##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_14_TGACCA_L002_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	20892507	
Filtered Sequences	0	
Sequence length	51	
%GC	46	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35266763342475	34.0	31.0	34.0	31.0	34.0
2	32.48448709386576	34.0	31.0	34.0	31.0	34.0
3	32.56524250536329	34.0	31.0	34.0	31.0	34.0
4	35.904499876438955	37.0	37.0	37.0	35.0	37.0
5	35.93320322927258	37.0	37.0	37.0	35.0	37.0
6	35.92686789574846	37.0	37.0	37.0	35.0	37.0
7	35.95328305980704	37.0	37.0	37.0	35.0	37.0
8	35.93521174840339	37.0	37.0	37.0	35.0	37.0
9	37.6947734419809	39.0	39.0	39.0	35.0	39.0
10	37.670057116649524	39.0	39.0	39.0	35.0	39.0
11	37.67676564617162	39.0	39.0	39.0	35.0	39.0
12	37.62389161817679	39.0	38.0	39.0	35.0	39.0
13	37.63253375959142	39.0	38.0	39.0	35.0	39.0
14	39.14223147083306	41.0	40.0	41.0	36.0	41.0
15	39.09249124578491	41.0	40.0	41.0	36.0	41.0
16	39.07213021395661	41.0	40.0	41.0	36.0	41.0
17	39.04582676459077	41.0	40.0	41.0	36.0	41.0
18	39.01329313901869	41.0	39.0	41.0	36.0	41.0
19	39.00071571113988	41.0	40.0	41.0	36.0	41.0
20	38.95968243543008	41.0	39.0	41.0	36.0	41.0
21	38.92355656504028	41.0	39.0	41.0	36.0	41.0
22	38.86822596254245	41.0	39.0	41.0	36.0	41.0
23	38.84070470815207	41.0	39.0	41.0	36.0	41.0
24	38.799152921188444	41.0	39.0	41.0	35.0	41.0
25	38.72258681066853	41.0	39.0	41.0	35.0	41.0
26	38.67808793841735	41.0	39.0	41.0	35.0	41.0
27	38.61997696111816	41.0	39.0	41.0	35.0	41.0
28	38.5749368421894	41.0	39.0	41.0	35.0	41.0
29	38.51333451748993	41.0	39.0	41.0	35.0	41.0
30	38.46368767520336	40.0	39.0	41.0	35.0	41.0
31	38.37918044014536	40.0	38.0	41.0	34.0	41.0
32	38.3260364110444	40.0	38.0	41.0	34.0	41.0
33	38.237950045918375	40.0	38.0	41.0	34.0	41.0
34	38.20742833782466	40.0	38.0	41.0	34.0	41.0
35	38.12700486351399	40.0	38.0	41.0	34.0	41.0
36	38.05992871032662	40.0	38.0	41.0	33.0	41.0
37	37.99589211577146	40.0	38.0	41.0	33.0	41.0
38	37.92468701817355	40.0	38.0	41.0	33.0	41.0
39	37.85148464949659	40.0	38.0	41.0	33.0	41.0
40	37.76577827639354	40.0	38.0	41.0	33.0	41.0
41	37.6800692229037	40.0	37.0	41.0	33.0	41.0
42	37.583220386141306	40.0	37.0	41.0	33.0	41.0
43	37.50644731146913	40.0	37.0	41.0	32.0	41.0
44	37.4140672538724	40.0	37.0	41.0	32.0	41.0
45	37.308753994913104	40.0	37.0	41.0	32.0	41.0
46	37.226131885464966	40.0	36.0	41.0	31.0	41.0
47	37.13816269153338	40.0	36.0	41.0	31.0	41.0
48	37.32475949391808	40.0	36.0	41.0	32.0	41.0
49	37.37655256020735	40.0	36.0	41.0	32.0	41.0
50	37.31905218459422	40.0	36.0	41.0	32.0	41.0
51	35.779956613153225	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103110.0
3	2685.0
4	1883.0
5	1135.0
6	1169.0
7	2351.0
8	7196.0
9	11874.0
10	12329.0
11	13919.0
12	17455.0
13	21858.0
14	26217.0
15	28460.0
16	29240.0
17	30118.0
18	30665.0
19	31946.0
20	33923.0
21	36189.0
22	39766.0
23	44246.0
24	51295.0
25	59193.0
26	70896.0
27	82243.0
28	95767.0
29	115683.0
30	141307.0
31	177575.0
32	229466.0
33	310469.0
34	458044.0
35	720096.0
36	1144240.0
37	1796119.0
38	3692596.0
39	1.1212794E7
40	6990.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.063403195404902	23.480896780058988	31.30708099940051	25.148619025135595
2	21.818413890398457	26.828111572040342	33.032157842618155	18.321316694943047
3	23.62727305326476	25.975073336720296	31.053827431940466	19.34382617807448
4	21.649920398404145	22.570139203751058	33.555428998279154	22.224511399565642
5	29.981621556941914	22.291309513283398	28.362505896836304	19.36456303293839
6	20.877134800403045	21.905996135754215	37.49601454836032	19.720854515482415
7	27.207980841391404	22.39305794004532	31.383170150655264	19.01579106790801
8	24.080835134282513	22.723367133395524	33.937888386424106	19.257909345897854
9	24.17516519506849	23.351374679759203	33.12092065343079	19.352539471741522
10	23.88452954402423	25.814474158664837	30.881679129928713	19.41931716738222
11	29.10167126829964	22.858132462531934	27.980611050522917	20.059585218645513
12	22.268242839112133	29.30278572937433	28.267788599648057	20.161182831865474
13	25.87529897558011	23.450824140530912	29.347755692384382	21.326121191504594
