##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_12_CAGATC_L007_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34775151	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12444314044819	34.0	31.0	34.0	31.0	34.0
2	32.28868317494869	34.0	31.0	34.0	31.0	34.0
3	32.37325689829499	34.0	31.0	34.0	31.0	34.0
4	35.75730822851064	37.0	37.0	37.0	35.0	37.0
5	35.7779145804428	37.0	37.0	37.0	35.0	37.0
6	35.7770175606139	37.0	36.0	37.0	35.0	37.0
7	35.81639283176657	37.0	37.0	37.0	35.0	37.0
8	35.81411174893245	37.0	37.0	37.0	35.0	37.0
9	37.54631115764242	39.0	38.0	39.0	35.0	39.0
10	37.521734585710355	39.0	38.0	39.0	35.0	39.0
11	37.51308881448135	39.0	38.0	39.0	35.0	39.0
12	37.47069285191601	39.0	38.0	39.0	35.0	39.0
13	37.466831071416486	39.0	38.0	39.0	35.0	39.0
14	38.93321484067747	41.0	39.0	41.0	36.0	41.0
15	38.876042177358194	41.0	39.0	41.0	36.0	41.0
16	38.847819266119075	41.0	39.0	41.0	36.0	41.0
17	38.816914698659396	41.0	39.0	41.0	36.0	41.0
18	38.781519252065934	41.0	39.0	41.0	36.0	41.0
19	38.7886936853272	41.0	39.0	41.0	35.0	41.0
20	38.7514633078085	41.0	39.0	41.0	35.0	41.0
21	38.71359140899201	41.0	39.0	41.0	35.0	41.0
22	38.66623656069818	41.0	39.0	41.0	35.0	41.0
23	38.63666717076225	41.0	39.0	41.0	35.0	41.0
24	38.60108095576638	41.0	39.0	41.0	35.0	41.0
25	38.553523434017585	41.0	39.0	41.0	34.0	41.0
26	38.5130121793001	41.0	39.0	41.0	34.0	41.0
27	38.45529441410621	40.0	39.0	41.0	34.0	41.0
28	38.4171716177451	40.0	39.0	41.0	34.0	41.0
29	38.352466564415494	40.0	38.0	41.0	34.0	41.0
30	38.30647803657273	40.0	38.0	41.0	34.0	41.0
31	38.23648219385158	40.0	38.0	41.0	34.0	41.0
32	38.18834704700492	40.0	38.0	41.0	34.0	41.0
33	38.11759833911289	40.0	38.0	41.0	33.0	41.0
34	38.07290234340032	40.0	38.0	41.0	33.0	41.0
35	38.02379368532433	40.0	38.0	41.0	33.0	41.0
36	37.967976041283045	40.0	38.0	41.0	33.0	41.0
37	37.91506159096189	40.0	38.0	41.0	33.0	41.0
38	37.85557212965085	40.0	38.0	41.0	33.0	41.0
39	37.79137718194236	40.0	38.0	41.0	33.0	41.0
40	37.719689297682706	40.0	38.0	41.0	33.0	41.0
41	37.64655161382333	40.0	38.0	41.0	32.0	41.0
42	37.56682707143385	40.0	38.0	41.0	32.0	41.0
43	37.51436558823281	40.0	37.0	41.0	32.0	41.0
44	37.43835323676955	40.0	37.0	41.0	32.0	41.0
45	37.368321765159266	40.0	37.0	41.0	31.0	41.0
46	37.30320676393325	40.0	37.0	41.0	31.0	41.0
47	37.222690851867185	40.0	37.0	41.0	31.0	41.0
48	37.44930657526117	40.0	37.0	41.0	32.0	41.0
49	37.477795998642826	40.0	37.0	41.0	32.0	41.0
50	37.45385427657812	40.0	37.0	41.0	32.0	41.0
51	35.94361784367234	38.0	35.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	130307.0
3	3780.0
4	2014.0
5	1927.0
6	2075.0
7	4504.0
8	11989.0
9	19307.0
10	21035.0
11	24650.0
12	32383.0
13	41346.0
14	49203.0
15	53834.0
16	56081.0
17	57898.0
18	59591.0
19	61464.0
20	65341.0
21	70376.0
22	77618.0
23	87138.0
24	98760.0
25	115427.0
26	140295.0
27	157678.0
28	182654.0
29	219832.0
30	269077.0
31	336139.0
32	433585.0
33	578833.0
34	826583.0
35	1232317.0
36	1853203.0
37	2962968.0
38	6284016.0
39	1.8141424E7
40	8499.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.437810900394023	25.19222065819769	30.999969166843144	24.369999274565142
2	21.33436150308646	27.839458939876373	32.64122708513095	18.184952471906218
3	22.606529304199974	26.947881347422964	31.37166994115741	19.073919407219655
4	21.30492158133248	24.076480173353964	33.12053811208763	21.498060133225923
5	28.287875321243238	23.952784171643653	28.688320850196757	19.071019656916352
6	20.790211469849588	23.586558894934228	36.13468612944392	19.488543505772267
7	26.17572800246759	24.028319639536093	30.923145900106242	18.872806457890075
8	23.177122137855427	24.227678573232733	33.43877530813791	19.156423980773923
9	23.00099009606625	24.694432211325484	33.12876851544734	19.17580917716092
10	23.04324720071069	26.569862840171865	31.18688411615339	19.20000584296405
11	27.534432378576152	24.38751579609276	28.471662885035627	19.60638894029546
12	21.58299545842882	29.994706827822505	28.682579948311083	19.739717765437597
