FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_10_ACTTGA_L007_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_10_ACTTGA_L007_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 38097996
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 9040680 4.1296597 37.642567 4
TTTTT 12543020 3.6222484 4.629211 18
AAAAA 8301425 3.0962448 3.2155762 10
TGTTT 8080640 2.9348803 30.042213 6
GTTTG 6372125 2.9107 37.021973 7
GTGTT 6059895 2.7680779 36.978928 5
CTTTG 5447680 2.6716015 39.834545 1
TTTGA 6970445 2.6645658 31.175259 8
TTGTG 5686525 2.5975273 37.01211 3
GGGGG 2783955 2.5297692 5.236898 13
TTTGT 6308510 2.2912445 29.702868 2
GAGGG 2907640 2.2111192 10.585115 11
TTGAG 4390945 2.1110206 22.986597 9
TGAGG 3326170 2.0111637 13.798611 10
AGGGG 2208005 1.6790807 6.0402665 12
ACTTT 3760450 1.5433084 13.294392 3
TGAGC 2278280 1.4789621 5.3223124 10
AACTT 3381385 1.4605919 14.377586 2
GAGTG 2156250 1.3037733 5.3318305 11
TGAGT 2708135 1.3019814 6.7429757 10
TAACT 2617840 1.1307778 14.224715 1
TTGAT 2939455 1.1236544 7.1277204 9
TTGAC 1917885 0.98992884 6.37162 9
Produced by FastQC (version 0.10.1)