FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_2_GATCAG_L006_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_2_GATCAG_L006_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34096341
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 142912 0.4191417489636205 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 8600355 4.308103 41.310043 4
TTTTT 12504950 4.162648 5.3621373 17
AAAAA 7524890 3.4427872 3.55675 43
GTTTG 6269125 3.1403399 40.717903 7
TGTTT 7649575 3.1236768 33.739418 6
GTGTT 5927300 2.9691122 40.670216 5
TTTGA 6767520 2.9449825 35.582283 8
CTTTG 5130455 2.780785 44.17829 1
TTGTG 5460475 2.7352695 40.696434 3
GGGGG 2734265 2.5283265 5.33458 13
TTTGT 5857275 2.391797 33.434593 2
TTGAG 4230800 2.258482 25.628786 9
GAGGG 2780080 2.2332294 11.143549 11
TGAGG 3076545 2.0146444 14.718448 10
ACTTT 3492210 1.6443539 15.515175 3
AGGGG 2044150 1.6420591 6.2777743 12
AACTT 3171195 1.5912645 17.049292 2
TGAGC 2094900 1.4843634 5.724689 10
TGAGT 2457130 1.311663 7.505667 10
GAGTG 1971110 1.2907612 5.7695446 11
TAACT 2467090 1.2379537 16.891928 1
TTGAT 2743610 1.1939209 7.9215827 9
TTGAC 1796840 1.0378757 7.370726 9
GTAAC 1193715 0.7347864 5.318273 1
Produced by FastQC (version 0.10.1)