##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_12_CAGATC_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	34775151	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88426221355588	34.0	31.0	34.0	31.0	34.0
2	33.07458167471365	34.0	33.0	34.0	31.0	34.0
3	33.11826168634034	34.0	33.0	34.0	31.0	34.0
4	36.474522598047095	37.0	37.0	37.0	35.0	37.0
5	36.383827779784475	37.0	37.0	37.0	35.0	37.0
6	36.34886876551593	37.0	37.0	37.0	35.0	37.0
7	36.359798581464105	37.0	37.0	37.0	35.0	37.0
8	36.33584699603461	37.0	37.0	37.0	35.0	37.0
9	38.12971483574579	39.0	38.0	39.0	37.0	39.0
10	38.09791094796397	39.0	38.0	39.0	37.0	39.0
11	38.085763653477734	39.0	38.0	39.0	37.0	39.0
12	38.0467514576716	39.0	38.0	39.0	37.0	39.0
13	38.04554844348483	39.0	38.0	39.0	37.0	39.0
14	39.54641237934524	41.0	40.0	41.0	37.0	41.0
15	39.53297315660829	41.0	39.0	41.0	37.0	41.0
16	39.49208674895473	41.0	39.0	41.0	37.0	41.0
17	39.4703487268826	41.0	39.0	41.0	37.0	41.0
18	39.431377911198716	41.0	39.0	41.0	37.0	41.0
19	39.41737098424102	41.0	39.0	41.0	37.0	41.0
20	39.37722660068392	41.0	39.0	41.0	36.0	41.0
21	39.34995718638289	41.0	39.0	41.0	36.0	41.0
22	39.30401242542412	41.0	39.0	41.0	36.0	41.0
23	39.264222346583054	40.0	39.0	41.0	36.0	41.0
24	39.22936121830212	41.0	39.0	41.0	36.0	41.0
25	39.18254488672098	40.0	39.0	41.0	36.0	41.0
26	39.08786153078099	40.0	39.0	41.0	36.0	41.0
27	39.023634548704045	40.0	39.0	41.0	36.0	41.0
28	38.96919216828131	40.0	39.0	41.0	35.0	41.0
29	38.89068056095572	40.0	38.0	41.0	35.0	41.0
30	38.836336239057594	40.0	38.0	41.0	35.0	41.0
31	38.743884850420926	40.0	38.0	41.0	35.0	41.0
32	38.66684561628503	40.0	38.0	41.0	35.0	41.0
33	38.558443096336234	40.0	38.0	41.0	35.0	41.0
34	38.50501301921018	40.0	38.0	41.0	34.0	41.0
35	38.43390667088692	40.0	38.0	41.0	34.0	41.0
36	38.36421722510996	40.0	38.0	41.0	34.0	41.0
37	38.55964547788736	40.0	38.0	41.0	35.0	41.0
38	38.58046689718184	40.0	38.0	41.0	35.0	41.0
39	38.577411870907476	40.0	38.0	41.0	35.0	41.0
40	38.53873080234792	40.0	38.0	41.0	35.0	41.0
41	38.49435779588707	40.0	38.0	41.0	34.0	41.0
42	38.415629194536066	40.0	38.0	41.0	34.0	41.0
43	38.362762738255256	40.0	38.0	41.0	34.0	41.0
44	38.285269674314286	40.0	38.0	41.0	34.0	41.0
45	38.22693569324832	40.0	38.0	41.0	34.0	41.0
46	38.15994271311719	40.0	38.0	41.0	34.0	41.0
47	38.07383387062791	40.0	38.0	41.0	33.0	41.0
48	38.019323654410584	40.0	37.0	41.0	33.0	41.0
49	37.966975528014245	40.0	37.0	41.0	33.0	41.0
50	38.0567239233555	40.0	37.0	41.0	33.0	41.0
51	37.7895171181284	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	11.0
11	22.0
12	57.0
13	141.0
14	257.0
15	454.0
16	736.0
17	1300.0
18	2322.0
19	4151.0
20	7267.0
21	12623.0
22	20239.0
23	31204.0
24	45348.0
25	64060.0
26	87814.0
27	116330.0
28	154671.0
29	203125.0
30	264589.0
31	340681.0
32	441434.0
33	590232.0
34	843347.0
35	1260164.0
36	1897900.0
37	3097010.0
38	6689992.0
39	1.8585441E7
40	12227.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.501065753778583	26.48110363186742	27.906676186170117	25.11115442818388
2	22.26663236626843	26.28533877721765	31.946883355904333	19.501145500609596
3	20.99598359759818	25.20922770400048	33.529516521725526	20.26527217667581
4	22.06657564190016	25.10754590253253	32.66799330360924	20.157885151958073
5	27.02330178235603	25.471251009089794	27.798671528414065	19.706775680140108
6	21.95161424317036	25.05494800008201	32.40024752157079	20.593190235176838
7	26.958466031059537	25.67415525166678	27.284768002438526	20.082610714835162
8	22.043357913816102	25.716817160621385	31.821581450501824	20.41824347506068
9	21.212698688209866	26.123041133595652	32.21762286524651	20.44663731294797
10	21.8217830312225	25.715385103575827	32.03022468543703	20.432607179764652
11	26.842994872976973	25.581266347340954	27.032175935052017	20.543562844630063
