FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_12_CAGATC_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_12_CAGATC_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 34775151
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38247 0.10998370646902439 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12134085 4.0910363 5.202901 18
TGTGT 7261570 3.7000568 48.08881 4
AAAAA 7577010 3.295878 3.432467 44
TGTTT 6629620 2.7478354 39.11452 6
GTTTG 5211140 2.6552815 47.35654 7
CTTTG 4810375 2.5895305 49.97908 1
TTTGA 5783525 2.5224586 40.818058 8
GTGTT 4894500 2.493941 47.348038 5
TTTGT 5468025 2.266379 38.738586 2
TTGTG 4381090 2.2323384 47.327324 3
GAGGG 2573575 2.0854297 12.058115 11
TTGAG 3560675 1.909148 28.356714 9
TGAGG 2812440 1.8538141 16.48291 10
AGGGG 1878245 1.5219871 6.570871 12
TGAGC 2056090 1.431823 6.402751 10
TGAGA 2513790 1.4182917 5.291282 10
GAGTG 1883185 1.2412977 6.0551496 11
TGAGT 2293235 1.2295771 7.986811 10
TTGAT 2596875 1.1326156 9.471875 9
TTGAC 1738105 0.98457205 8.661533 9
Produced by FastQC (version 0.10.1)