##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_2_GATCAG_L006_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34096341	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.93381844110487	34.0	33.0	34.0	31.0	34.0
2	33.150438195113075	34.0	34.0	34.0	31.0	34.0
3	33.18725742448434	34.0	34.0	34.0	31.0	34.0
4	36.52037610135351	37.0	37.0	37.0	35.0	37.0
5	36.44973215747696	37.0	37.0	37.0	35.0	37.0
6	36.39473259022134	37.0	37.0	37.0	35.0	37.0
7	36.417860819728425	37.0	37.0	37.0	35.0	37.0
8	36.39526511070499	37.0	37.0	37.0	35.0	37.0
9	38.218352549911444	39.0	39.0	39.0	37.0	39.0
10	38.19763369917024	39.0	39.0	39.0	37.0	39.0
11	38.16691283677624	39.0	39.0	39.0	37.0	39.0
12	38.12608663785947	39.0	38.0	39.0	37.0	39.0
13	38.12740965372208	39.0	38.0	39.0	37.0	39.0
14	39.67197600469798	41.0	40.0	41.0	37.0	41.0
15	39.649723206369856	41.0	40.0	41.0	37.0	41.0
16	39.62894044847803	41.0	40.0	41.0	37.0	41.0
17	39.60786977112881	41.0	40.0	41.0	37.0	41.0
18	39.568491968097106	41.0	40.0	41.0	37.0	41.0
19	39.56529819431358	41.0	40.0	41.0	37.0	41.0
20	39.53432129271584	41.0	40.0	41.0	37.0	41.0
21	39.47080743942583	41.0	39.0	41.0	37.0	41.0
22	39.430938058720145	41.0	39.0	41.0	37.0	41.0
23	39.39142367211778	41.0	39.0	41.0	36.0	41.0
24	39.37529361288357	41.0	39.0	41.0	36.0	41.0
25	39.30694718826281	41.0	39.0	41.0	36.0	41.0
26	39.221346712833494	41.0	39.0	41.0	36.0	41.0
27	39.167272142192616	40.0	39.0	41.0	36.0	41.0
28	39.10921954939388	40.0	39.0	41.0	36.0	41.0
29	39.053201603069375	40.0	39.0	41.0	36.0	41.0
30	38.983775678451835	40.0	39.0	41.0	35.0	41.0
31	38.90555690418511	40.0	38.0	41.0	35.0	41.0
32	38.83411126138139	40.0	38.0	41.0	35.0	41.0
33	38.7585518633803	40.0	38.0	41.0	35.0	41.0
34	38.688111841678264	40.0	38.0	41.0	35.0	41.0
35	38.61881012393676	40.0	38.0	41.0	35.0	41.0
36	38.5351089138861	40.0	38.0	41.0	35.0	41.0
37	38.73156151858054	40.0	38.0	41.0	35.0	41.0
38	38.75817029751081	40.0	38.0	41.0	35.0	41.0
39	38.73754447727983	40.0	38.0	41.0	35.0	41.0
40	38.69848670858847	40.0	38.0	41.0	35.0	41.0
41	38.64017661015298	40.0	38.0	41.0	35.0	41.0
42	38.57315900846956	40.0	38.0	41.0	35.0	41.0
43	38.52697592976325	40.0	38.0	41.0	35.0	41.0
44	38.46654882997563	40.0	38.0	41.0	34.0	41.0
45	38.39688296758881	40.0	38.0	41.0	34.0	41.0
46	38.337150986377104	40.0	38.0	41.0	34.0	41.0
47	38.25338545857457	40.0	38.0	41.0	34.0	41.0
48	38.19298287754689	40.0	38.0	41.0	34.0	41.0
49	38.13253310670491	40.0	38.0	41.0	34.0	41.0
50	38.217930539819505	40.0	38.0	41.0	34.0	41.0
51	37.953010940382136	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	6.0
10	10.0
11	28.0
12	53.0
13	111.0
14	210.0
15	343.0
16	632.0
17	1091.0
18	1855.0
19	3475.0
20	6390.0
21	10983.0
22	17840.0
23	27592.0
24	40408.0
25	55752.0
26	75950.0
27	101643.0
28	134023.0
29	173865.0
30	227193.0
31	293313.0
32	382128.0
33	513399.0
34	741966.0
35	1125791.0
36	1720912.0
37	2839108.0
38	6338116.0
39	1.9246412E7
40	15740.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.528408040236	26.683510872311683	28.002563567486533	24.785517519965783
2	22.309087976456755	26.527572346062016	31.788272709601728	19.375066967879498
3	21.156441685853736	25.543968607751307	33.062432113265025	20.237157593129933
4	21.98534147696376	25.367331937465075	32.38688573650762	20.260440849063542
5	26.577687393335292	25.74270896603828	27.899949935960848	19.779653704665577
6	22.162944111803668	25.382811604330215	31.80992353402378	20.644320749842336
7	26.495528659494155	26.05892389840168	27.280635305065488	20.164912137038673
8	21.996530360838427	25.99429363989526	31.43748474359756	20.571691255668753
9	21.317885693365163	26.19494273593756	31.882720201560634	20.604451369136648
10	21.833920537103968	25.97747365325798	31.61782960816822	20.57077620146983
