##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_C_10_ACTTGA_L007_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	38097996	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.898933450462856	34.0	31.0	34.0	31.0	34.0
2	33.08733065644713	34.0	33.0	34.0	31.0	34.0
3	33.13485163366598	34.0	33.0	34.0	31.0	34.0
4	36.48504955483748	37.0	37.0	37.0	35.0	37.0
5	36.39547857057888	37.0	37.0	37.0	35.0	37.0
6	36.37187491436558	37.0	37.0	37.0	35.0	37.0
7	36.37356555447168	37.0	37.0	37.0	35.0	37.0
8	36.354727923222	37.0	37.0	37.0	35.0	37.0
9	38.146628919799355	39.0	39.0	39.0	37.0	39.0
10	38.112765931310406	39.0	38.0	39.0	37.0	39.0
11	38.105903549362544	39.0	38.0	39.0	37.0	39.0
12	38.07059169201445	39.0	38.0	39.0	37.0	39.0
13	38.06641173462247	39.0	38.0	39.0	37.0	39.0
14	39.57994381121779	41.0	40.0	41.0	37.0	41.0
15	39.56728498265368	41.0	40.0	41.0	37.0	41.0
16	39.5294855141462	41.0	39.0	41.0	37.0	41.0
17	39.5101723198249	41.0	39.0	41.0	37.0	41.0
18	39.474478526377084	41.0	39.0	41.0	37.0	41.0
19	39.4654273678857	41.0	39.0	41.0	37.0	41.0
20	39.42980746283873	41.0	39.0	41.0	37.0	41.0
21	39.404583511426694	41.0	39.0	41.0	36.0	41.0
22	39.363421818827426	41.0	39.0	41.0	36.0	41.0
23	39.3267740906897	41.0	39.0	41.0	36.0	41.0
24	39.29668909619288	41.0	39.0	41.0	36.0	41.0
25	39.25345527360547	41.0	39.0	41.0	36.0	41.0
26	39.16708936606534	40.0	39.0	41.0	36.0	41.0
27	39.107207056245166	40.0	39.0	41.0	36.0	41.0
28	39.05687146904	40.0	39.0	41.0	36.0	41.0
29	38.98206325603058	40.0	39.0	41.0	35.0	41.0
30	38.93286812251227	40.0	39.0	41.0	35.0	41.0
31	38.84690617322759	40.0	38.0	41.0	35.0	41.0
32	38.77726862588783	40.0	38.0	41.0	35.0	41.0
33	38.67539077383493	40.0	38.0	41.0	35.0	41.0
34	38.624933342950634	40.0	38.0	41.0	35.0	41.0
35	38.56346669782841	40.0	38.0	41.0	35.0	41.0
36	38.49945222840592	40.0	38.0	41.0	34.0	41.0
37	38.69006661137767	40.0	38.0	41.0	35.0	41.0
38	38.71338936042725	40.0	38.0	41.0	35.0	41.0
39	38.71489195914662	40.0	38.0	41.0	35.0	41.0
40	38.682934556452786	40.0	38.0	41.0	35.0	41.0
41	38.64459390462427	40.0	38.0	41.0	35.0	41.0
42	38.573104422605326	40.0	38.0	41.0	35.0	41.0
43	38.52810496909076	40.0	38.0	41.0	35.0	41.0
44	38.45570475675414	40.0	38.0	41.0	34.0	41.0
45	38.40507290724688	40.0	38.0	41.0	34.0	41.0
46	38.34583078858006	40.0	38.0	41.0	34.0	41.0
47	38.266255001969135	40.0	38.0	41.0	34.0	41.0
48	38.21729715127273	40.0	38.0	41.0	34.0	41.0
49	38.17178478364059	40.0	38.0	41.0	34.0	41.0
50	38.26747795343356	40.0	38.0	41.0	34.0	41.0
51	38.00938721291272	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	11.0
11	25.0
12	59.0
13	140.0
14	229.0
15	458.0
16	760.0
17	1143.0
18	2137.0
19	3867.0
20	6821.0
21	12007.0
22	19692.0
23	30710.0
24	45024.0
25	64239.0
26	88171.0
27	118632.0
28	157088.0
29	207251.0
30	268724.0
31	351054.0
32	456211.0
33	610078.0
34	864732.0
35	1283251.0
36	1931841.0
37	3212497.0
38	7181017.0
39	2.1165523E7
40	14601.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.093721359068674	27.363238331476808	28.496522008355896	24.04651830109862
2	21.92685289761553	27.174365840948354	31.546556866454438	19.352224394981675
3	20.927105982162423	26.152811292226495	32.718119871711885	20.20196285389919
4	21.78086742410283	25.963195019496567	32.09006583968353	20.165871716717067
5	25.79085787084444	26.354761547037803	28.177384973214863	19.676995608902896
6	21.99622258346607	26.03900740605884	31.454958943247302	20.509811067227787
7	25.682117243122182	26.655163174461983	27.59302352806169	20.069696054354143
8	21.733363613141226	26.6107907618028	31.19785880601174	20.457986819044237
9	21.148427334603113	26.727319200726463	31.655389433081993	20.46886403158843
10	21.58234254631136	26.57880482742452	31.403998257546146	20.43485436871798
