FastQCFastQC Report
Tue 12 Aug 2014
140701_JD_B_9_ACTTGA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 140701_JD_B_9_ACTTGA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 26710200
Filtered Sequences 0
Sequence length 51
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 158140 0.5920584645566113 No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82619 0.30931629115468995 No Hit
TGTGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 48219 0.18052654042275984 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37150 0.1390854430142792 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 24739215 9.988469 13.00193 18
AAAAA 11771840 7.5736833 9.106115 47
TGTGT 6224195 3.90355 56.038948 4
GTTTG 4860365 3.0482137 55.622253 7
TGTTT 5794705 2.9159236 45.117126 6
GTGTT 4597365 2.8832715 55.581337 5
TTTGA 5195790 2.8698971 49.191525 8
CTTTG 4184010 2.8120332 59.452 1
TTGTG 3945895 2.474697 55.476864 3
GAGGG 2289830 2.4485743 16.770914 11
TTTGT 4662955 2.3464215 44.7441 2
GGGGG 1899405 2.3061602 6.99837 13
TTGAG 3165520 2.1791725 34.14373 9
GAGAG 2242500 2.1119356 5.1700087 11
TGAGG 2394815 2.0547085 20.637424 10
AGGGG 1615275 1.7272552 9.596168 12
ATTTT 3545270 1.571203 6.948163 12
TGAGC 1601050 1.47209 7.4525084 10
ACTTT 2367950 1.4016463 5.5554156 5
TGAGA 1842880 1.3925587 5.754972 10
AACTT 2130935 1.3845417 6.0861497 4
GAGTG 1456310 1.249488 7.416614 11
TTGAT 2257895 1.2471493 13.693929 9
TGAGT 1756440 1.2091491 9.53137 10
TGATT 2006235 1.1081449 9.435777 10
GATTT 1975120 1.0909585 8.1214905 11
TTGAC 1329475 0.98079425 9.066769 9
TAACT 1448835 0.94135785 5.7832828 3
GGTAA 1091600 0.8248595 6.4768896 1
GTAAC 981425 0.7947394 6.956533 2
Produced by FastQC (version 0.10.1)