FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_9_ACTTGA_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_9_ACTTGA_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 26710200
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171582 0.6423838084327336 No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 133447 0.4996106356373221 No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 131632 0.4928154787309717 No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 79454 0.29746688530973187 No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63886 0.23918203532732815 No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51010 0.19097573211731847 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30022 0.11239900861843041 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 32363175 10.76092 14.422021 18
AAAAA 9493745 6.778718 8.17986 47
TGTGT 8929615 4.8136263 48.571033 4
GTTTG 6995795 3.7711751 48.067993 7
GTGTT 6760850 3.6445248 48.232937 5
TTTGA 7267015 3.5847375 44.15633 8
TGTTT 7926095 3.3556619 38.221493 6
TTGTG 6130455 3.3047023 48.184326 3
CTTTG 5135000 3.127746 54.590427 1
GGGGG 2692040 2.995596 8.305384 13
GAGGG 2837390 2.8892322 17.592487 11
TTGAG 4412615 2.7715201 32.412083 9
TTTGT 5713800 2.419045 38.079147 2
GAGAG 2584325 2.408087 5.524552 11
TGAGG 2990340 2.3914607 20.028173 10
AGGGG 1976510 2.0126228 10.079486 12
AACTT 3091140 2.0074916 30.76396 2
ACTTT 3384930 1.8866982 25.787325 3
TGAGC 1744460 1.5763589 7.358854 10
TAACT 2413195 1.567211 30.55391 1
ATTTT 3854115 1.4931564 5.6192555 12
TGAGA 1987020 1.4541411 5.685767 10
TTGAT 2776040 1.3693895 11.696542 9
GAGTG 1656560 1.3247987 7.346048 11
TGAGT 2055855 1.2912621 9.006869 10
TGATT 2331820 1.1502606 7.8872247 10
GATTT 2268130 1.1188433 6.7307315 11
TTGAC 1554740 1.1033955 8.374096 9
GTAAC 1094745 0.90525043 9.031113 1
GGTAA 1093520 0.80026 5.3407173 1
Produced by FastQC (version 0.10.1)