13	24.78791447856291	24.964430352404108	29.674595970318794	20.573059198714187
14	25.960555726148097	25.498195251455897	27.72941681969246	20.811832202703542
15	24.38481279070624	27.665462774223887	26.93300831654656	21.016716118523316
16	25.01258162904638	26.073825982828367	27.498249685044335	21.415342703080913
17	24.597581322521332	26.142021465351856	27.80509432561489	21.455302886511923
18	24.33295846361086	26.103897393879667	27.982596450799534	21.580547691709942
19	23.871816816056786	26.514907543250928	28.348611723060657	21.26466391763163
20	23.43762889781581	26.577837757285632	28.47422320879517	21.51031013610339
21	23.50732218675948	26.735712865320487	28.258637020644688	21.498327927275344
22	23.090247934533732	26.987169312620246	28.584610374598707	21.337972378247315
23	23.101718823256714	27.13367015092215	28.360185187484916	21.404425838336223
24	22.883966189395718	27.214582218180556	28.305624783444216	21.595826808979513
25	22.90491197435516	27.42744036847857	28.152979652159626	21.514668005006644
26	22.90577613435421	27.34135960385754	28.1156944785501	21.637169783238146
27	22.89905124869095	27.381399157889536	28.012924269758493	21.706625323661022
28	22.862154138402346	27.482476609377887	27.96903722987807	21.6863320223417
29	23.002402266747747	27.391749628951544	27.806043391897955	21.799804712402754
30	23.004627758215882	27.369040321502247	27.779188015501767	21.847143904780104
31	23.086951229298254	27.32113175615464	27.669527336877586	21.922389677669525
32	23.15128057746534	27.406304142754813	27.5560275837258	21.88638769605405
33	23.102707799274704	27.310489110777723	27.564079238157024	22.02272385179055
34	23.123198483726963	27.350860620372003	27.531585051347317	21.994355844553716
35	23.213059371965862	27.300941641122854	27.43357020202283	22.05242878488846
36	23.170253566683996	27.282469820366202	27.49721596488188	22.050060648067927
37	23.122270867820685	27.21898480231629	27.528346245266505	22.130398084596525
38	23.10051979964851	27.285242244633025	27.614862415301317	21.999375540417155
39	23.053108538872483	27.159976990822692	27.587560063628963	22.199354406675862
40	23.088790167912073	27.205301649334825	27.60905465084858	22.09685353190452
41	23.11133395148722	27.197904722858603	27.5863129569549	22.104448368699277
42	22.992634458058276	27.183698769856047	27.575469824260697	22.248196947824987
43	23.091027363419574	27.256315787361522	27.459292433643046	22.193364415575857
44	23.146496050339195	27.124430713838827	27.516795853467755	22.212277382354216
45	23.119113293708924	27.15020741816259	27.441365725101736	22.289313563026745
46	23.023926204848674	27.296393484203413	27.34150828265426	22.338172028293656
47	23.072455921366082	27.20318950241681	27.42670522683769	22.29764934937942
48	22.99361461598964	27.072491818821497	27.35864932110983	22.57524424407903
49	23.06665028946284	27.200450850709462	27.370736024355967	22.36216283547173
50	22.972136505491356	27.192912931641594	27.401453135196984	22.433497427670062
51	23.014021606607624	27.151343984226283	27.361390255054452	22.473244154111637
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	43.807810174959165
2	39.519313974992684
3	41.68044871141963
4	42.8029817145584
5	47.3588949781596
6	40.27875497562185
7	45.048534460357665
8	42.33354611862935
9	42.176799273227175
10	42.24325304367474
11	47.14082131887161
12	41.32271322386642
13	45.360973677277094
14	46.77238792885164
15	45.40152890922955
16	46.4279243321273
17	46.05288420903326
18	45.9135061553208
19	45.136480733688416
20	44.9479390339192
21	45.00565011403482
22	44.42822031278105
23	44.506144661592934
24	44.47979299837523
25	44.41957997936181
26	44.542945917592355
27	44.60567657235197
28	44.54848616074404
29	44.802206979150505
30	44.851771662995986
31	45.00934090696778
32	45.03766827351939
33	45.12543165106525
34	45.117554328280676
35	45.26548815685432
36	45.22031421475192
37	45.25266895241721
38	45.09989534006566
39	45.252462945548345
40	45.185643699816595
41	45.215782320186506