11	26.34128688471294	25.849718009331262	27.164463189759864	20.644531916195934
12	21.771612593569888	30.627296846128566	26.851580543387737	20.74951001691381
13	24.292979120545517	26.378328982573233	27.968341236380763	21.36035066050049
14	23.572883528844653	26.64651018957048	28.28416602935292	21.496440252231945
15	23.788177153671707	26.68156093347377	27.89921944996972	21.6310424628848
16	23.66358020645089	26.66495797892214	27.866913930735265	21.804547883891708
17	23.541091989900032	26.800403597559047	27.800197094462426	21.8583073180785
18	23.318856999934393	26.778562544291777	28.019091550028786	21.883488905745047
19	22.98408979224108	27.10945308041208	28.29371546881516	21.612741658531675
20	22.679075740121206	27.143305494275765	28.398774519529823	21.77884424607321
21	22.847422249795073	27.156438868323146	28.139887502884843	21.856251378996944
22	22.732544820571803	27.2029247947749	28.269710817357208	21.79481956729609
23	22.86594329872522	27.24841354677911	28.042768577425946	21.842874577069722
24	22.615473991105674	27.31169413628491	28.093833894644867	21.978997977964553
25	22.71443437288476	27.48890269486688	27.900782667559547	21.895880264688813
26	22.700581516591374	27.384877659076167	27.86773652000804	22.046804304324414
27	22.72177240367229	27.406354247806235	27.775898299468555	22.095975049052917
28	22.71237256807116	27.46439273351941	27.790301604503544	22.032933093905882
29	22.793373761225862	27.452584647134255	27.703232809909036	22.05080878173085
30	22.767103289488038	27.35581639363956	27.761740822626585	22.11533949424582
31	22.891523756170788	27.33415001920587	27.57265654986264	22.2016696747607
32	22.93624116441116	27.408113967419556	27.511693996725338	22.14395087144395
33	22.839711564138234	27.31127982598706	27.515281343453097	22.333727266421604
34	22.837339056410773	27.3946403809136	27.542612270331297	22.22540829234433
35	22.92934948921458	27.36684968351711	27.450433498471416	22.253367328796887
36	22.972477790715068	27.295488340308715	27.478122131270144	22.253911737706073
37	22.95824983814214	27.27094675298108	27.455987494235075	22.314815914641702
38	22.885839861819775	27.373089681382528	27.55285677134681	22.18821368545088
39	22.89708317652756	27.211817827431204	27.51048864535563	22.380610350685608
40	22.87672708209378	27.28500368999101	27.568036940619976	22.270232287295233
41	22.90940226218435	27.22311783814197	27.55120879993649	22.316271099737193
42	22.799354264226743	27.310093098374104	27.489402882113396	22.401149755285758
43	22.89464613153469	27.343407564557083	27.402639799703778	22.35930650420446
44	22.945017643834195	27.176194354729745	27.484831306104198	22.393956695331866
45	22.973185843934125	27.141136008352344	27.378939069630498	22.506739078083033
46	22.829180406575684	27.316582021446063	27.405163649300277	22.44907392267797
47	22.85496226867519	27.35068243431024	27.375241709997866	22.4191135870167
48	22.83122786105675	27.184511379222894	27.382339243816684	22.601921515903673
49	22.98000833383268	27.218428334890316	27.36414575414044	22.437417577136568
50	22.825701947955636	27.21159150495881	27.378565164498138	22.58414138258742
51	22.869377684837207	27.232285716523073	27.320691097030032	22.577645501609688
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.31392556020178
2	41.68415494433626
3	41.393599278983665
4	42.245782326027296
5	46.35734109800087
6	42.807264861646004
7	46.66044079653283
8	42.56822161650718
9	41.92233706250181
10	42.4046967385738
11	46.98581880090887
12	42.521122610483694
13	45.65332978104601
14	45.0693237810766
15	45.41921961655651
16	45.468128090342596
17	45.399399307978534
18	45.202345905679444
19	44.596831450772754
20	44.45791998619441
21	44.70367362879202
22	44.5273643878679
23	44.70881787579494
24	44.59447196907023
25	44.61031463757357
26	44.74738582091579
27	44.81774745272521
