FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_14_TGACCA_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_14_TGACCA_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 20892507
Filtered Sequences 0
Sequence length 51
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 96899 0.46379785824649955 No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30124 0.14418566426709825 No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCA 24958 0.11945909602902131 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23717 0.11351916742208103 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 10092440 5.789939 7.79181 17
TGTGT 5554375 4.341171 51.020653 4
AAAAA 4370175 4.137378 4.5329175 44
TTTGA 5119960 3.7896664 48.34736 8
GTTTG 4835425 3.779256 50.819218 7
TGTTT 5383990 3.605197 43.906487 6
GTGTT 4570805 3.5724351 50.73223 5
TTGTG 4076235 3.1858904 50.70595 3
CTTTG 3695265 3.1566968 55.61353 1
TTTGT 3964555 2.6547227 43.63302 2
TTGAG 3005790 2.5968084 31.479382 9
GAGGG 1955315 2.3013985 12.320443 11
AACTT 2346905 2.0987236 27.842546 2
ACTTT 2531895 2.0483105 24.43628 3
TGAGG 1988105 2.0047839 17.055468 10
TAACT 1762640 1.5762438 27.635504 1
AGGGG 1271790 1.4968922 6.124639 12
TGAGC 1352475 1.4906412 7.3548956 10
TTGAT 1963380 1.4532448 10.665794 9
TGAGA 1411420 1.347868 5.5760875 10
TTGAC 1364585 1.2885388 11.285001 9
TGAGT 1413950 1.2215617 8.423164 10
GAGTG 1158430 1.1681484 5.6051216 11
TGATT 1518415 1.1238929 5.1043744 10
GTAAC 831630 0.8680342 7.9879484 1
TGACG 576100 0.63495326 5.374183 10
Produced by FastQC (version 0.10.1)