##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_1_ACAGTG_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35774422	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42366948653985	34.0	31.0	34.0	31.0	34.0
2	32.54761659042318	34.0	31.0	34.0	31.0	34.0
3	32.62529745414196	34.0	31.0	34.0	31.0	34.0
4	35.98707470382051	37.0	37.0	37.0	35.0	37.0
5	35.997490749116785	37.0	37.0	37.0	35.0	37.0
6	36.01215083223427	37.0	37.0	37.0	35.0	37.0
7	36.011657602741984	37.0	37.0	37.0	35.0	37.0
8	36.005114100795254	37.0	37.0	37.0	35.0	37.0
9	37.78956059723341	39.0	39.0	39.0	37.0	39.0
10	37.75803857851288	39.0	39.0	39.0	37.0	39.0
11	37.76490298012362	39.0	39.0	39.0	37.0	39.0
12	37.71837920959282	39.0	39.0	39.0	35.0	39.0
13	37.71628078295716	39.0	39.0	39.0	35.0	39.0
14	39.25375887274992	41.0	40.0	41.0	37.0	41.0
15	39.214885763912555	41.0	40.0	41.0	37.0	41.0
16	39.1991133777088	41.0	40.0	41.0	37.0	41.0
17	39.181494420790365	41.0	40.0	41.0	37.0	41.0
18	39.15819864259442	41.0	40.0	41.0	36.0	41.0
19	39.15664473908202	41.0	40.0	41.0	37.0	41.0
20	39.123937655792176	41.0	40.0	41.0	36.0	41.0
21	39.08518541543452	41.0	40.0	41.0	36.0	41.0
22	39.037162389374174	41.0	40.0	41.0	36.0	41.0
23	39.013091001162785	41.0	39.0	41.0	36.0	41.0
24	38.98371928972046	41.0	39.0	41.0	36.0	41.0
25	38.91703782104432	41.0	39.0	41.0	36.0	41.0
26	38.88020988850637	41.0	39.0	41.0	36.0	41.0
27	38.830719026012495	41.0	39.0	41.0	35.0	41.0
28	38.79288302687322	41.0	39.0	41.0	35.0	41.0
29	38.742694179657185	41.0	39.0	41.0	35.0	41.0
30	38.70461733805231	41.0	39.0	41.0	35.0	41.0
31	38.6313935414526	41.0	39.0	41.0	35.0	41.0
32	38.58959286609858	41.0	39.0	41.0	35.0	41.0
33	38.51384969965413	40.0	39.0	41.0	35.0	41.0
34	38.49349185851277	40.0	39.0	41.0	35.0	41.0
35	38.42983148686511	40.0	38.0	41.0	35.0	41.0
36	38.37898728314884	40.0	38.0	41.0	34.0	41.0
37	38.32958053102857	40.0	38.0	41.0	34.0	41.0
38	38.27693221710193	40.0	38.0	41.0	34.0	41.0
39	38.21852154592463	40.0	38.0	41.0	34.0	41.0
40	38.1548484277398	40.0	38.0	41.0	34.0	41.0
41	38.0902718428267	40.0	38.0	41.0	33.0	41.0
42	38.013159933094094	40.0	38.0	41.0	33.0	41.0
43	37.9558233812974	40.0	38.0	41.0	33.0	41.0
44	37.87875228284611	40.0	38.0	41.0	33.0	41.0
45	37.79181002560992	40.0	38.0	41.0	33.0	41.0
46	37.73505967475869	40.0	38.0	41.0	33.0	41.0
47	37.666611329178146	40.0	37.0	41.0	33.0	41.0
48	37.84623664919031	40.0	38.0	41.0	33.0	41.0
49	37.90528375273261	40.0	38.0	41.0	33.0	41.0
50	37.86858163634342	40.0	38.0	41.0	33.0	41.0
51	36.34626650292211	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	178472.0
3	4471.0
4	3187.0
5	1946.0
6	1972.0
7	3120.0
8	8396.0
9	13368.0
10	15513.0
11	18492.0
12	24209.0
13	30948.0
14	36922.0
15	40813.0
16	42952.0
17	44161.0
18	45710.0
19	47602.0
20	50511.0
21	53765.0
22	59197.0
23	66975.0
24	77107.0
25	88960.0
26	105961.0
27	122294.0
28	141879.0
29	170803.0
30	209251.0
31	264097.0
32	344115.0
33	466070.0
34	685500.0
35	1052929.0
36	1638263.0
37	2697998.0
38	5972353.0
39	2.0929937E7
40	14203.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.373900046666098	27.02721591697525	28.884997512324713	23.713886524033942
2	22.531590504001283	28.329280519794953	30.180844765220126	18.95828421098364
3	22.392380117279306	27.081888163322493	30.03183412722339	20.49389759217481
4	21.977877146029094	25.66007427138448	30.773403061353438	21.588645521232984
5	25.877004575964495	25.836105633571393	28.12820616687685	20.15868362358726
6	22.179521427299367	25.501399862026286	31.44800273159166	20.871075979082683
7	24.91669550688226	26.131913932832994	28.585466054745357	20.365924505539386
8	22.474393823046505	26.010503792708434	30.750939747368573	20.76416263687649
9	22.474461063271267	25.95574024818833	30.842979827144738	20.726818861395664
10	22.510889169454803	26.944874419393273	29.874004503521444	20.670231907630473
11	25.153863383798036	25.951482574649916	28.03030805622988	20.864345985322174