12	21.737314156466656	30.993241242925752	26.61678392322505	20.652660677382542
13	24.493233688618634	26.222074492214283	27.896272829987133	21.388418989179947
14	23.69389232472578	26.494852647735158	28.248527978431383	21.562727049107682
15	23.942826301458766	26.48584042093735	27.887893283339015	21.68343999426487
16	23.846818644557235	26.506867864767557	27.790114813608547	21.85619867706666
17	23.685741792220647	26.70942402000923	27.69700857356499	21.90782561420513
18	23.440373521798524	26.63620028730455	27.991101064286372	21.932325126610554
19	23.084716862661104	27.01960753413269	28.295461231625467	21.60021437158074
20	22.74700690731724	27.054539605018533	28.42551855490146	21.772934932762766
21	22.915374915708487	27.056001196256524	28.15416468368936	21.87445920434563
22	22.763288648264965	27.15064558598178	28.275624166232955	21.8104415995203
23	22.88205448770014	27.221348370277386	28.05519377902917	21.841403362993304
24	22.63271888763515	27.2819831018926	28.088691641962477	21.996606368509777
25	22.725577812731856	27.459066964223965	27.92590030737753	21.88945491566665
26	22.72512453341488	27.321060196648023	27.884482270038724	22.069332999898368
27	22.754523477636308	27.341534783425903	27.777390192262608	22.126551546675174
28	22.74014934978984	27.45304756636357	27.791076389884374	22.01572669396222
29	22.879089727029513	27.40118080292448	27.66088636106857	22.05884310897744
30	22.816780867464818	27.319634068591103	27.745955725684702	22.117629338259377
31	22.96623603453569	27.284465667966373	27.51761114828448	22.231687149213453
32	22.974118425995773	27.39966524792152	27.490804152664982	22.135412173417727
33	22.877654306123198	27.297587245004284	27.49583804730868	22.32892040156384
34	22.86658079500503	27.3551479330744	27.505122839006507	22.273148432914066
35	22.967307696455418	27.348227348575314	27.428366017488905	22.256098937480363
36	23.070478411283695	27.278312405615925	27.410177644087106	22.241031539013274
37	23.02052967653008	27.263166349068484	27.428830990520137	22.287472983881297
38	22.952516380231287	27.265380595051354	27.580016189721686	22.20208683499568
39	22.873052937508866	27.14851423194507	27.49736463567386	22.4810681948722
40	22.908004051513682	27.271772306610547	27.540653957189143	22.27956968468663
41	22.95975834388089	27.166494584405164	27.5745085518965	22.29923851981745
42	22.85542184161454	27.23990341493576	27.493788739769307	22.410886003680385
43	22.937565470665863	27.30603975555394	27.406666307022526	22.349728466757668
44	23.006574435866575	27.12408926707464	27.45346238755369	22.415873909505095
45	23.040219108424438	27.068758254466584	27.368136154385354	22.52288648272362
46	22.887224846270257	27.257210184364116	27.401715667604147	22.45384930176148
47	22.875851782785933	27.314549978517704	27.37781354277944	22.43178469591692
48	22.852179707285813	27.123327228686943	27.371642469647362	22.652850594379878
49	23.039247191191205	27.14529693918511	27.359599387505174	22.45585648211851
50	22.860659900513443	27.13451050147848	27.374075241254886	22.630754356753187
51	22.921676593974276	27.16669692776347	27.307257182304063	22.604369295958186
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	45.61222018196246
2	41.76777786687802
3	41.26125577427399
4	42.224460793858235
5	46.73007746249614
6	42.544804478347196
7	47.0410767458947
8	42.46160138887679
9	41.65933600115784
10	42.254390210987154
11	47.38655771760703
12	42.389974833849195
13	45.88165267779858
14	45.25661937383346
15	45.62626629572363
16	45.703017321623896
17	45.59356740642578
18	45.37269864840908
19	44.68493123424185
20	44.51994184008
21	44.78983412005412
22	44.573730247785264
23	44.72345785069344
24	44.62932525614493
25	44.6150327283985
26	44.794457533313256
27	44.881075024311485
28	44.75587604375206
29	44.93793283600695
