##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_1_ACAGTG_L002_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	35774422	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.012663321296984	34.0	33.0	34.0	31.0	34.0
2	33.19270547543717	34.0	34.0	34.0	31.0	34.0
3	33.23967531886329	34.0	34.0	34.0	31.0	34.0
4	36.547386985036404	37.0	37.0	37.0	35.0	37.0
5	36.485840553901895	37.0	37.0	37.0	35.0	37.0
6	36.477682518532376	37.0	37.0	37.0	35.0	37.0
7	36.474574040637194	37.0	37.0	37.0	35.0	37.0
8	36.451915812923545	37.0	37.0	37.0	35.0	37.0
9	38.273975887017826	39.0	39.0	39.0	37.0	39.0
10	38.26045916269451	39.0	39.0	39.0	37.0	39.0
11	38.261170704588885	39.0	39.0	39.0	37.0	39.0
12	38.22069404783116	39.0	39.0	39.0	37.0	39.0
13	38.18818115356273	39.0	39.0	39.0	37.0	39.0
14	39.75701815671543	41.0	40.0	41.0	38.0	41.0
15	39.73559986517741	41.0	40.0	41.0	38.0	41.0
16	39.718872411132175	41.0	40.0	41.0	38.0	41.0
17	39.687207105680145	41.0	40.0	41.0	38.0	41.0
18	39.68117164827988	41.0	40.0	41.0	37.0	41.0
19	39.670945682923964	41.0	40.0	41.0	37.0	41.0
20	39.596254664855245	41.0	40.0	41.0	37.0	41.0
21	39.58315200731964	41.0	40.0	41.0	37.0	41.0
22	39.52665155568411	41.0	40.0	41.0	37.0	41.0
23	39.51183183336966	41.0	39.0	41.0	37.0	41.0
24	39.49836654244197	41.0	39.0	41.0	37.0	41.0
25	39.457194612396535	41.0	39.0	41.0	37.0	41.0
26	39.36394877323245	41.0	39.0	41.0	36.0	41.0
27	39.3067524333447	41.0	39.0	41.0	36.0	41.0
28	39.25428100557432	41.0	39.0	41.0	36.0	41.0
29	39.20673765183404	40.0	39.0	41.0	36.0	41.0
30	39.12089964723958	40.0	39.0	41.0	36.0	41.0
31	39.0580462208446	40.0	39.0	41.0	36.0	41.0
32	38.985345255892604	40.0	39.0	41.0	35.0	41.0
33	38.904545879175906	40.0	38.0	41.0	35.0	41.0
34	38.83726781106345	40.0	38.0	41.0	35.0	41.0
35	38.76577371396804	40.0	38.0	41.0	35.0	41.0
36	38.697189768712406	40.0	38.0	41.0	35.0	41.0
37	38.875764337995456	40.0	38.0	41.0	35.0	41.0
38	38.89579529754527	40.0	39.0	41.0	35.0	41.0
39	38.8396924763732	40.0	39.0	41.0	35.0	41.0
40	38.805530414998735	40.0	38.0	41.0	35.0	41.0
41	38.77546703619698	40.0	38.0	41.0	35.0	41.0
42	38.7177936515648	40.0	38.0	41.0	35.0	41.0
43	38.67317565605951	40.0	38.0	41.0	35.0	41.0
44	38.60064643392422	40.0	38.0	41.0	35.0	41.0
45	38.54573530216645	40.0	38.0	41.0	35.0	41.0
46	38.47595142138145	40.0	38.0	41.0	35.0	41.0
47	38.37596258019207	40.0	38.0	41.0	34.0	41.0
48	38.32474827965075	40.0	38.0	41.0	34.0	41.0
49	38.27846018029306	40.0	38.0	41.0	34.0	41.0
50	38.3728145209446	40.0	38.0	41.0	34.0	41.0
51	38.104622263359	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	9.0
11	19.0
12	63.0
13	106.0
14	254.0
15	449.0
16	738.0
17	1138.0
18	2065.0
19	3572.0
20	6199.0
21	10461.0
22	16623.0
23	25281.0
24	36382.0
25	50783.0
26	69712.0
27	92897.0
28	122897.0
29	160658.0
30	209630.0
31	272484.0
32	358065.0
33	482519.0
34	709982.0
35	1092061.0
36	1698229.0
37	2846206.0
38	6416992.0
39	2.1067174E7
40	20767.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	20.916512260301047	27.451408182972898	27.771073551945346	23.86100600478071
2	22.681383052836797	27.29402117551127	30.438648450326607	19.585947321325335
3	21.44381656539594	26.16409619771185	31.388353450229197	21.003733786663012
4	22.06223940658778	26.050913341823644	30.960465274897047	20.92638197669153
5	25.32896011275997	26.439734918809815	27.87066885094754	20.360636117482674
6	22.621522718102895	26.122375925458698	30.02988559815166	21.226215758286745
7	25.113273686983455	26.855963211251304	27.222647044748406	20.808116057016836
8	21.996450424831462	26.56764377632712	30.165046412210376	21.27085938663104
9	21.67404968834996	26.49588859884305	30.59289958619038	21.23716212661661
10	21.999617492072968	26.59142613121744	30.240728976697373	21.16822740001222
11	24.783814272199407	26.477302217690607	27.48056064331264	21.258322866797343
