##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_2_GATCAG_L006_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34096341	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24994687846417	34.0	31.0	34.0	31.0	34.0
2	32.42869960738602	34.0	31.0	34.0	31.0	34.0
3	32.50585665482404	34.0	31.0	34.0	31.0	34.0
4	35.87159343578831	37.0	37.0	37.0	35.0	37.0
5	35.89027505913318	37.0	37.0	37.0	35.0	37.0
6	35.864514758343134	37.0	37.0	37.0	35.0	37.0
7	35.89854808760858	37.0	37.0	37.0	35.0	37.0
8	35.90234057666188	37.0	37.0	37.0	35.0	37.0
9	37.658543126372415	39.0	38.0	39.0	35.0	39.0
10	37.645135851967225	39.0	38.0	39.0	35.0	39.0
11	37.645346050475034	39.0	38.0	39.0	35.0	39.0
12	37.60248156833016	39.0	38.0	39.0	35.0	39.0
13	37.602651527916144	39.0	38.0	39.0	35.0	39.0
14	39.09258078454811	41.0	40.0	41.0	36.0	41.0
15	39.0505248642369	41.0	39.0	41.0	36.0	41.0
16	39.02893811978241	41.0	39.0	41.0	36.0	41.0
17	38.98636176239556	41.0	39.0	41.0	36.0	41.0
18	38.95421831333749	41.0	39.0	41.0	36.0	41.0
19	38.96116433725249	41.0	39.0	41.0	36.0	41.0
20	38.92219581567418	41.0	39.0	41.0	36.0	41.0
21	38.88852143988119	41.0	39.0	41.0	36.0	41.0
22	38.845703472991424	41.0	39.0	41.0	36.0	41.0
23	38.80993168739133	41.0	39.0	41.0	35.0	41.0
24	38.77471567990243	41.0	39.0	41.0	35.0	41.0
25	38.72100909596135	41.0	39.0	41.0	35.0	41.0
26	38.69069071077158	41.0	39.0	41.0	35.0	41.0
27	38.63752283566146	41.0	39.0	41.0	35.0	41.0
28	38.59645919191153	41.0	39.0	41.0	35.0	41.0
29	38.541150559234495	41.0	39.0	41.0	35.0	41.0
30	38.49295436129056	40.0	39.0	41.0	35.0	41.0
31	38.44146745834106	40.0	39.0	41.0	34.0	41.0
32	38.38730633882386	40.0	38.0	41.0	34.0	41.0
33	38.32963783415939	40.0	38.0	41.0	34.0	41.0
34	38.28000500112314	40.0	38.0	41.0	34.0	41.0
35	38.22304076557658	40.0	38.0	41.0	34.0	41.0
36	38.168239958651284	40.0	38.0	41.0	34.0	41.0
37	38.11009072791711	40.0	38.0	41.0	34.0	41.0
38	38.06097020791762	40.0	38.0	41.0	33.0	41.0
39	38.0065497937154	40.0	38.0	41.0	33.0	41.0
40	37.92988576105571	40.0	38.0	41.0	33.0	41.0
41	37.848672970510236	40.0	38.0	41.0	33.0	41.0
42	37.75886741043563	40.0	38.0	41.0	33.0	41.0
43	37.71067036196054	40.0	38.0	41.0	33.0	41.0
44	37.63916735229742	40.0	38.0	41.0	33.0	41.0
45	37.574707503071956	40.0	37.0	41.0	32.0	41.0
46	37.50515696097713	40.0	37.0	41.0	32.0	41.0
47	37.43208888601859	40.0	37.0	41.0	32.0	41.0
48	37.65075836143239	40.0	37.0	41.0	33.0	41.0
49	37.69578814923279	40.0	37.0	41.0	33.0	41.0
50	37.66734800077228	40.0	37.0	41.0	33.0	41.0
51	36.1496036187578	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152822.0
3	6705.0
4	3366.0
5	1754.0
6	3001.0
7	5236.0
8	10387.0
9	15411.0
10	16983.0
11	20442.0
12	27005.0
13	34187.0
14	41001.0
15	45355.0
16	47923.0
17	49373.0
18	51107.0
19	52580.0
20	56506.0
21	60657.0
22	65687.0
23	74123.0
24	84411.0
25	97347.0
26	112780.0
27	132816.0
28	157392.0
29	190146.0
30	233840.0
31	292657.0
32	377973.0
33	506734.0
34	731987.0
35	1106404.0
36	1684606.0
37	2727708.0
38	5964961.0
39	1.8842151E7
40	10817.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.776666789668475	25.718689852596388	30.303290797492927	24.201352560242206
2	21.738234354247332	27.87644691335471	32.014925896819	18.37039283557896
3	22.565145588074927	26.930422016991972	31.09317844928514	19.411253945647957
4	21.50588608049812	24.5895243809709	32.51866312814185	21.38592641038913
5	27.52809690920734	24.554851008000973	28.562612743428232	19.354439339363452
6	21.31929978580229	24.196188230471183	34.637033345356514	19.847478638370013
7	25.887434287172933	24.723858688859025	30.086391429039374	19.30231559492867
8	22.9067448484072	24.849379945860356	32.62923240857074	19.614642797161693
9	22.86376264416002	25.069349693725833	32.41755193452433	19.64933572758981
10	22.85117751927391	26.621311713152757	30.902041232193778	19.625469535379555
11	26.9160168384848	24.84215504479841	28.299542248230118	19.942285868486668
12	21.735674251464257	29.704276107816867	28.493087238766247	20.066962401952626
