##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	140701_JD_B_9_ACTTGA_L004_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	26710200	
Filtered Sequences	0	
Sequence length	51	
%GC	44	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44235823767699	34.0	31.0	34.0	31.0	34.0
2	32.606961535293635	34.0	31.0	34.0	31.0	34.0
3	32.69184539988469	34.0	31.0	34.0	31.0	34.0
4	36.00034574806628	37.0	37.0	37.0	35.0	37.0
5	36.04913579830926	37.0	37.0	37.0	35.0	37.0
6	36.03263367552471	37.0	37.0	37.0	35.0	37.0
7	36.08081332225142	37.0	37.0	37.0	35.0	37.0
8	36.06126019273536	37.0	37.0	37.0	35.0	37.0
9	37.8419842981333	39.0	39.0	39.0	37.0	39.0
10	37.81743790761582	39.0	39.0	39.0	37.0	39.0
11	37.8188294733847	39.0	39.0	39.0	37.0	39.0
12	37.77776437465837	39.0	39.0	39.0	37.0	39.0
13	37.78967735172331	39.0	39.0	39.0	37.0	39.0
14	39.32995743199227	41.0	40.0	41.0	37.0	41.0
15	39.26469667018592	41.0	40.0	41.0	37.0	41.0
16	39.24031351318972	41.0	40.0	41.0	37.0	41.0
17	39.19242199609138	41.0	40.0	41.0	37.0	41.0
18	39.14522410914183	41.0	40.0	41.0	36.0	41.0
19	39.139728942501364	41.0	40.0	41.0	36.0	41.0
20	39.11744453429776	41.0	40.0	41.0	36.0	41.0
21	39.07566644952116	41.0	40.0	41.0	36.0	41.0
22	39.03927361083032	41.0	40.0	41.0	36.0	41.0
23	39.00029202327201	41.0	39.0	41.0	36.0	41.0
24	38.95737384969038	41.0	39.0	41.0	36.0	41.0
25	38.901527993051346	41.0	39.0	41.0	36.0	41.0
26	38.84185097827796	41.0	39.0	41.0	35.0	41.0
27	38.762284370764725	41.0	39.0	41.0	35.0	41.0
28	38.69335830506698	41.0	39.0	41.0	35.0	41.0
29	38.62672761716498	41.0	39.0	41.0	35.0	41.0
30	38.55610601942329	41.0	39.0	41.0	35.0	41.0
31	38.51228354710934	41.0	39.0	41.0	35.0	41.0
32	38.45176539299593	41.0	38.0	41.0	35.0	41.0
33	38.40082754902622	40.0	38.0	41.0	35.0	41.0
34	38.334660354471325	40.0	38.0	41.0	35.0	41.0
35	38.27157179654215	40.0	38.0	41.0	35.0	41.0
36	38.20040950648067	40.0	38.0	41.0	34.0	41.0
37	38.15792090661994	40.0	38.0	41.0	34.0	41.0
38	38.10790828971704	40.0	38.0	41.0	34.0	41.0
39	38.046917319975144	40.0	38.0	41.0	34.0	41.0
40	37.967098973425884	40.0	38.0	41.0	34.0	41.0
41	37.88740477420611	40.0	38.0	41.0	33.0	41.0
42	37.82226220694716	40.0	38.0	41.0	33.0	41.0
43	37.76656007817238	40.0	38.0	41.0	33.0	41.0
44	37.68597187591257	40.0	37.0	41.0	33.0	41.0
45	37.620741589355376	40.0	37.0	41.0	33.0	41.0
46	37.53950913883086	40.0	37.0	41.0	33.0	41.0
47	37.44746389019925	40.0	37.0	41.0	33.0	41.0
48	37.62949764509438	40.0	37.0	41.0	33.0	41.0
49	37.66029842532066	40.0	37.0	41.0	33.0	41.0
50	37.63015054174061	40.0	37.0	41.0	33.0	41.0
51	36.17140927435961	38.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	138784.0
3	2996.0
4	1889.0
5	1461.0
6	1840.0
7	3445.0
8	10092.0
9	15800.0
10	13732.0
11	13957.0
12	17220.0
13	22193.0
14	26533.0
15	29794.0
16	31898.0
17	33540.0
18	35468.0
19	37326.0
20	39053.0
21	41612.0
22	45286.0
23	50003.0
24	56744.0
25	65285.0
26	76234.0
27	90878.0
28	108989.0
29	132953.0
30	162893.0
31	201124.0
32	256978.0
33	344981.0
34	508846.0
35	865409.0
36	1485398.0
37	2133786.0
38	4291191.0
39	1.5301028E7
40	13561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.718864552810142	23.503399367501235	33.37670102404634	24.401035055642282