11	25.44220436161524	26.462578241648195	27.59990315501109	20.49531424172547
12	21.4950069962214	30.587643225151496	27.290720481137605	20.626629297489497
13	23.676914659763206	26.97113517466903	28.274865166136298	21.077084999431467
14	23.069219220007454	27.166986020190144	28.52734541102111	21.236449348781296
15	23.221024538928503	27.211478525012183	28.228899493821146	21.338597442238168
16	23.140213516256726	27.171654650853093	28.193944830001392	21.494187002888786
17	23.07615986290512	27.30088361082738	28.10327231402262	21.519684212244876
18	22.830240427913637	27.242961998232918	28.340028517671414	21.58676905618204
19	22.57231441024651	27.555323883120963	28.532100039862524	21.34026166677
20	22.311981974064988	27.569702091417092	28.63292861913262	21.485387315385303
21	22.46722694986967	27.56829329207482	28.40682823334929	21.55765152470622
22	22.38270485408209	27.64070582610172	28.460491727701374	21.516097592114818
23	22.45660112936124	27.69599744826473	28.288178202339044	21.559223220034983
24	22.294986346653957	27.72607119636006	28.29339250916328	21.6855499478227
25	22.37953933324997	27.8201325865014	28.20360944969389	21.59671863055474
26	22.3640436788912	27.740662791511404	28.166376531386927	21.72891699821047
27	22.383171048278708	27.75896164374191	28.060491106741907	21.797376201237476
28	22.359813918777387	27.83549692533624	28.07546498454105	21.729224171345326
29	22.45296314273328	27.80679855181884	27.991125832445363	21.749112473002516
30	22.40544095810184	27.71949474717778	28.05027067565444	21.824793619065947
31	22.540363679316478	27.71159744597636	27.87738586624843	21.870653008458728
32	22.52521970118539	27.79795890511639	27.85310206524364	21.823719328454587
33	22.468649389356106	27.73720928315836	27.83669486746043	21.9574464600251
34	22.45883221784159	27.804685579787453	27.84085546126888	21.895626741102078
35	22.526779761547427	27.737405290126873	27.79263125182548	21.943183696500228
36	22.54720485851949	27.68961904038686	27.824438504441034	21.93873759665262
37	22.54510135029167	27.67602753714855	27.834177915816237	21.94469319674354
38	22.55994633208247	27.721031716263067	27.845043494940967	21.873978456713495
39	22.484321264727733	27.670016480419058	27.810391528003652	22.035270726849554
40	22.495070344382416	27.699462197434215	27.862077049931973	21.943390408251396
41	22.530399272463246	27.64945199428346	27.862771012205435	21.957377721047862
42	22.4443010014806	27.67204108814974	27.808717582805937	22.07494032756372
43	22.504997314161006	27.709010179415095	27.767630108327307	22.018362398096592
44	22.550947299170275	27.608265799597437	27.776348131277036	22.06443876995525
45	22.582875491358664	27.586944740888047	27.71129531590873	22.118884451844554
46	22.476071969769748	27.69490552731435	27.757462623493375	22.071559879422527
47	22.489337759392907	27.7375901871584	27.713318569302174	22.059753484146515
48	22.45997663499151	27.594501296078672	27.73870573139858	22.206816337531244
49	22.59049793590193	27.6213268540424	27.696304551032032	22.09187065902364
50	22.45832562951605	27.617200652758743	27.70931573408743	22.215157983637773
51	22.502506039035673	27.609224323500705	27.67752717533244	22.210742462131183
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	44.14023966016729
2	41.27907729259721
3	41.12906883606161
4	41.9467391408199
5	45.46785347974733
6	42.50603365069386
7	45.75181329747633
8	42.19135043218546
9	41.617291366191544
10	42.01719691502934
11	45.93751860334071
12	42.12163629371089
13	44.75399965919467
14	44.30566856878875
15	44.55962198116667
16	44.634400519145515
17	44.59584407514999
18	44.41700948409568
19	43.91257607701651
20	43.79736928945029
21	44.02487847457589
22	43.8988024461969
23	44.015824349396226
24	43.980536294476664
