##FastQC 0.10.1 >>Basic Statistics pass #Measure Value Filename 140701_JD_C_10_ACTTGA_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 38097996 Filtered Sequences 0 Sequence length 51 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17775116045474 34.0 31.0 34.0 31.0 34.0 2 32.34292089799159 34.0 31.0 34.0 31.0 34.0 3 32.42606275143711 34.0 31.0 34.0 31.0 34.0 4 35.82029773954515 37.0 37.0 37.0 35.0 37.0 5 35.83296809102505 37.0 37.0 37.0 35.0 37.0 6 35.84229031364274 37.0 37.0 37.0 35.0 37.0 7 35.86819466829699 37.0 37.0 37.0 35.0 37.0 8 35.86985793163504 37.0 37.0 37.0 35.0 37.0 9 37.61490882617553 39.0 38.0 39.0 35.0 39.0 10 37.586228446241634 39.0 38.0 39.0 35.0 39.0 11 37.579609331682434 39.0 38.0 39.0 35.0 39.0 12 37.54040569482972 39.0 38.0 39.0 35.0 39.0 13 37.53210071731857 39.0 38.0 39.0 35.0 39.0 14 39.01540605967831 41.0 39.0 41.0 36.0 41.0 15 38.96445185725779 41.0 39.0 41.0 36.0 41.0 16 38.935349355383416 41.0 39.0 41.0 36.0 41.0 17 38.90860322416959 41.0 39.0 41.0 36.0 41.0 18 38.87733843008436 41.0 39.0 41.0 36.0 41.0 19 38.88890665010307 41.0 39.0 41.0 36.0 41.0 20 38.85536205106431 41.0 39.0 41.0 36.0 41.0 21 38.81816571139333 41.0 39.0 41.0 35.0 41.0 22 38.775239254054206 41.0 39.0 41.0 35.0 41.0 23 38.7486494302745 41.0 39.0 41.0 35.0 41.0 24 38.71750970838466 41.0 39.0 41.0 35.0 41.0 25 38.67336350184928 41.0 39.0 41.0 35.0 41.0 26 38.63751421465843 41.0 39.0 41.0 35.0 41.0 27 38.58234084018488 41.0 39.0 41.0 35.0 41.0 28 38.54945412351873 40.0 39.0 41.0 34.0 41.0 29 38.48808231278097 40.0 39.0 41.0 34.0 41.0 30 38.44742316629988 40.0 39.0 41.0 34.0 41.0 31 38.382598733014724 40.0 38.0 41.0 34.0 41.0 32 38.337888481063416 40.0 38.0 41.0 34.0 41.0 33 38.27227957082047 40.0 38.0 41.0 34.0 41.0 34 38.23147981326892 40.0 38.0 41.0 34.0 41.0 35 38.18991198382193 40.0 38.0 41.0 34.0 41.0 36 38.14003303480845 40.0 38.0 41.0 34.0 41.0 37 38.09258161505398 40.0 38.0 41.0 33.0 41.0 38 38.03878665953978 40.0 38.0 41.0 33.0 41.0 39 37.981923957365105 40.0 38.0 41.0 33.0 41.0 40 37.917945369095 40.0 38.0 41.0 33.0 41.0 41 37.853047047409 40.0 38.0 41.0 33.0 41.0 42 37.78136584926934 40.0 38.0 41.0 33.0 41.0 43 37.736325999929235 40.0 38.0 41.0 33.0 41.0 44 37.66789463151815 40.0 38.0 41.0 32.0 41.0 45 37.60435092176502 40.0 38.0 41.0 32.0 41.0 46 37.54805095260129 40.0 37.0 41.0 32.0 41.0 47 37.47505380597972 40.0 37.0 41.0 32.0 41.0 48 37.6974095697842 40.0 38.0 41.0 33.0 41.0 49 37.73012633000434 40.0 38.0 41.0 33.0 41.0 50 37.712549631219446 40.0 38.0 41.0 33.0 41.0 51 36.20870533452731 38.0 35.0 40.0 30.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 143904.0 3 4100.0 4 2309.0 5 1987.0 6 2089.0 7 4135.0 8 9772.0 9 15179.0 10 16309.0 11 20522.0 12 29012.0 13 39001.0 14 47456.0 15 53571.0 16 56430.0 17 58467.0 18 59833.0 19 63294.0 20 67304.0 21 72422.0 22 79612.0 23 89375.0 24 100885.0 25 118294.0 26 144625.0 27 161898.0 28 187238.0 29 224954.0 30 275602.0 31 345575.0 32 446414.0 33 597258.0 34 849641.0 35 1260358.0 36 1896042.0 37 3091760.0 38 6765070.0 39 2.0686073E7 40 10226.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 19.37783107694429 26.548669450098966 30.350844640696085 23.72265483226066 2 21.506451674342564 28.397950671621068 31.6530509234582 18.442546730578172 3 22.19156734794233 27.372589548664557 31.015054326454898 19.420788776938213 4 21.420611101410085 25.249010715033705 32.14633293257247 21.184045250983747 5 26.68741105853635 25.301298439863334 28.660386991377212 19.350903510223098 6 21.25160943573777 24.998139346361274 33.87529610625238 19.87495511164858 7 25.24947513219252 25.462498200756343 29.934949418091456 19.35307724895968 8 22.528769208380083 25.541632162816768 32.273698990218435 19.655899638584714 9 22.363413214369192 25.733303925337697 32.23045357441918 19.672829285873934 10 22.45100536653472 27.068747577815387 30.845736284436853 19.63451077121304 11 26.009361753691323 25.584857927243103 28.51637442492459 19.889405894140978 12 21.429366491240913 