FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_1_ACAGTG_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_1_ACAGTG_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 35774422
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 11073845 3.8389113 4.6886115 16
AAAAA 7589540 3.071283 3.2537317 12
TGTGT 5722940 2.9767087 29.06864 4
TGTTT 5463930 2.3201635 23.813234 6
CTTTG 3910235 2.1078963 29.360735 1
GTTTG 4004705 2.0829926 28.331743 7
TTTGA 4634920 2.0299933 24.181534 8
TTTGT 4552500 1.9331404 23.444473 2
GAGGG 2375105 1.9118173 7.5280476 11
GTGTT 3634645 1.8905108 28.311556 5
TTGTG 3405980 1.7715739 28.302185 3
TGAGG 2547160 1.673848 9.953265 10
TTGAG 2934925 1.5745379 16.843313 9
TGAGT 2169285 1.1637849 5.102827 10
TTGAT 2409625 1.0553629 5.8665905 9
TTGAC 1588055 0.8829801 5.217125 9
Produced by FastQC (version 0.10.1)