FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_14_TGACCA_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_14_TGACCA_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 20892507
Filtered Sequences 0
Sequence length 51
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34899 0.16704074814956385 No Hit
CTTTGTGTTTGACGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCA 24787 0.11864062077375395 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 8504030 5.4878235 7.081896 18
AAAAA 4618945 4.103625 4.5515127 47
TGTGT 3984350 3.388609 55.550877 4
TTTGA 3782485 2.9872167 51.592567 8
TGTTT 3997605 2.9615533 48.539074 6
GTTTG 3450915 2.9349332 55.23611 7
CTTTG 3036210 2.727411 58.08661 1
GTGTT 3174730 2.7000437 55.16902 5
TTTGT 3252925 2.4098704 48.131348 2
TTGTG 2706460 2.301789 55.05566 3
GAGGG 1714250 2.0483139 12.030679 11
TTGAG 2241265 2.0320153 32.58116 9
TGAGG 1663985 1.7319188 17.497517 10
TGAGC 1266115 1.3918941 7.2946453 10
AGGGG 1120295 1.3386121 5.958892 12
TTGAT 1679235 1.3261755 11.699215 9
TGAGA 1330215 1.2856584 5.5994577 10
TGAGT 1248980 1.1323726 8.735266 10
TGATT 1406745 1.1109768 5.7527366 10
TTGAC 1159050 1.1099179 11.697569 9
GAGTG 1065420 1.1089168 5.6878786 11
TGACG 495970 0.5452409 5.4336553 10
Produced by FastQC (version 0.10.1)