29.911176758306325 28.64754410224677 20.011912648206 13 23.937241312845963 26.051154091349343 29.44539624588756 20.56620834991713 14 24.62613101765889 26.454973247706743 28.16700093311476 20.751894801519605 15 23.595307796522512 27.90389104509618 27.56831000460121 20.9324911537801 16 23.999551127419526 26.82286375015071 27.966529745950137 21.211055376479628 17 23.71768092439298 26.884282999233726 28.163828487865683 21.234207588507605 18 23.481294543077635 26.858023552481765 28.283955530721546 21.37672637371906 19 23.16063134314133 27.179248328527173 28.539976814034045 21.120143514297453 20 22.84337346349093 27.22374412230229 28.602080774307897 21.330801639898876 21 22.894552724850314 27.296907296619406 28.46332070273331 21.34521927579697 22 22.65751268719614 27.492671436693765 28.62774695860667 21.222068917503425 23 22.654195279442323 27.564093540149365 28.47412677486204 21.30758440554627 24 22.502889370564898 27.643385179066097 28.419763452855566 21.433961997513435 25 22.54278778875217 27.780364760116623 28.292468648032138 21.38437880309907 26 22.523393496195336 27.711587759595517 28.27576415556497 21.489254588644176 27 22.52257627012881 27.748262024976288 28.175810178823003 21.553351526071904 28 22.493098820276995 27.805455810143915 28.1519621079192 21.549483261659898 29 22.571566109103575 27.766348879771602 28.04993739083333 21.61214762029149 30 22.57367521720757 27.731957162415537 28.031918725268916 21.66244889510797 31 22.65322004711045 27.708577532977912 27.949682755245675 21.688519664665957 32 22.678547512662625 27.753589985056887 27.87709211474032 21.690770387540166 33 22.64905476821096 27.707562212829995 27.846983400843612 21.796399618115434 34 22.644416723782545 27.730853318092592 27.843783035355546 21.780946922769314 35 22.729383066283184 27.69106591454817 27.763332683791788 21.81621833537686 36 22.6845528526869 27.688802001123914 27.79865218282223 21.827992963366956 37 22.68154447315174 27.665255047339272 27.789120208642977 21.86408027086602 38 22.649041211860425 27.706799098421193 27.8523569543937 21.791802735324683 39 22.63423236847551 27.61093159776815 27.82478370780823 21.930052325948107 40 22.630561785040264 27.653031821147795 27.85124590675339 21.865160487058553 41 22.679889176444913 27.64639981487404 27.823888646459103 21.849822362221946 42 22.617579022021665 27.627194013138663 27.79711493310641 21.95811203173327 43 22.65552400832102 27.678377561759675 27.743398217387256 21.92270021253205 44 22.68763352112966 27.60427467987226 27.778603874198872 21.92948792479921 45 22.682684439719175 27.593894664567358 27.709229548318458 22.014191347395005 46 22.596457579160816 27.69900158306216 27.67587017746369 22.02867066031333 47 22.663602633469313 27.628121972597686 27.707110235338313 22.001165158594695 48 22.595623425370647 27.562252064910925 27.66333993456691 22.178784575151518 49 22.624350057418894 27.649038033429395 27.663675230679562 22.06293667847214 50 22.557848097925078 27.643061021674477 27.696314073093042 22.1027768073074 51 22.625112075397656 27.59348421430332 27.651499404123076 22.12990430617595 >>END_MODULE >>Per base GC content pass #Base %GC 1 43.10048590920495 2 39.94899840492073 3 41.61235612488055 4 42.604656352393825 5 46.03831456875945 6 41.126564547386344 7 44.6025523811522 8 42.184668846964804 9 42.03624250024313 10 42.08551613774776 11 45.89876764783231 12 41.441279139446905 13 44.503449662763096 14 45.37802581917849 15 44.52779895030261 16 45.21060650389915 17 44.95188851290058 18 44.85802091679669 19 44.28077485743878 20 44.174175103389814 21 44.239772000647285 22 43.87958160469957 23 43.96177968498859 24 43.93685136807834 25 43.92716659185124 26 44.01264808483951 27 44.07592779620071 28 44.042582081936885 29 44.183713729395066 30 44.23612411231554 31 44.34173971177641 32 44.369317900202795 33 44.44545438632639 34 44.425363646551865 35 44.545601401660036 36 44.51254581605386 37 44.54562474401775 38 44.4408439471851 39 44.56428469442362 40 44.49572227209882 41 44.52971153866686 42 