2	21.213086857869293	27.18640853700846	34.3935105977128	17.206994007409442
3	23.60959753251995	26.427187603213515	31.739978327938655	18.22323653632788
4	20.748030695697874	22.702035336795838	34.81529725163274	21.73463671587355
5	30.32910765413642	22.245985943509815	29.09122114864552	18.333685253708243
6	19.66222414498762	21.685049220752976	39.9282747604315	18.724451873827906
7	26.49740495243269	22.151983430783993	33.55970916916807	17.79090244761524
8	23.81199032693534	22.396840481539197	35.77246630700469	18.01870288452077
9	24.260322958480074	23.38659365602834	34.32644227119333	18.026641114298254
10	23.72951808819184	26.636446905655365	31.491596424256464	18.142438581896332
11	29.271300398628476	22.57947251957246	29.37178763296243	18.77743944883663
12	21.807242299651428	29.564186570777153	29.674468098481977	18.954103031089446
13	25.73849220356969	23.17747473905689	31.277990184930104	19.806042872443314
14	27.28695241663702	23.874417098572344	28.655454506177847	20.183175978612784
15	25.09465982809937	27.077826769643103	27.50325324020088	20.324260162056646
16	26.167805113808956	24.75730881559716	28.419860209456203	20.65502586113768
17	25.289701895917087	24.963986324591716	29.00240086149711	20.743910917994082
18	25.06627589026356	24.847967931461607	29.28219001611761	20.803566162157225
19	24.421833782647766	25.242206267512817	29.70972442926526	20.62623552057416
20	23.719717687465895	25.361788510454808	29.945722970529488	20.972770831549813
21	23.89274637672836	25.622882239176132	29.685736768461034	20.798634615634473
22	23.228707540024637	25.964457360093128	30.122428749571124	20.68440635031111
23	23.14508378233694	26.175279219861398	29.86369156455068	20.815945433250985
24	22.852654820977733	26.365123998269024	29.801149620112728	20.981071560640515
25	22.870861997982566	26.626658967926947	29.59210795611598	20.91037107797451
26	22.75317548412063	26.6045777484049	29.605859655309473	21.036387112164988
27	22.801272463545455	26.60088957974662	29.468240376316636	21.129597580391287
28	22.728359717558305	26.704738227617987	29.417187295996804	21.149714758826903
29	22.92479278630374	26.587112630372655	29.17130343649628	21.31679114682732
30	22.896530622408754	26.580305497387307	29.15668945067115	21.366474429532794
31	22.967590764259537	26.53603234840639	29.0054364110158	21.49094047631827
32	23.035036387447576	26.622654859521393	28.872429934006778	21.469878819024252
33	23.006784678847474	26.580188761875696	28.79832219367955	21.614704365597284
34	23.114654746000294	26.6216284571058	28.706598835033777	21.557117961860133
35	23.153678420396467	26.516913580632878	28.620797679279036	21.70861031969162
36	23.039286623478148	26.5827161853869	28.703139163073953	21.674858028061
37	23.06730581818079	26.45719671610958	28.688035117552847	21.78746234815679
38	22.953944257347864	26.569993445027766	28.782580965508764	21.693481332115606
39	22.976772632250476	26.43566839738054	28.707496801750725	21.880062168618256
40	23.020518515684152	26.55938775872983	28.668906413309102	21.75118731227691
41	23.055160794566614	26.499205816050935	28.625962335055988	21.819671054326463
42	22.943403897968672	26.438162223715118	28.66114445273748	21.957289425578725