12	21.893773565053777	29.470594200787087	27.310858175232262	21.324774058926877
13	23.545579576380018	26.877728450790904	27.93239818102442	21.644293791804657
14	23.134685446490234	27.00175002128616	28.12413852556444	21.739426006659173
15	23.20587877003296	27.032774980962653	27.87777256051824	21.88357368848615
16	23.170728502952585	26.97920282499391	27.87534139657159	21.97472727548191
17	23.14381207892052	27.030698078084953	27.79683484473907	22.028654998255455
18	22.987418217406837	26.96861461521307	27.91283392363404	22.13113324374605
19	22.819156498346608	27.178618443506974	28.088301041198626	21.913924016947785
20	22.625471346981403	27.261396991457854	28.10526585024992	22.00786581131082
21	22.721709154702758	27.204573826402196	27.93387221962221	22.139844799272844
22	22.68244054369348	27.26374447084009	27.99504908842413	22.058765897042306
23	22.74592165318562	27.27904590603868	27.871430040155502	22.103602400620197
24	22.607890072968896	27.29165826913989	27.87991375514047	22.220537902750742
25	22.67699729761974	27.434747451165343	27.74735377723303	22.140901473981888
26	22.694550764993622	27.349433181522798	27.718562897015563	22.237453156468018
27	22.678476039157598	27.33336762227525	27.652757935071463	22.33539840349568
28	22.691654324510417	27.400995706204277	27.65707541547578	22.250274553809525
29	22.737500329495365	27.413899821053615	27.59092304070036	22.25767680875066
30	22.768019362382816	27.31298941726987	27.589628829984658	22.32936239036266
31	22.87248990020049	27.314565165283106	27.452256477953053	22.36068845656335
32	22.88994532117329	27.37773787232401	27.41550331537821	22.316813491124492
33	22.807047801759094	27.281566012189966	27.41899135950323	22.492394826547706
34	22.80866613308125	27.35455560173701	27.41972801120222	22.41705025397952
35	22.89384307911092	27.310404778763935	27.35375477404122	22.44199736808392
36	22.943450358427516	27.262579526982293	27.342025095810023	22.451945018780165
37	22.92545441732183	27.2523731801314	27.353025722027404	22.469146680519366
38	22.913614611791477	27.31864508120962	27.417699167239583	22.350041139759316
39	22.907704840067016	27.20231790187973	27.36153780485957	22.52843945319368
40	22.910945389853463	27.231128490465128	27.419969585167635	22.437956534513773
41	22.90493703079884	27.21791535164903	27.431485257476506	22.445662360075623
42	22.82508807899938	27.240458400746398	27.396351597707497	22.538101922546723
43	22.908585814284645	27.27542854548822	27.299127068079056	22.51685857214808
44	22.976992125384253	27.15501738300133	27.369867047356905	22.49812344425751
45	22.952637221360398	27.116149843093485	27.288925349710443	22.64228758583568
46	22.874983591578225	27.283213720504257	27.27044757702119	22.57135511089633
47	22.872499564004002	27.320223385368948	27.25216635991575	22.555110690711302
48	22.867809503065693	27.128721522895816	27.260198713790523	22.743270260247968
49	22.997388652671518	27.18517700425499	27.239459060123494	22.577975282950003
50	22.85098683225726	27.1883951068056	27.276453441217228	22.68416461971991
51	22.898031750298454	27.18373976640056	27.25246110531274	22.665767377988246
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.77751826508175
2	42.26733037416213
3	42.447550352058954
4	42.98862138327931
5	45.68959623024264
6	43.847738476389644
7	45.921389744000294
8	43.2673098114625
9	42.91121181496657
10	43.16784489208519
11	46.04213713899675
12	43.218547623980655
13	45.18987336818468
14	44.8741114531494
15	45.089452458519105
16	45.1454557784345
17	45.17246707717597
18	45.11855146115289
19	44.7330805152944
20	44.63333715829222
21	44.8615539539756
22	44.74120644073579
23	44.849524053805816
24	44.828427975719634
25	44.81789877160163
26	44.932003921461636
27	45.01387444265328
28	44.94192887831994