12	21.881847323212316	29.08158074833416	28.08093572571003	20.955636202743495
13	23.695024250073175	26.314605976214384	28.640394142934028	21.349975630778417
14	24.038503858980583	26.591075207667924	27.909367483338027	21.461053450013466
15	23.396657757265356	27.43254683254844	27.529296644943873	21.641498765242325
16	23.67829473397399	26.776962299586305	27.74680408185471	21.797938884585
17	23.506949672870046	26.77361020100017	27.854621097146048	21.864819028983735
18	23.372333998915995	26.737082373012505	27.91342182123726	21.97716180683424
19	23.15377138555758	26.952970184317888	28.097976252916943	21.79528217720759
20	22.906065578373287	27.0085102124151	28.138663408267572	21.94676080094404
21	22.987496886358503	27.03647755582073	27.973517440707425	22.002508117113344
22	22.816266874810427	27.13449356103739	28.133062867913655	21.916176696238523
23	22.854498205684305	27.20969839441205	27.985469244623424	21.950334155280217
24	22.743922132790967	27.23874296556549	27.91785347601314	22.099481425630405
25	22.762919436138546	27.404671640790628	27.79422094986123	22.038187973209595
26	22.807330038188383	27.311196688762163	27.781174499573964	22.10029877347549
27	22.787748559275162	27.31942440773215	27.71235306917208	22.18047396382061
28	22.77663431828181	27.390479337096917	27.68513789222487	22.147748452396403
29	22.834572956785856	27.39856905631795	27.589212745966286	22.177645240929905
30	22.8661681431178	27.302293745604395	27.585812715789988	22.245725395487817
31	22.945596080377626	27.304976839228782	27.45394942926166	22.295477651131932
32	22.985351920836205	27.33662306481871	27.41616855290087	22.261856461444214
33	22.96019284624175	27.26456799939899	27.3717116705967	22.403527483762556
34	22.94183345163742	27.30277470065993	27.37865830967111	22.376733538031537
35	23.039594264621208	27.269544893981767	27.307122769702485	22.38373807169454
36	23.021401528811534	27.24460045706829	27.32570863265209	22.408289381468087
37	22.99296039569483	27.231689020217814	27.32813215849299	22.447218425594365
38	22.991294753173815	27.282301729709175	27.40969126719665	22.31671224992036
39	22.97607060389028	27.151172941207648	27.384303606754486	22.488452848147585
40	23.001332084206425	27.189028079251575	27.381308802025906	22.428331034516088
41	23.009283629060974	27.201494872546363	27.380659479417695	22.40856201897497
42	22.931721581720666	27.186548251857012	27.367795524242723	22.5139346421796
43	23.003902936144204	27.2259415702773	27.271686216568725	22.49846927700977
44	23.067619137867258	27.138488725243086	27.327630473722426	22.466261663167234
45	23.065836632094758	27.147330536934728	27.217203028420663	22.569629802549855
46	22.943493264462962	27.26893836801193	27.2111856787047	22.576382688820402
47	22.983690089553686	27.209175925284683	27.25228830945265	22.55484567570898
48	22.972804237974064	27.09944366695757	27.192588425257092	22.735163669811275
49	23.0435764770861	27.18828590389616	27.178672056717872	22.589465562299864
50	22.92476635633553	27.20060979108117	27.234547354341636	22.64007649824166
51	22.96594376511602	27.13292466106228	27.226440418588243	22.674691155233457
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.08778657070004
2	41.48987471498492
3	42.88627770945412
4	43.56652266726208
5	46.03568819955176
6	43.050597406382046
7	45.282620012421646
8	43.238556459923
9	43.20127992466693
10	43.18112107708528
11	46.018209369120214
12	42.83748352595581
13	45.04499988085159
14	45.49955730899405
15	45.038156522507684
16	45.476233618558986
17	45.37176870185378
18	45.34949580575024
19	44.949053562765165
20	44.85282637931733
21	44.99000500347184
22	44.732443571048954
23	44.804832360964525
24	44.84340355842137
25	44.80110740934815
26	44.907628811663876
27	44.96822252309577
28	44.924382770678214
29	45.01221819771576
30	45.11189353860562
31	45.24107373150956
32	45.24720838228043
33	45.363720330004305
34	45.31856698966896
35	45.423332336315745
36	45.42969091027962
