FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_C_10_ACTTGA_L007_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_C_10_ACTTGA_L007_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 38097996
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TGTGT 7349540 3.485191 38.21453 4
TTTTT 11679195 3.4674737 4.2964754 16
TGTTT 6691375 2.5107226 30.24558 6
GTTTG 4965455 2.3546453 37.37214 7
CTTTG 4733330 2.3539288 39.196316 1
GTGTT 4639535 2.2000923 37.37961 5
TTTGA 5613610 2.1880107 30.959557 8
TTTGT 5550765 2.0827456 29.900513 2
TTGTG 4305280 2.0415869 37.391083 3
GAGGG 2586160 2.034754 10.518686 11
TGAGG 2974675 1.8518803 13.874398 10
TTGAG 3595375 1.7710649 22.899397 9
AGGGG 1971620 1.5512426 5.964935 12
TGAGC 2202725 1.4381155 5.2241993 10
GAGTG 2027925 1.2624822 5.436007 11
TGAGT 2519440 1.2410644 6.8125854 10
TTGAT 2669010 1.0402972 7.206736 9
TTGAC 1743975 0.90092915 6.2265196 9
Produced by FastQC (version 0.10.1)