29	44.99517713824602
30	45.09738175274548
31	45.233178356763844
32	45.20675881229778
33	45.2994426283068
34	45.22571638706077
35	45.33584044719484
36	45.39539537720768
37	45.394601097841196
38	45.26365575155079
39	45.436144293260696
40	45.34890192436723
41	45.35059939087447
42	45.3631900015461
43	45.42544438643273
44	45.47511556964176
45	45.59492480719607
46	45.446338702474556
47	45.427610254715304
48	45.61107976331366
49	45.575363935621525
50	45.53515145197717
51	45.563799128286696
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26136.0
1	16096.5
2	6057.0
3	8826.0
4	11595.0
5	17717.0
6	23839.0
7	36205.0
8	48571.0
9	38281.0
10	27991.0
11	30008.5
12	32026.0
13	41727.5
14	51429.0
15	71261.0
16	91093.0
17	125584.5
18	160076.0
19	203525.5
20	246975.0
21	298124.5
22	349274.0
23	416929.5
24	484585.0
25	573767.0
26	770387.5
27	877826.0
28	961691.0
29	1045556.0
30	1128118.5
31	1210681.0
32	1314069.0
33	1417457.0
34	1514397.5
35	1611338.0
36	1746696.5
37	1882055.0
38	1980273.5
39	2078492.0
40	2182438.5
41	2286385.0
42	2322301.0
43	2358217.0
44	2379435.0
45	2400653.0
46	2391891.5
47	2383130.0
48	2351454.5
49	2319779.0
50	2232921.0
51	2146063.0
52	2014869.5
53	1883676.0
54	1750748.5
55	1617821.0
56	1489139.0
57	1360457.0
58	1215356.0
59	1070255.0
60	961845.0
61	853435.0
62	747780.5
63	642126.0
64	562735.0
65	483344.0
66	408050.5
67	332757.0
68	319645.0
69	306533.0
70	274439.5
71	242346.0
72	208328.0
73	174310.0
74	147822.0
75	102400.0
76	83466.0
77	78306.0
78	73146.0
79	75875.5
80	78605.0
81	68628.5
82	58652.0
83	53022.5
84	47393.0
85	42459.0
86	37525.0
87	43729.0
88	49933.0
89	31739.5
90	13546.0
91	7965.5
92	2385.0
93	1653.0
94	921.0
95	538.5
96	156.0
97	106.0
98	56.0
99	36.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21811952685077624
2	0.011309756451131482
3	4.472469184827081E-5
4	8.441785586361116E-4
5	6.708703777240622E-5
6	0.0
7	1.6771759443101555E-5
8	0.0
9	0.0
10	0.0
11	2.5157639164652336E-4
12	1.397646620258463E-4
13	0.0
14	0.0
15	0.0
16	1.2299290258274473E-4
17	0.0
18	0.0
19	2.1244228627928635E-4
20	0.0017246959293989433
21	0.003175453121227228
22	0.0
23	0.0
24	0.0
25	9.503997017757547E-5
26	1.6771759443101555E-5
27	3.354351888620311E-5
28	3.913410536723696E-5
29	5.311057156982159E-5
30	0.0031838390009487783
31	5.506727683818344E-4
32	0.0027310014959850367
33	2.5157639164652336E-4
34	2.26418752481871E-4
35	0.0013557172216507088
36	6.792562574456129E-4
37	0.0051796783746778634
38	7.547291749395699E-5
39	0.0
40	0.0011991808001817611
41	5.227198359766651E-4
42	0.0015961124403351646
43	1.1181172962067703E-4
44	2.935057902542772E-4
45	7.100044830912991E-4
46	3.074822564568618E-4
47	3.074822564568618E-5
48	4.360657455206404E-4
49	4.724045576473604E-4
50	0.0011432749353714228
51	0.0026918673906177995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.5774422E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.88698226474055
#Duplication Level	Relative count
1	100.0
2	13.820191829871819
3	4.512562882485484
4	2.215097242360501
5	1.3995820314361374
6	0.9523420534918074
7	0.7147670880388833
8	0.5622834425905883
9	0.4826379670662555
10++	11.486647470064874
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	11073845	3.8389113	4.6886115	16
AAAAA	7589540	3.071283	3.2537317	12
TGTGT	5722940	2.9767087	29.06864	4
TGTTT	5463930	2.3201635	23.813234	6
CTTTG	3910235	2.1078963	29.360735	1
GTTTG	4004705	2.0829926	28.331743	7
TTTGA	4634920	2.0299933	24.181534	8
TTTGT	4552500	1.9331404	23.444473	2
GAGGG	2375105	1.9118173	7.5280476	11
GTGTT	3634645	1.8905108	28.311556	5
TTGTG	3405980	1.7715739	28.302185	3
TGAGG	2547160	1.673848	9.953265	10
TTGAG	2934925	1.5745379	16.843313	9
TGAGT	2169285	1.1637849	5.102827	10
TTGAT	2409625	1.0553629	5.8665905	9
TTGAC	1588055	0.8829801	5.217125	9
>>END_MODULE