14	27.330173213094067	24.077335666472937	26.991285038299544	21.60120608213345
15	25.369008254756487	26.67646221861454	26.095193472900856	21.859336053728118
16	26.162936499947747	24.80095908399703	26.737839339573128	22.2982650764821
17	25.521221751203477	24.924746612328637	27.174946427651314	22.379085208816573
18	25.398210558510183	24.82645952803958	27.354951216797986	22.42037869665225
19	24.834238214822808	25.28696532207674	27.843884203853726	22.03491225924673
20	24.157801817327602	25.39155727977464	28.05167376610137	22.398967136796387
21	24.431674030381153	25.56350549743206	27.688537138549417	22.316283333637372
22	23.732591309760164	25.84852552809167	28.295942883468545	22.122940278679625
23	23.764708848180305	26.134462643417706	27.911970429025835	22.188858079376157
24	23.47804997295586	26.1601263471544	27.892294055485728	22.469529624404018
25	23.4053958749655	26.630781040255215	27.679281204442013	22.284541880337276
26	23.523475622125538	26.420019904705942	27.58679239643891	22.469712076729614
27	23.45735614643863	26.456959465696745	27.519815507358135	22.565868880506496
28	23.470229044428045	26.65397444677746	27.450599894756913	22.425196614037585
29	23.654372745278774	26.457396448280097	27.204735305655365	22.68349550078577
30	23.712048625334862	26.43715341559124	27.150320887833455	22.70047707124044
31	23.76109929788171	26.368100228164497	27.003714056222115	22.867086417731674
32	23.88750585142034	26.50440395929707	26.812270361679495	22.795819827603093
33	23.923519692953853	26.311307220878877	26.800133145981903	22.965039940185367
34	23.973790576658416	26.355044615311403	26.777699018347185	22.893465789683002
35	24.09465261394226	26.220675152918854	26.62648087205327	23.058191361085616
36	24.07748762710686	26.244264144033064	26.739983593448642	22.93826463541144
37	23.951886634375246	26.103252026025757	26.843509475158683	23.10135186444032
38	23.913321431628855	26.252131958299728	26.99603580751576	22.83851080255565
39	23.888376818524065	26.0358935958786	26.927457364789408	23.14827222080793
40	23.974360621252025	26.13840547674173	26.954703376112622	22.932530525893625
41	23.93594638833036	26.09572232502585	26.947060487555273	23.021270799088516
42	23.799064331381466	26.06805062234296	26.9736137136405	23.159271332635075
43	23.903823642514908	26.22912830943589	26.74546651695237	23.121581531096833
44	24.030079781104938	25.963985585917214	26.860803448228726	23.145131184749122
45	24.05986131619921	26.012079191101794	26.696085463368906	23.23197402933009
46	23.78200925747946	26.31561115121143	26.55115816671914	23.351221424589966
47	23.937892455234596	26.131320189571998	26.727972457390575	23.202814897802828
48	23.835538914951933	25.90565505482882	26.556670995382003	23.702135034837248
49	23.953031209075895	26.113689159899007	26.628454470928943	23.304825160096158
50	23.727410326093366	26.141150136502954	26.708185744519774	23.4232537928839
51	23.865904148809236	26.02721707843455	26.618022323906455	23.48885644884976
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	45.2120222205405
2	40.139730585341496
3	42.97109923133924
4	43.874431797969784
5	49.3461845898803
6	40.597989315885464
7	46.223771909299415
8	43.33874448018037
9	43.52770466681001
10	43.30384671140645
11	49.16125648694515
12	42.42942567097761
13	47.201420167084706
14	48.931379295227515
15	47.2283443084846
16	48.46120157642984
17	47.90030696002005
18	47.81858925516243
19	46.869150474069535
20	46.55676895412399
21	46.747957364018525
22	45.85553158843979
23	45.95356692755646
24	45.947579597359876
25	45.68993775530277
26	45.993187698855145
27	46.02322502694513
28	45.895425658465626
29	46.33786824606454
30	46.4125256965753
31	46.628185715613384
32	46.68332567902343
33	46.888559633139224
34	46.867256366341415
35	47.15284397502788
36	47.0157522625183
37	47.05323849881557
38	46.751832234184505
39	47.03664903933199
40	46.90689114714565
41	46.95721718741888
42	46.95833566401654
43	47.02540517361174
44	47.175210965854056
45	47.29183534552931
46	47.133230682069424
47	47.14070735303743
48	47.53767394978918
49	47.25785636917205
50	47.15066411897727