43	22.99323334151346	26.6002840236526	28.52637378357681	21.880108851257138
44	23.073722797154833	26.50517360263356	28.514888750473723	21.906214849737886
45	23.03461012775475	26.51597781517921	28.45550798796628	21.993904069099766
46	22.847971788265575	26.723206474322513	28.338002451098536	22.090819286313373
47	23.03857966424598	26.507251655632878	28.431759548405804	22.022409131715335
48	22.882455910950668	26.405850369089368	28.3332348221036	22.378458897856362
49	22.945790045546463	26.533494628425192	28.391887557507523	22.128827768520825
50	22.897533797351137	26.548417162268752	28.405563016259105	22.148486024121006
51	22.975466426459334	26.49016287265038	28.328495977940367	22.205874722949922
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	43.11989960845243
2	38.42008086527874
3	41.83283406884783
4	42.48266741157142
5	48.66279290784467
6	38.38667601881553
7	44.288307400047934
8	41.83069321145611
9	42.28696407277833
10	41.871956670088174
11	48.04873984746511
12	40.76134533074087
13	45.544535076013005
14	47.47012839524981
15	45.41891999015602
16	46.82283097494663
17	46.033612813911176
18	45.869842052420786
19	45.04806930322193
20	44.69248851901571
21	44.69138099236283
22	43.91311389033575
23	43.96102921558792
24	43.83372638161825
25	43.78123307595707
26	43.78956259628562
27	43.93087004393674
28	43.87807447638521
29	44.24158393313106
30	44.26300505194155
31	44.458531240577805
32	44.50491520647183
33	44.62148904444476
34	44.67177270786043
35	44.86228874008809
36	44.714144651539144
37	44.854768166337585
38	44.64742558946347
39	44.85683480086873
40	44.771705827961064
41	44.87483184889307
42	44.900693323547394
43	44.87334219277059
44	44.97993764689272
45	45.028514196854516
46	44.93879107457895
47	45.060988795961315
48	45.26091480880704
49	45.07461781406729
50	45.04601982147214
51	45.18134114940926
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	238887.0
1	129089.0
2	19291.0
3	22887.0
4	26483.0
5	143793.5
6	261104.0
7	303046.5
8	344989.0
9	204006.5
10	63024.0
11	58758.0
12	54492.0
13	52290.5
14	50089.0
15	61690.0
16	73291.0
17	99906.0
18	126521.0
19	151254.5
20	175988.0
21	204860.0
22	233732.0
23	279410.0
24	325088.0
25	377619.5
26	498963.5
27	567776.0
28	625864.5
29	683953.0
30	739847.0
31	795741.0
32	867514.0
33	939287.0
34	1004224.5
35	1069162.0
36	1173035.5
37	1276909.0
38	1350447.5
39	1423986.0
40	1498255.0
41	1572524.0
42	1628090.0
43	1683656.0
44	1725347.5
45	1767039.0
46	1774760.0
47	1782481.0
48	1766040.5
49	1749600.0
50	1699734.0
51	1649868.0
52	1551910.5
53	1453953.0
54	1355207.0
55	1256461.0
56	1158708.0
57	1060955.0
58	956839.5
59	852724.0
60	768556.0
61	684388.0
62	594489.0
63	504590.0
64	439975.5
65	375361.0
66	315217.0
67	255073.0
68	236344.5
69	217616.0
70	193445.0
71	169274.0
72	143115.5
73	116957.0
74	98739.0
75	68328.0
76	56135.0
77	51979.5
78	47824.0
79	49315.0
80	50806.0
81	44449.0
82	38092.0
83	35742.5
84	33393.0
85	29283.5
86	25174.0
87	27470.0
88	29766.0
89	19894.5
90	10023.0
91	6510.5
92	2998.0
93	2349.0
94	1700.0
95	1251.5
96	803.0
97	595.5
98	388.0
99	250.5
100	113.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.8367889420521
2	0.5587116532261084