44.57569105375493 43 44.57822422085307 44 44.61712144592887 45 44.69687578711418 46 44.62512823947415 47 44.664767792064005 48 44.774408000522165 49 44.687286735891036 50 44.66062490523248 51 44.755016381573604 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 156543.0 1 83039.0 2 9535.0 3 10462.5 4 11390.0 5 25435.0 6 39480.0 7 50907.0 8 62334.0 9 48401.5 10 34469.0 11 35165.5 12 35862.0 13 45342.0 14 54822.0 15 73164.0 16 91506.0 17 125032.0 18 158558.0 19 202758.0 20 246958.0 21 302304.0 22 357650.0 23 431906.5 24 506163.0 25 599373.0 26 808000.0 27 923417.0 28 1030366.5 29 1137316.0 30 1246406.5 31 1355497.0 32 1479095.5 33 1602694.0 34 1723657.0 35 1844620.0 36 1996038.0 37 2147456.0 38 2261720.5 39 2375985.0 40 2483881.0 41 2591777.0 42 2635812.0 43 2679847.0 44 2697979.0 45 2716111.0 46 2688089.0 47 2660067.0 48 2589666.0 49 2519265.0 50 2402827.0 51 2286389.0 52 2121380.0 53 1956371.0 54 1792871.0 55 1629371.0 56 1472784.5 57 1316198.0 58 1162939.5 59 1009681.0 60 888319.0 61 766957.0 62 659780.5 63 552604.0 64 475442.0 65 398280.0 66 332423.0 67 266566.0 68 245327.5 69 224089.0 70 198193.0 71 172297.0 72 146291.5 73 120286.0 74 101727.0 75 70698.5 76 58229.0 77 54648.5 78 51068.0 79 50628.5 80 50189.0 81 43930.5 82 37672.0 83 34582.5 84 31493.0 85 27883.0 86 24273.0 87 28106.0 88 31939.0 89 21726.0 90 11513.0 91 7547.0 92 3581.0 93 2821.5 94 2062.0 95 1560.0 96 1058.0 97 813.0 98 568.0 99 378.5 100 189.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.8630375203987107 2 0.49913911482378237 3 0.48238495274134624 4 0.4902515082420608 5 0.4866922659134092 6 0.440382743491285 7 0.4158827671670709 8 0.3856292073735322 9 0.37954489784712037 10 0.3918815047384645 11 0.4009528480185677 12 0.4160035084260075 13 0.3985065251201139 14 0.3707544092345435 15 0.42600928405788063 16 0.38094392156479834 17 0.4193370170966473 18 0.41260962912589944 19 0.4142055135918435 20 0.41241014356765643 21 0.43929344735087905 22 0.4668906994478135 23 0.4324794406508941 24 0.44443282528561345 25 0.4204578109567758 26 0.4056486330672091 27 0.39246683736330906 28 0.38932493982098165 29 0.42903831477120213 30 0.4150743256941914 31 0.45207364712831616 32 0.43959267568824356 33 0.4305213324081403 34 0.4136280553969296 35 0.42928242209905215 36 0.43181536372674295 37 0.41564653426915155 38 0.4248989894376597 39 0.41653896966129134 40 0.4285133527758258 41 0.4328442892376806 42 0.45641245802010166 43 0.4019397765698752 44 0.4425587109621199 45 0.4375925704858597 46 0.41533680669187956 47 0.42848972948603387 48 0.4296078985361855 49 0.4568980478658248 50 0.44530688700791504 51 0.43263955405948384 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3.8097996E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Duplicate Percentage 24.776309063722653 #Duplication Level Relative count 1 100.0 2 11.748642555488521 3 3.286444606738005 4 1.5571714349220462 5 0.9195694281268854 6 0.6147397834439399 7 0.45470421998539357 8 0.35055409138538723 9 0.2800622360524561 10++ 7.800463595084622 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Kmer Content fail #Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position TGTGT 9040680 4.1296597 37.642567 4 TTTTT 12543020 3.6222484 4.629211 18 AAAAA 8301425 3.0962448 3.2155762 10 TGTTT 8080640 2.9348803 30.042213 6 GTTTG 6372125 2.9107 37.021973 7 GTGTT 6059895 2.7680779 36.978928 5 CTTTG 5447680 2.6716015 39.834545 1 TTTGA 6970445 2.6645658 31.175259 8 TTGTG 5686525 2.5975273 37.01211 3 GGGGG 2783955 2.5297692 5.236898 13 TTTGT 6308510 2.2912445 29.702868 2 GAGGG 2907640 2.2111192 10.585115 11 TTGAG 4390945 2.1110206 22.986597 9 TGAGG 3326170 2.0111637 13.798611 10 AGGGG 2208005 1.6790807 6.0402665 12 ACTTT 3760450 1.5433084 13.294392 3 TGAGC 2278280 1.4789621 5.3223124 10 AACTT 3381385 1.4605919 14.377586 2 GAGTG 2156250 1.3037733 5.3318305 11 TGAGT 2708135 1.3019814 6.7429757 10 TAACT 2617840 1.1307778 14.224715 1 TTGAT 2939455 1.1236544 7.1277204 9 TTGAC 1917885 0.98992884 6.37162 9 >>END_MODULE