FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_1_ACAGTG_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_1_ACAGTG_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35774422
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 167704 0.4687818576076505 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11497280 3.9553192 4.929223 16
TGTGT 6660240 3.4067857 29.06501 4
AAAAA 7900240 3.2729683 3.5041888 10
TGTTT 6261970 2.626828 23.970102 6
GTTTG 4809485 2.4601042 28.409313 7
TTTGA 5406300 2.3537693 24.443079 8
CTTTG 4302435 2.3125682 30.057276 1
GTGTT 4451360 2.2769194 28.386566 5
TTGTG 4179835 2.1380315 28.429565 3
TTTGT 4949860 2.0764124 23.646833 2
GAGGG 2547575 2.010914 7.5440116 11
TTGAG 3377595 1.7931042 17.0899 9
TGAGG 2740010 1.7737173 9.996357 10
ACTTT 3212975 1.4699289 9.020359 3
AACTT 2913860 1.3835685 9.6264925 2
TGAGT 2255320 1.1973087 5.1498013 10
TTGAT 2564205 1.1163914 5.8073077 9
TAACT 2178140 1.0342315 9.428775 1
TTGAC 1689640 0.94257826 5.3001685 9
Produced by FastQC (version 0.10.1)