3	0.5433205292360221
4	0.5522983729062305
5	0.5304715052676505
6	0.5488839469565934
7	0.5420813022740376
8	0.5424369716437916
9	0.5316957566772245
10	0.5825901715449529
11	0.5526802494927031
12	0.5445897073028281
13	0.5364916773367477
14	0.5196965953081594
15	0.5836908746471385
16	0.5365366039939798
17	0.5543500235864951
18	0.5376260754318575
19	0.5522609340252038
20	0.5525304939685962
21	0.585937207508742
22	0.5931441921063864
23	0.5654094690417893
24	0.5594342236299242
25	0.5421636678122964
26	0.5636386099692252
27	0.5759597457151201
28	0.5545297302154233
29	0.6182656812753181
30	0.6198156509498244
31	0.6009501988004582
32	0.6292727122971749
33	0.6197594926282843
34	0.5973710417743034
35	0.603548457143713
36	0.6282506308451453
37	0.5966821663634116
38	0.6080598423074332
39	0.5863303157595227
40	0.5907555914968814
41	0.5978090766823162
42	0.5908080059303188
43	0.5764127561755434
44	0.5886627580474875
45	0.5671990475548666
46	0.5824591354613593
47	0.5744509588097431
48	0.6177527686052519
49	0.6087000471729901
50	0.593365081504444
51	0.565035080231522
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.67102E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	41.034926766457424
#Duplication Level	Relative count
1	100.0
2	17.43172133509243
3	6.671670988581047
4	3.702439986048557
5	2.4634911969428144
6	1.665074382421182
7	1.3147718484145399
8	1.031951837192727
9	0.8621081843410218
10++	16.502888858559665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171582	0.6423838084327336	No Hit
TTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	133447	0.4996106356373221	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	131632	0.4928154787309717	No Hit
TAACTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79454	0.29746688530973187	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63886	0.23918203532732815	No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	51010	0.19097573211731847	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30022	0.11239900861843041	No Hit
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	32363175	10.76092	14.422021	18
AAAAA	9493745	6.778718	8.17986	47
TGTGT	8929615	4.8136263	48.571033	4
GTTTG	6995795	3.7711751	48.067993	7
GTGTT	6760850	3.6445248	48.232937	5
TTTGA	7267015	3.5847375	44.15633	8
TGTTT	7926095	3.3556619	38.221493	6
TTGTG	6130455	3.3047023	48.184326	3
CTTTG	5135000	3.127746	54.590427	1
GGGGG	2692040	2.995596	8.305384	13
GAGGG	2837390	2.8892322	17.592487	11
TTGAG	4412615	2.7715201	32.412083	9
TTTGT	5713800	2.419045	38.079147	2
GAGAG	2584325	2.408087	5.524552	11
TGAGG	2990340	2.3914607	20.028173	10
AGGGG	1976510	2.0126228	10.079486	12
AACTT	3091140	2.0074916	30.76396	2
ACTTT	3384930	1.8866982	25.787325	3
TGAGC	1744460	1.5763589	7.358854	10
TAACT	2413195	1.567211	30.55391	1
ATTTT	3854115	1.4931564	5.6192555	12
TGAGA	1987020	1.4541411	5.685767	10
TTGAT	2776040	1.3693895	11.696542	9
GAGTG	1656560	1.3247987	7.346048	11
TGAGT	2055855	1.2912621	9.006869	10
TGATT	2331820	1.1502606	7.8872247	10
GATTT	2268130	1.1188433	6.7307315	11
TTGAC	1554740	1.1033955	8.374096	9
GTAAC	1094745	0.90525043	9.031113	1
GGTAA	1093520	0.80026	5.3407173	1
>>END_MODULE