.7747811	20.461311	3
TTGAG	3925640	1.7310685	12.9222145	9
GTAAC	1525695	0.7296973	5.070604	2
>>END_MODULE