42	45.240831405883256
43	45.28439177899543
44	45.35877343269341
45	45.40842685673567
46	45.362098233142326
47	45.370105270745505
48	45.56885886006867
49	45.42881312493457
50	45.40563393316142
51	45.48726576071927
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	146014.0
1	77595.5
2	9177.0
3	10306.5
4	11436.0
5	32375.5
6	53315.0
7	67516.0
8	81717.0
9	60152.5
10	38588.0
11	36487.5
12	34387.0
13	41148.5
14	47910.0
15	63898.0
16	79886.0
17	109603.0
18	139320.0
19	176783.5
20	214247.0
21	262587.5
22	310928.0
23	373901.0
24	436874.0
25	516004.0
26	695650.0
27	796166.0
28	886414.5
29	976663.0
30	1068475.5
31	1160288.0
32	1269375.5
33	1378463.0
34	1481545.0
35	1584627.0
36	1730810.5
37	1876994.0
38	1984069.0
39	2091144.0
40	2197078.5
41	2303013.0
42	2349942.5
43	2396872.0
44	2418694.5
45	2440517.0
46	2423353.5
47	2406190.0
48	2354857.0
49	2303524.0
50	2209071.0
51	2114618.0
52	1966695.5
53	1818773.0
54	1675249.5
55	1531726.0
56	1397471.5
57	1263217.0
58	1122372.0
59	981527.0
60	874193.5
61	766860.0
62	668334.5
63	569809.0
64	494578.5
65	419348.0
66	355416.0
67	291484.0
68	276307.5
69	261131.0
70	234015.0
71	206899.0
72	178117.5
73	149336.0
74	126032.5
75	86755.0
76	70781.0
77	67134.0
78	63487.0
79	63210.5
80	62934.0
81	55586.0
82	48238.0
83	43992.5
84	39747.0
85	35622.0
86	31497.0
87	37042.5
88	42588.0
89	28825.0
90	15062.0
91	9936.0
92	4810.0
93	3741.0
94	2672.0
95	2043.5
96	1415.0
97	1113.0
98	811.0
99	534.5
100	258.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8606087720510546
2	0.49622789560281133
3	0.47941128997541954
4	0.48719845961272745
5	0.48367007809685714
6	0.43808005319660576
7	0.4134503973828899
8	0.38255189747414753
9	0.37681504244223124
10	0.3887776073208137
11	0.39804571948515766
12	0.41335262641994
13	0.3953196349887884
14	0.3679121335806708
15	0.4232792547759174
16	0.37781575700418957
17	0.41621098927794736
18	0.4102785923201311
19	0.4115582416881525
20	0.41001115998029747
21	0.4369671895889108
22	0.4641733978380137
23	0.42986154107569513
24	0.4416573201939511
25	0.4174992654956408
26	0.40297740188101555
27	0.3896891777694941
28	0.3866323973690294
29	0.4263734181916277
30	0.4125934636487991
31	0.44991033971355004
32	0.4370419556194019
33	0.4279003705835813
34	0.41082783508258525
35	0.42665810423080547
36	0.4289010851455397
37	0.41262797104748733
38	0.4221663911682224
39	0.4133756313523987
40	0.42570627515032217
41	0.42981553121077753
42	0.45359688014007477
43	0.3987157381430206
44	0.4401533727344563
45	0.43529645636908953
46	0.4126481003633888
47	0.4257551606317971
48	0.42678750697588635
49	0.4541374960528568
50	0.4428708303811535
51	0.4297522676465157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4775151E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.677658781421755
#Duplication Level	Relative count
1	100.0
2	11.031765095399157
3	3.5063004343112367
4	1.7327005849244013
5	1.0802599375474724
6	0.7816303875043009
7	0.5816784279101774
8	0.45248868778280543
9	0.36614579613988846
10++	10.30596544985945
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	9495565	4.5537047	46.0403	4
TTTTT	13327335	4.234609	5.560808	18
AAAAA	7660110	3.499263	3.6776595	43
GTTTG	7112260	3.410764	45.484642	7
TGTTT	8505475	3.320134	37.544735	6
GTGTT	6802105	3.262026	45.452267	5
TTTGA	7610970	3.1947095	40.007156	8
CTTTG	5743450	3.0096004	49.793285	1
TTGTG	6251340	2.9979002	45.46004	3
GGGGG	3005060	2.6721342	5.683897	13
TTTGT	6483920	2.5310147	37.239655	2
TTGAG	4629505	2.3873327	28.172224	9
GAGGG	2966750	2.3090503	12.166154	11
TGAGG	3292540	2.0859144	16.26188	10
ACTTT	3851125	1.7663263	19.31075	3
AACTT	3473450	1.7130839	21.396702	2
AGGGG	2143035	1.6679449	6.6826086	12
TGAGC	2170095	1.5022289	6.486358	10
TGAGA	2636590	1.4620267	5.2631855	10
TAACT	2715305	1.3391716	21.249712	1
TGAGT	2531780	1.3055826	7.9149117	10
GAGTG	2037435	1.2907709	6.0571346	11
TTGAT	2970205	1.2467455	8.947303	9
TTGAC	1978745	1.1149622	8.68537	9
GTAAC	1258200	0.7623498	6.2821393	1
>>END_MODULE