13	24.58636381434566	25.34118571635779	29.323491072878298	20.74895939641825
14	25.349235179802402	25.82288440734028	27.875413493356977	20.952466919500342
15	24.199416430547142	27.51056503102206	27.168822213389255	21.121196325041545
16	24.651237084176813	26.29231452959963	27.607860178553956	21.4485882076696
17	24.310575657971327	26.31971365495299	27.875491445999927	21.49421924107575
18	24.076039925205084	26.30938261183278	27.9945112274902	21.620066235471935
19	23.69650171340138	26.643563874866427	28.29672721913114	21.363207192601052
20	23.296319511926235	26.71369507460727	28.402759101885156	21.58722631158134
21	23.380358157202995	26.82247774238393	28.197807321608593	21.59935677880448
22	23.07119777854067	26.994111070784083	28.465749135160195	21.46894201551505
23	23.070703588897906	27.1358269556235	28.25031770868374	21.543151746794862
24	22.86144125133757	27.20939850260413	28.22882508573049	21.700335160327803
25	22.888706337359118	27.389871504050234	28.0711606544089	21.65026150418175
26	22.88533459665537	27.305714556796808	28.050006654494574	21.758944192053253
27	22.89685074398384	27.328347573475526	27.94419549191764	21.830606190622994
28	22.851618066980308	27.427793301450524	27.897205461772444	21.82338316979672
29	22.95310440225942	27.362213855201862	27.783050045787295	21.901631696751426
30	22.96573846603226	27.32829392926069	27.767070052308235	21.938897552398824
31	23.069688709689643	27.274204364380132	27.64816510005102	22.007941825879207
32	23.098833162260053	27.34801613987895	27.566925046307766	21.986225651553227
33	23.05370543803106	27.25817029932765	27.55045586329069	22.137668399350602
34	23.069303891499807	27.305319667811318	27.550025466352114	22.075350974336764
35	23.16455396857149	27.243865481476732	27.44583717052808	22.145743379423706
36	23.119619826772816	27.246815485963428	27.492588058232712	22.14097662903104
37	23.07872275351117	27.2220403138988	27.50236523414255	22.196871698447477
38	23.054935835367086	27.27900526416124	27.58675473142208	22.079304169049603
39	23.03136482420737	27.13225375119132	27.56041037578506	22.275971048816253
40	23.05174737090301	27.191591890792786	27.576740338756185	22.17992039954802
41	23.070886429573196	27.200342077893154	27.56447819082369	22.164293301709964
42	22.978098814511945	27.181505862291903	27.534280793762182	22.30611452943397
43	23.059441128479502	27.259032237217312	27.43206982568345	22.24945680861974
44	23.104079663349534	27.14305884042086	27.4904197976014	22.2624416986282
45	23.088571304268957	27.157003817455287	27.387266349540866	22.367158528734887
46	22.98170332155902	27.285138475457664	27.336893150629994	22.39626505235332
47	23.04181892643312	27.20989713717484	27.39864327234038	22.349640664051666
48	22.97493318049801	27.095842899425133	27.337563492865524	22.591660427211334
49	23.05199642914824	27.20356285309026	27.325946636128577	22.418494081632918
50	22.93520879400845	27.20444524518409	27.381937181188736	22.478408779618725
51	22.99887206920617	27.172006704499406	27.325118401485465	22.504002824808957
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	43.97801934991069
2	40.108627189826294
3	41.97639953372288
4	42.89181249088724
5	46.882536248570794
6	41.16677842417231
7	45.1897498821016
8	42.5213876455689
9	42.51309837174983
10	42.47664705465347
11	46.85830270697147
12	41.80263665341688
13	45.33532321076391
14	46.301702099302744
15	45.32061275558869
16	46.099825291846415
17	45.80479489904708
18	45.69610616067702
19	45.059708906002435
20	44.883545823507575
21	44.979714936007476
22	44.54013979405572
23	44.61385533569276
24	44.56177641166538
25	44.53896784154087
26	44.644278788708625
27	44.727456934606835
28	44.67500123677702
29	44.85473609901084
30	44.904636018431084
31	45.07763053556885
32	45.08505881381328
33	45.19137383738166
34	45.14465486583657
35	45.310297347995196
36	45.26059645580386
37	45.275594451958646
38	45.13424000441669
39	45.307335873023625
40	45.231667770451025
41	45.23517973128317
42	45.284213343945915