28	44.74530566197704
29	44.84418254295671
30	44.88244278373386
31	45.093193430931485
32	45.08019203585511
33	45.173438830559846
34	45.0627473487551
35	45.18271681801147
36	45.22638952842114
37	45.27306575278385
38	45.07405354727066
39	45.27769352721317
40	45.14695936938901
41	45.22567336192155
42	45.2005040195125
43	45.25395263573915
44	45.33897433916606
45	45.479924922017155
46	45.278254329253656
47	45.27407585569189
48	45.43314937696042
49	45.41742591096924
50	45.40984333054306
51	45.4470231864469
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30397.0
1	18194.0
2	5991.0
3	8340.5
4	10690.0
5	19156.5
6	27623.0
7	43696.5
8	59770.0
9	45324.5
10	30879.0
11	31862.0
12	32845.0
13	41338.5
14	49832.0
15	67770.5
16	85709.0
17	117954.0
18	150199.0
19	190654.5
20	231110.0
21	279341.5
22	327573.0
23	390407.0
24	453241.0
25	534656.5
26	716453.0
27	816834.0
28	897261.5
29	977689.0
30	1060943.5
31	1144198.0
32	1243640.0
33	1343082.0
34	1436547.0
35	1530012.0
36	1662753.5
37	1795495.0
38	1896135.0
39	1996775.0
40	2098453.5
41	2200132.0
42	2246189.5
43	2292247.0
44	2321125.0
45	2350003.0
46	2337957.0
47	2325911.0
48	2291723.5
49	2257536.0
50	2171810.5
51	2086085.0
52	1956502.5
53	1826920.0
54	1685779.5
55	1544639.0
56	1415018.0
57	1285397.0
58	1143575.5
59	1001754.0
60	894716.5
61	787679.0
62	686716.0
63	585753.0
64	510786.0
65	435819.0
66	367192.5
67	298566.0
68	284540.5
69	270515.0
70	239664.5
71	208814.0
72	180023.5
73	151233.0
74	127508.0
75	87110.5
76	70438.0
77	65535.0
78	60632.0
79	62088.0
80	63544.0
81	55805.0
82	48066.0
83	43318.0
84	38570.0
85	34904.5
86	31239.0
87	36044.0
88	40849.0
89	26026.5
90	11204.0
91	6598.5
92	1993.0
93	1400.0
94	807.0
95	477.0
96	147.0
97	91.5
98	36.0
99	25.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2703075969354014
2	0.010276762541763644
3	7.625451657701335E-5
4	0.0
5	3.2261526244121035E-5
6	0.0
7	0.002853678639593615
8	0.0
9	0.0
10	0.0
11	0.0
12	0.006766121913198838
13	0.0
14	3.0795093233024624E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.008455452741981903
20	0.0
21	0.0
22	0.0
23	0.0
24	1.4664330110964106E-5
25	0.0
26	0.004982939371705603
27	0.0
28	0.0
29	0.002167387990400495
30	2.7275654006393235E-4
31	0.0
32	0.0
33	7.625451657701335E-5
34	0.0
35	7.918738259920617E-5
36	7.068207113484699E-4
37	1.935691574647262E-4
38	0.0
39	0.0047336457598192135
40	0.0041265424932252995
41	1.1731464088771285E-5
42	1.231803729320985E-4
43	1.231803729320985E-4
44	8.622626105246893E-4
45	1.7597196133156928E-4
46	3.1088379835243903E-4
47	0.009725383729591395
48	9.385171271017028E-5
49	0.004645659779153429
50	0.0015954791160728947
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4096341E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.709372035841564
#Duplication Level	Relative count
1	100.0
2	13.122325297041545
3	3.939289226761649
4	1.8347465511283592
5	1.1031073070891257
6	0.746258738470535
7	0.552882698492863
8	0.4718109566465538
9	0.3482097764546397
10++	10.78586427792988
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11653700	4.025397	5.0622425	17
TGTGT	6873880	3.6046014	43.394764	4
AAAAA	7476415	3.2549982	3.3859792	11
TGTTT	6240880	2.656109	35.246998	6
GTTTG	4826725	2.5310915	42.67747	7
CTTTG	4442540	2.453884	44.896473	1
TTTGA	5386160	2.4009426	36.518894	8
GTGTT	4476210	2.3472846	42.655556	5
TTTGT	5126650	2.1818943	34.91508	2
TTGTG	4059770	2.1289065	42.638702	3
GAGGG	2469820	2.0593655	11.340524	11
TTGAG	3414085	1.8751329	26.229464	9
TGAGG	2713075	1.8360088	15.312162	10
AGGGG	1814510	1.5129602	6.291974	12
TGAGC	2009600	1.4324845	5.743464	10
TGAGA	2476755	1.4247651	5.0239563	10
GAGTG	1837900	1.243755	5.8562407	11
TGAGT	2263285	1.243074	7.6622148	10
TTGAT	2473885	1.1027626	8.364473	9
TTGAC	1616785	0.935357	7.4183187	9
>>END_MODULE