37	45.44017882128919
38	45.30800700309417
39	45.46452345203787
40	45.429663118722516
41	45.41784564803594
42	45.445656223900265
43	45.50237221315398
44	45.533880801034485
45	45.63546643464462
46	45.51987595328337
47	45.538535765262665
48	45.70796790778534
49	45.63304203938596
50	45.56484285457719
51	45.640634920349484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189926.0
1	100430.5
2	10935.0
3	12306.5
4	13678.0
5	25883.5
6	38089.0
7	47007.5
8	55926.0
9	43904.0
10	31882.0
11	32796.5
12	33711.0
13	42418.5
14	51126.0
15	68901.5
16	86677.0
17	118398.5
18	150120.0
19	191031.5
20	231943.0
21	281943.5
22	331944.0
23	396436.5
24	460929.0
25	545880.0
26	737053.0
27	843275.0
28	932060.5
29	1020846.0
30	1108298.0
31	1195750.0
32	1302391.0
33	1409032.0
34	1509419.0
35	1609806.0
36	1751186.0
37	1892566.0
38	1995908.0
39	2099250.0
40	2204532.5
41	2309815.0
42	2348322.5
43	2386830.0
44	2407617.5
45	2428405.0
46	2418773.0
47	2409141.0
48	2368724.0
49	2328307.0
50	2240278.0
51	2152249.0
52	2009402.0
53	1866555.0
54	1732275.5
55	1597996.0
56	1468711.0
57	1339426.0
58	1195683.5
59	1051941.0
60	943041.0
61	834141.0
62	731620.5
63	629100.0
64	550409.5
65	471719.0
66	397837.5
67	323956.0
68	309663.5
69	295371.0
70	264259.0
71	233147.0
72	200623.0
73	168099.0
74	142143.5
75	99131.0
76	82074.0
77	76828.0
78	71582.0
79	73465.0
80	75348.0
81	66342.0
82	57336.0
83	52457.5
84	47579.0
85	42097.0
86	36615.0
87	42634.5
88	48654.0
89	32330.0
90	16006.0
91	10204.5
92	4403.0
93	3317.0
94	2231.0
95	1688.0
96	1145.0
97	897.0
98	649.0
99	410.5
100	172.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7698684831302096
2	0.5945784393106337
3	0.5643864770198104
4	0.5719142017165225
5	0.5629021763090959
6	0.5496385098828431
7	0.5484616914285855
8	0.5202991120303775
9	0.5446013914634316
10	0.5554415386501562
11	0.5627093010755002
12	0.5343873899625827
13	0.5270525404994664
14	0.5431254766324387
15	0.5653760108269534
16	0.5283942812549145
17	0.5172662188644166
18	0.5200782838643767
19	0.5530264052903496
20	0.541613222989319
21	0.5621977624124856
22	0.5885238341516741
23	0.5158182569658288
24	0.5395642730440201
25	0.537470598406873
26	0.5524505748828031
27	0.5555729174324606
28	0.5050591732830791
29	0.5625639458269934
30	0.5247827623881666
31	0.555564531552739
32	0.5479809009912167
33	0.5427313402855258
34	0.5338451030739224
35	0.5450150948630281
36	0.5513548199325204
37	0.5419262958322569
38	0.5395111624724503
39	0.5253502069159915
40	0.5194521381785009
41	0.5321819035958149
42	0.573971537541543
43	0.5309100451713797
44	0.5606603511302014
45	0.5419961781632698
46	0.550275836741681
47	0.5459710851512849
48	0.5581865166123439
49	0.5611103933419246
50	0.5598161725715652
51	0.5162850709369952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5774422E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.98041613424749
#Duplication Level	Relative count
1	100.0
2	13.248404212340757
3	4.232540601686013
4	2.0489374882167577
5	1.3520428775350803
6	0.9271728298634491
7	0.7285410326160144
8	0.5568423604190794
9	0.4369899539443562
10++	11.134153895876539
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	167704	0.4687818576076505	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11497280	3.9553192	4.929223	16
TGTGT	6660240	3.4067857	29.06501	4
AAAAA	7900240	3.2729683	3.5041888	10
TGTTT	6261970	2.626828	23.970102	6
GTTTG	4809485	2.4601042	28.409313	7
TTTGA	5406300	2.3537693	24.443079	8
CTTTG	4302435	2.3125682	30.057276	1
GTGTT	4451360	2.2769194	28.386566	5
TTGTG	4179835	2.1380315	28.429565	3
TTTGT	4949860	2.0764124	23.646833	2
GAGGG	2547575	2.010914	7.5440116	11
TTGAG	3377595	1.7931042	17.0899	9
TGAGG	2740010	1.7737173	9.996357	10
ACTTT	3212975	1.4699289	9.020359	3
AACTT	2913860	1.3835685	9.6264925	2
TGAGT	2255320	1.1973087	5.1498013	10
TTGAT	2564205	1.1163914	5.8073077	9
TAACT	2178140	1.0342315	9.428775	1
TTGAC	1689640	0.94257826	5.3001685	9
>>END_MODULE