43	45.30889793709924
44	45.36652136197773
45	45.45572983300384
46	45.37796837391234
47	45.391459590484786
48	45.56659360770934
49	45.47049051078116
50	45.41361757362717
51	45.50287489401513
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	168191.0
1	90049.0
2	11907.0
3	12960.0
4	14013.0
5	30751.5
6	47490.0
7	58939.0
8	70388.0
9	53537.5
10	36687.0
11	36135.5
12	35584.0
13	43045.5
14	50507.0
15	66321.5
16	82136.0
17	111412.0
18	140688.0
19	177339.0
20	213990.0
21	259572.0
22	305154.0
23	365445.0
24	425736.0
25	501837.5
26	674616.5
27	771294.0
28	857649.0
29	944004.0
30	1032888.5
31	1121773.0
32	1226334.0
33	1330895.0
34	1431213.5
35	1531532.0
36	1672311.0
37	1813090.0
38	1921610.0
39	2030130.0
40	2138836.0
41	2247542.0
42	2296310.0
43	2345078.0
44	2376092.0
45	2407106.0
46	2390276.5
47	2373447.0
48	2326925.5
49	2280404.0
50	2187726.0
51	2095048.0
52	1947996.5
53	1800945.0
54	1661234.5
55	1521524.0
56	1389346.5
57	1257169.0
58	1114992.5
59	972816.0
60	866759.5
61	760703.0
62	662322.5
63	563942.0
64	490919.5
65	417897.0
66	351778.5
67	285660.0
68	269552.0
69	253444.0
70	225054.5
71	196665.0
72	168724.0
73	140783.0
74	119144.5
75	82336.5
76	67167.0
77	62193.5
78	57220.0
79	58272.0
80	59324.0
81	52317.0
82	45310.0
83	41448.5
84	37587.0
85	33605.0
86	29623.0
87	33865.5
88	38108.0
89	25676.0
90	13244.0
91	8590.5
92	3937.0
93	3041.5
94	2146.0
95	1615.5
96	1085.0
97	849.0
98	613.0
99	391.5
100	170.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8880718315199863
2	0.5294497729243146
3	0.5154218747401664
4	0.5106325045259256
5	0.5043620369704772
6	0.49919725990539576
7	0.48891463163158766
8	0.44333202791466686
9	0.4565651194068009
10	0.46028106065691915
11	0.4777169491588555
12	0.46833471075386063
13	0.4552453296968141
14	0.4458630912918192
15	0.4924370037242412
16	0.4634749517550872
17	0.4879321215141531
18	0.49536400401438974
19	0.5061862796362812
20	0.5302973712047284
21	0.5156477054238753
22	0.5497950645202663
23	0.5188327979239767
24	0.5455218787259313
25	0.5409818021235768
26	0.5262001573717251
27	0.5279540112529962
28	0.5206863692500026
29	0.5399289032216096
30	0.5264494509836114
31	0.541090318166398
32	0.5652835299834666
33	0.5244726992846535
34	0.5366088988844874
35	0.535767166336118
36	0.5470850963157601
37	0.5404245575793603
38	0.5530358814747893
39	0.5313825316329397
40	0.5402984443404059
41	0.5590423910882403
42	0.5997036456199215
43	0.5228625558384696
44	0.5609516868686878
45	0.5656501382362407
46	0.5465718447618764
47	0.5353477664949444
48	0.5409964664536878
49	0.559312214762282
50	0.5374506314328567
51	0.5104565325645939
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4096341E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.841143286252663
#Duplication Level	Relative count
1	100.0
2	12.85141358281633
3	3.7085488890210305
4	1.770034290697905
5	1.0511849781636307
6	0.7750094150760144
7	0.5622617325061281
8	0.45985213375354306
9	0.3567818279122317
10++	10.606330895324176
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	142912	0.4191417489636205	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	8600355	4.308103	41.310043	4
TTTTT	12504950	4.162648	5.3621373	17
AAAAA	7524890	3.4427872	3.55675	43
GTTTG	6269125	3.1403399	40.717903	7
TGTTT	7649575	3.1236768	33.739418	6
GTGTT	5927300	2.9691122	40.670216	5
TTTGA	6767520	2.9449825	35.582283	8
CTTTG	5130455	2.780785	44.17829	1
TTGTG	5460475	2.7352695	40.696434	3
GGGGG	2734265	2.5283265	5.33458	13
TTTGT	5857275	2.391797	33.434593	2
TTGAG	4230800	2.258482	25.628786	9
GAGGG	2780080	2.2332294	11.143549	11
TGAGG	3076545	2.0146444	14.718448	10
ACTTT	3492210	1.6443539	15.515175	3
AGGGG	2044150	1.6420591	6.2777743	12
AACTT	3171195	1.5912645	17.049292	2
TGAGC	2094900	1.4843634	5.724689	10
TGAGT	2457130	1.311663	7.505667	10
GAGTG	1971110	1.2907612	5.7695446	11
TAACT	2467090	1.2379537	16.891928	1
TTGAT	2743610	1.1939209	7.9215827	9
TTGAC	1796840	1.0378757	7.370726	9
GTAAC	1193715	0.7347864	5.318273	1
>>END_MODULE