FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_12_CAGATC_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_12_CAGATC_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34775151
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 9495565 4.5537047 46.0403 4
TTTTT 13327335 4.234609 5.560808 18
AAAAA 7660110 3.499263 3.6776595 43
GTTTG 7112260 3.410764 45.484642 7
TGTTT 8505475 3.320134 37.544735 6
GTGTT 6802105 3.262026 45.452267 5
TTTGA 7610970 3.1947095 40.007156 8
CTTTG 5743450 3.0096004 49.793285 1
TTGTG 6251340 2.9979002 45.46004 3
GGGGG 3005060 2.6721342 5.683897 13
TTTGT 6483920 2.5310147 37.239655 2
TTGAG 4629505 2.3873327 28.172224 9
GAGGG 2966750 2.3090503 12.166154 11
TGAGG 3292540 2.0859144 16.26188 10
ACTTT 3851125 1.7663263 19.31075 3
AACTT 3473450 1.7130839 21.396702 2
AGGGG 2143035 1.6679449 6.6826086 12
TGAGC 2170095 1.5022289 6.486358 10
TGAGA 2636590 1.4620267 5.2631855 10
TAACT 2715305 1.3391716 21.249712 1
TGAGT 2531780 1.3055826 7.9149117 10
GAGTG 2037435 1.2907709 6.0571346 11
TTGAT 2970205 1.2467455 8.947303 9
TTGAC 1978745 1.1149622 8.68537 9
GTAAC 1258200 0.7623498 6.2821393 1
Produced by FastQC (version 0.10.1)