25	43.97625796380471
26	44.092960677101665
27	44.18054724951618
28	44.08903809012271
29	44.2020756157358
30	44.230234577167785
31	44.41101668777521
32	44.34893902963997
33	44.426095849381205
34	44.35445895894367
35	44.46996345804766
36	44.48594245517211
37	44.48979454703521
38	44.43392478879596
39	44.519591991577286
40	44.438460752633816
41	44.487776993511105
42	44.51924132904432
43	44.5233597122576
44	44.61538606912552
45	44.701759943203214
46	44.54763184919228
47	44.54909124353942
48	44.66679297252276
49	44.68236859492557
50	44.67348361315383
51	44.71324850116686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26048.0
1	15731.5
2	5415.0
3	7597.0
4	9779.0
5	17044.5
6	24310.0
7	39086.0
8	53862.0
9	41867.5
10	29873.0
11	32588.0
12	35303.0
13	46082.0
14	56861.0
15	77151.0
16	97441.0
17	135078.0
18	172715.0
19	220409.0
20	268103.0
21	325680.0
22	383257.0
23	459424.0
24	535591.0
25	632619.0
26	844244.0
27	958841.0
28	1058417.5
29	1157994.0
30	1262915.5
31	1367837.0
32	1483366.5
33	1598896.0
34	1710856.5
35	1822817.0
36	1970249.0
37	2117681.0
38	2225911.0
39	2334141.0
40	2438226.0
41	2542311.0
42	2590116.5
43	2637922.0
44	2656967.5
45	2676013.0
46	2651330.5
47	2626648.0
48	2571869.0
49	2517090.0
50	2410156.0
51	2303222.0
52	2140698.5
53	1978175.0
54	1819461.0
55	1660747.0
56	1503248.0
57	1345749.0
58	1191561.0
59	1037373.0
60	915494.0
61	793615.0
62	682144.5
63	570674.0
64	491376.5
65	412079.0
66	343649.5
67	275220.0
68	255532.5
69	235845.0
70	208546.0
71	181247.0
72	154221.5
73	127196.0
74	107210.5
75	72822.5
76	58420.0
77	55482.0
78	52544.0
79	52453.5
80	52363.0
81	45353.0
82	38343.0
83	34935.5
84	31528.0
85	28143.5
86	24759.0
87	29027.0
88	33295.0
89	21272.5
90	9250.0
91	5499.0
92	1748.0
93	1258.5
94	769.0
95	463.0
96	157.0
97	99.0
98	41.0
99	28.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27547118226376005
2	0.022822722748986588
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	7.559452733419364E-4
13	0.0
14	5.144627554688179E-4
15	0.0
16	1.8373669838172066E-5
17	5.249619953763447E-6
18	5.643341450295706E-4
19	0.005412358172330114
20	0.0
21	2.6248099768817236E-6
22	0.0
23	0.0
24	2.3623289791935512E-5
25	0.0
26	0.005181374894364522
27	0.004076329894097317
28	2.3623289791935512E-5
29	0.0
30	0.0
31	0.0032705132311946277
32	0.002089348741597852
33	3.674733967634413E-5
34	0.0
35	0.004680036188780113
36	0.0029476616040381757
37	2.073599881736562E-4
38	5.249619953763447E-6
39	1.4961416868225825E-4
40	0.0
41	3.3335086706397894E-4
42	1.0499239907526895E-5
43	8.136910928333343E-5
44	0.0
45	1.6273821856666685E-4
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	6.824505939892482E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3.8097996E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.99309564340805
#Duplication Level	Relative count
1	100.0
2	12.504658245415644
3	3.5274161837083486
4	1.6493401354425012
5	0.9586347807090814
6	0.6707873398527352
7	0.4992354052352253
8	0.3559542014161066
9	0.30262532286459604
10++	8.034670196224573
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TGTGT	7349540	3.485191	38.21453	4
TTTTT	11679195	3.4674737	4.2964754	16
TGTTT	6691375	2.5107226	30.24558	6
GTTTG	4965455	2.3546453	37.37214	7
CTTTG	4733330	2.3539288	39.196316	1
GTGTT	4639535	2.2000923	37.37961	5
TTTGA	5613610	2.1880107	30.959557	8
TTTGT	5550765	2.0827456	29.900513	2
TTGTG	4305280	2.0415869	37.391083	3
GAGGG	2586160	2.034754	10.518686	11
TGAGG	2974675	1.8518803	13.874398	10
TTGAG	3595375	1.7710649	22.899397	9
AGGGG	1971620	1.5512426	5.964935	12
TGAGC	2202725	1.4381155	5.2241993	10
GAGTG	2027925	1.2624822	5.436007	11
TGAGT	2519440	1.2410644	6.8125854	10
TTGAT	2669010	1.0402972	7.206736	9
TTGAC	1743975	0.90092915	6.2265196	9
>>END_MODULE