30	44.9344102057242
31	45.19792318374915
32	45.1095305994135
33	45.20657470768704
34	45.1397292279191
35	45.22340663393578
36	45.311509950296966
37	45.30800266041138
38	45.15460321522696
39	45.35412113238107
40	45.18757373620031
41	45.25899686369834
42	45.26630784529493
43	45.28729393742353
44	45.42244834537167
45	45.56310559114806
46	45.34107414803174
47	45.307636478702854
48	45.50503030166569
49	45.495103673309714
50	45.491414257266634
51	45.52604588993246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30910.0
1	18407.5
2	5905.0
3	7958.5
4	10012.0
5	19974.0
6	29936.0
7	49765.5
8	69595.0
9	50966.0
10	32337.0
11	32563.0
12	32789.0
13	40447.5
14	48106.0
15	66154.5
16	84203.0
17	117461.0
18	150719.0
19	191529.5
20	232340.0
21	280400.0
22	328460.0
23	393993.0
24	459526.0
25	541442.5
26	727872.0
27	832385.0
28	913116.0
29	993847.0
30	1077856.0
31	1161865.0
32	1265341.5
33	1368818.0
34	1463618.5
35	1558419.0
36	1700928.5
37	1843438.0
38	1941789.5
39	2040141.0
40	2143820.5
41	2247500.0
42	2292165.0
43	2336830.0
44	2361144.0
45	2385458.0
46	2370225.0
47	2354992.0
48	2322076.0
49	2289160.0
50	2198436.0
51	2107712.0
52	1982978.5
53	1858245.0
54	1717476.5
55	1576708.0
56	1441149.5
57	1305591.0
58	1163279.0
59	1020967.0
60	914389.5
61	807812.0
62	702313.5
63	596815.0
64	521022.0
65	445229.0
66	376879.0
67	308529.0
68	296344.0
69	284159.0
70	255450.5
71	226742.0
72	195996.0
73	165250.0
74	138797.0
75	93539.0
76	74734.0
77	72046.0
78	69358.0
79	69850.0
80	70342.0
81	61460.0
82	52578.0
83	47133.0
84	41688.0
85	38078.0
86	34468.0
87	41260.5
88	48053.0
89	30637.5
90	13222.0
91	7780.0
92	2338.0
93	1641.0
94	944.0
95	569.5
96	195.0
97	124.5
98	54.0
99	39.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27453798834690896
2	0.022484445861931702
3	0.0
4	0.0
5	0.0
6	0.0
7	2.875616557351541E-6
8	0.0
9	0.0
10	0.0
11	0.0
12	7.6491400425551E-4
13	0.0
14	5.319890631100351E-4
15	0.0
16	1.4378082786757706E-5
17	5.751233114703082E-6
18	5.923770108144174E-4
19	0.005420537210607655
20	0.0
21	2.875616557351541E-6
22	0.0
23	0.0
24	4.025863180292158E-5
25	0.0
26	0.005040955825037252
27	0.004207027023405305
28	2.8756165573515412E-5
29	0.0
30	0.0
31	0.00332133712374103
32	0.0021825929670298196
33	2.3004932458812328E-5
34	0.0
35	0.004819533350121183
36	0.002912999572597111
37	2.2429809147342023E-4
38	2.875616557351541E-6
39	2.099200086866625E-4
40	0.0
41	3.3357152065277877E-4
42	5.751233114703082E-6
43	1.0064657950730393E-4
44	0.0
45	1.1214904573671011E-4
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	8.339288016319469E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.4775151E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.300276733716085
#Duplication Level	Relative count
1	100.0
2	11.973004115226338
3	3.7188477366255146
4	1.8818106995884774
5	1.1404115226337448
6	0.8079012345679012
7	0.6156378600823045
8	0.48987654320987656
9	0.42403292181069957
10++	10.635720164609053
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38247	0.10998370646902439	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	12134085	4.0910363	5.202901	18
TGTGT	7261570	3.7000568	48.08881	4
AAAAA	7577010	3.295878	3.432467	44
TGTTT	6629620	2.7478354	39.11452	6
GTTTG	5211140	2.6552815	47.35654	7
CTTTG	4810375	2.5895305	49.97908	1
TTTGA	5783525	2.5224586	40.818058	8
GTGTT	4894500	2.493941	47.348038	5
TTTGT	5468025	2.266379	38.738586	2
TTGTG	4381090	2.2323384	47.327324	3
GAGGG	2573575	2.0854297	12.058115	11
TTGAG	3560675	1.909148	28.356714	9
TGAGG	2812440	1.8538141	16.48291	10
AGGGG	1878245	1.5219871	6.570871	12
TGAGC	2056090	1.431823	6.402751	10
TGAGA	2513790	1.4182917	5.291282	10
GAGTG	1883185	1.2412977	6.0551496	11
TGAGT	2293235	1.2295771	7.986811	10
TTGAT	2596875	1.1326156	9.471875	9
TTGAC	1738105	0.98457205	8.661533	9
>>END_MODULE