51	47.354760597658995
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116856.0
1	61896.5
2	6937.0
3	7753.5
4	8570.0
5	31252.0
6	53934.0
7	64886.0
8	75838.0
9	52626.0
10	29414.0
11	25016.0
12	20618.0
13	22776.0
14	24934.0
15	33793.0
16	42652.0
17	62591.0
18	82530.0
19	102976.0
20	123422.0
21	147067.5
22	170713.0
23	203477.0
24	236241.0
25	276287.0
26	375187.5
27	434042.0
28	474779.5
29	515517.0
30	552905.0
31	590293.0
32	647169.5
33	704046.0
34	749680.0
35	795314.0
36	893560.0
37	991806.0
38	1056104.0
39	1120402.0
40	1196314.0
41	1272226.0
42	1299139.5
43	1326053.0
44	1350559.5
45	1375066.0
46	1376827.0
47	1378588.0
48	1372567.5
49	1366547.0
50	1333661.0
51	1300775.0
52	1217510.0
53	1134245.0
54	1059878.0
55	985511.0
56	918354.5
57	851198.0
58	771048.0
59	690898.0
60	632517.0
61	574136.0
62	513852.5
63	453569.0
64	405494.0
65	357419.0
66	304440.0
67	251461.0
68	249244.5
69	247028.0
70	224373.5
71	201719.0
72	174925.0
73	148131.0
74	129049.5
75	92506.5
76	75045.0
77	70554.0
78	66063.0
79	66797.5
80	67532.0
81	59586.5
82	51641.0
83	48023.0
84	44405.0
85	39823.0
86	35241.0
87	39952.0
88	44663.0
89	29560.0
90	14457.0
91	9336.5
92	4216.0
93	3248.0
94	2280.0
95	1733.5
96	1187.0
97	907.0
98	627.0
99	413.5
100	200.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.7648579464398408
2	0.5892543197424799
3	0.5588702207925549
4	0.5668347987151566
5	0.5577884932622016
6	0.5447311804179364
7	0.5434723559025252
8	0.5151799159382835
9	0.5400548627314089
10	0.5512741960550737
11	0.557850716527222
12	0.528979121557791
13	0.5218952421554771
14	0.5384514170558852
15	0.5604449480380693
16	0.5233598820859555
17	0.5124516650873924
18	0.5149501684982085
19	0.5487517606192498
20	0.5371016508454443
21	0.5577789204521985
22	0.5835489249806163
23	0.510800355361853
24	0.5344834873095891
25	0.5327220902690136
26	0.5480720911090278
27	0.5508817108449455
28	0.499911283983296
29	0.5583676482673908
30	0.5198466608148079
31	0.5502164005497282
32	0.5426060165972423
33	0.5375515729155912
34	0.5289743351527895
35	0.5401888820714528
36	0.5467127520885837
37	0.536886262620374
38	0.5347850308246875
39	0.5202247868099313
40	0.5142896446079928
41	0.5272990934022422
42	0.569165777950918
43	0.5255999196266873
44	0.5559840185766122
45	0.5367570296853317
46	0.5459612865033383
47	0.5411844543117779
48	0.5540263789309727
49	0.5563860765967434
50	0.5553857179514167
51	0.5112263454069921
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0892507E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	43.66159066122712
#Duplication Level	Relative count
1	100.0
2	13.937064892419539
3	4.735547796410878
4	2.463488603855833
5	1.654753113416549
6	1.129935401535737
7	0.8804318991661708
8	0.7485105071086989
9	0.6273435763602602
10++	17.215741663500076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	96899	0.46379785824649955	No Hit
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30124	0.14418566426709825	No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCA	24958	0.11945909602902131	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23717	0.11351916742208103	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	10092440	5.789939	7.79181	17
TGTGT	5554375	4.341171	51.020653	4
AAAAA	4370175	4.137378	4.5329175	44
TTTGA	5119960	3.7896664	48.34736	8
GTTTG	4835425	3.779256	50.819218	7
TGTTT	5383990	3.605197	43.906487	6
GTGTT	4570805	3.5724351	50.73223	5
TTGTG	4076235	3.1858904	50.70595	3
CTTTG	3695265	3.1566968	55.61353	1
TTTGT	3964555	2.6547227	43.63302	2
TTGAG	3005790	2.5968084	31.479382	9
GAGGG	1955315	2.3013985	12.320443	11
AACTT	2346905	2.0987236	27.842546	2
ACTTT	2531895	2.0483105	24.43628	3
TGAGG	1988105	2.0047839	17.055468	10
TAACT	1762640	1.5762438	27.635504	1
AGGGG	1271790	1.4968922	6.124639	12
TGAGC	1352475	1.4906412	7.3548956	10
TTGAT	1963380	1.4532448	10.665794	9
TGAGA	1411420	1.347868	5.5760875	10
TTGAC	1364585	1.2885388	11.285001	9
TGAGT	1413950	1.2215617	8.423164	10
GAGTG	1158430	1.1681484	5.6051216	11
TGATT	1518415	1.1238929	5.1043744	10
GTAAC	831630	0.8680342	7.9879484	1
TGACG	576100	0.63495326	5.374183	10
>>END_MODULE